A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis
Abstract The type VI secretion system (T6SS) is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria, host organisms, and the environment. Yersinia pseudotuberculosis T6SS4 was recently reported to be involved in manganese acq...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-01-01
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| Series: | Stress Biology |
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| Online Access: | https://doi.org/10.1007/s44154-022-00081-y |
| _version_ | 1797958833540169728 |
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| author | Yuxin Zuo Changfu Li Danyang Yu Kenan Wang Yuqi Liu Zhiyan Wei Yantao Yang Yao Wang Xihui Shen Lingfang Zhu |
| author_facet | Yuxin Zuo Changfu Li Danyang Yu Kenan Wang Yuqi Liu Zhiyan Wei Yantao Yang Yao Wang Xihui Shen Lingfang Zhu |
| author_sort | Yuxin Zuo |
| collection | DOAJ |
| description | Abstract The type VI secretion system (T6SS) is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria, host organisms, and the environment. Yersinia pseudotuberculosis T6SS4 was recently reported to be involved in manganese acquisition; however, the underlying regulatory mechanism still remains unclear. In this study, we discovered that T6SS4 is regulated by ferric uptake regulator (Fur) in response to manganese ions (Mn2+), and this negative regulation of Fur was proceeded by specifically recognizing the promoter region of T6SS4 in Y. pseudotuberculosis. Furthermore, T6SS4 is induced by low Mn2+ and oxidative stress conditions via Fur, acting as a Mn2+-responsive transcriptional regulator to maintain intracellular manganese homeostasis, which plays important role in the transport of Mn2+ for survival under oxidative stress. Our results provide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y. pseudotuberculosis. This study provides new insights into the regulation of T6SS4 via the Mn2+-dependent transcriptional regulator Fur, and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y. pseudotuberculosis. |
| first_indexed | 2024-04-11T00:24:24Z |
| format | Article |
| id | doaj.art-c378ee6c78854f28b5028af24d619c89 |
| institution | Directory Open Access Journal |
| issn | 2731-0450 |
| language | English |
| last_indexed | 2024-04-11T00:24:24Z |
| publishDate | 2023-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Stress Biology |
| spelling | doaj.art-c378ee6c78854f28b5028af24d619c892023-01-08T12:05:01ZengSpringerStress Biology2731-04502023-01-013111510.1007/s44154-022-00081-yA Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosisYuxin Zuo0Changfu Li1Danyang Yu2Kenan Wang3Yuqi Liu4Zhiyan Wei5Yantao Yang6Yao Wang7Xihui Shen8Lingfang Zhu9State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F UniversityAbstract The type VI secretion system (T6SS) is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria, host organisms, and the environment. Yersinia pseudotuberculosis T6SS4 was recently reported to be involved in manganese acquisition; however, the underlying regulatory mechanism still remains unclear. In this study, we discovered that T6SS4 is regulated by ferric uptake regulator (Fur) in response to manganese ions (Mn2+), and this negative regulation of Fur was proceeded by specifically recognizing the promoter region of T6SS4 in Y. pseudotuberculosis. Furthermore, T6SS4 is induced by low Mn2+ and oxidative stress conditions via Fur, acting as a Mn2+-responsive transcriptional regulator to maintain intracellular manganese homeostasis, which plays important role in the transport of Mn2+ for survival under oxidative stress. Our results provide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y. pseudotuberculosis. This study provides new insights into the regulation of T6SS4 via the Mn2+-dependent transcriptional regulator Fur, and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y. pseudotuberculosis.https://doi.org/10.1007/s44154-022-00081-yType VI secretion systemFurManganeseOxidative stressVirulence |
| spellingShingle | Yuxin Zuo Changfu Li Danyang Yu Kenan Wang Yuqi Liu Zhiyan Wei Yantao Yang Yao Wang Xihui Shen Lingfang Zhu A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis Stress Biology Type VI secretion system Fur Manganese Oxidative stress Virulence |
| title | A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis |
| title_full | A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis |
| title_fullStr | A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis |
| title_full_unstemmed | A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis |
| title_short | A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis |
| title_sort | fur regulated type vi secretion system contributes to oxidative stress resistance and virulence in yersinia pseudotuberculosis |
| topic | Type VI secretion system Fur Manganese Oxidative stress Virulence |
| url | https://doi.org/10.1007/s44154-022-00081-y |
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