Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita
Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and fun...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2022-12-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-08-2022-0105.pdf |
| _version_ | 1828166456657838080 |
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| author | Wenjie Tong Junying Li Wenfeng Cong Cuiping Zhang Zhaoli Xu Xiaolong Chen Min Yang Jiani Liu Lei Yu Xiaopeng Deng |
| author_facet | Wenjie Tong Junying Li Wenfeng Cong Cuiping Zhang Zhaoli Xu Xiaolong Chen Min Yang Jiani Liu Lei Yu Xiaopeng Deng |
| author_sort | Wenjie Tong |
| collection | DOAJ |
| description | Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations. |
| first_indexed | 2024-04-12T02:01:40Z |
| format | Article |
| id | doaj.art-c37b0ef8ba5e41069234be86f0ffd313 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-12T02:01:40Z |
| publishDate | 2022-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-c37b0ef8ba5e41069234be86f0ffd3132022-12-22T03:52:40ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802022-12-0138658359210.5423/PPJ.OA.08.2022.01052353Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognitaWenjie Tong0Junying Li1Wenfeng Cong2Cuiping Zhang3Zhaoli Xu4Xiaolong Chen5Min Yang6Jiani Liu7Lei Yu8Xiaopeng Deng9 Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, Yunnan, China Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, Yunnan, China National Academy of Agriculture Green Development, China Agricultural University, Beijing 100193, China Yuxi Branch of Yunnan Provincial Tobacco Company, Yuxi 653100, Yunnan, China Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, Yunnan, China Tobacco Leaf Purchase Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450000, China College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming 650214, Yunnan, China College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming 650214, Yunnan, China College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming 650214, Yunnan, China Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, Yunnan, ChinaRoot-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.http://www.ppjonline.org/upload/pdf/PPJ-OA-08-2022-0105.pdfpicrustroot-knot nematode diseasesoil bacterial communitytobacco16s rrna gene sequencing |
| spellingShingle | Wenjie Tong Junying Li Wenfeng Cong Cuiping Zhang Zhaoli Xu Xiaolong Chen Min Yang Jiani Liu Lei Yu Xiaopeng Deng Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita The Plant Pathology Journal picrust root-knot nematode disease soil bacterial community tobacco 16s rrna gene sequencing |
| title | Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita |
| title_full | Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita |
| title_fullStr | Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita |
| title_full_unstemmed | Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita |
| title_short | Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita |
| title_sort | bacterial community structure and function shift in rhizosphere soil of tobacco plants infected by meloidogyne incognita |
| topic | picrust root-knot nematode disease soil bacterial community tobacco 16s rrna gene sequencing |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-08-2022-0105.pdf |
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