Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields
<i>Ammannia</i><i>auriculata</i> is a troublesome broadleaf weed, widely distributed in the paddy fields of southern China. In this study, 10 biotypes of <i>A. auriculata</i> were sampled from Yangzhou City, China, where the paddy fields were seriously infested wi...
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MDPI AG
2022-07-01
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| Series: | Plants |
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| author | Longwei Liu Peng Wan Yang Li Zhiwen Duan Cheng Peng Shuzhong Yuan Wei Deng |
| author_facet | Longwei Liu Peng Wan Yang Li Zhiwen Duan Cheng Peng Shuzhong Yuan Wei Deng |
| author_sort | Longwei Liu |
| collection | DOAJ |
| description | <i>Ammannia</i><i>auriculata</i> is a troublesome broadleaf weed, widely distributed in the paddy fields of southern China. In this study, 10 biotypes of <i>A. auriculata</i> were sampled from Yangzhou City, China, where the paddy fields were seriously infested with <i>A. auriculata</i>, and their resistance levels to acetolactate synthase (ALS) inhibitor bensulfuron-methyl were determined. The whole-plant response assays showed that nine <i>A. auriculata</i> biotypes were highly resistant (from 16.4- to 183.1-fold) to bensulfuron-methyl in comparison with a susceptible YZ-S biotype, and only one YZ-6 biotype was susceptible. <i>ALS</i> gene sequencing revealed that three <i>ALS</i> gene copies existed in <i>A. auriculata</i>, and four different amino acid substitutions (Pro197-Leu, -Ala, -Ser, and -His) at site 197 in the <i>AaALS1</i> or <i>2</i> genes were found in eight resistant biotypes. In addition, no amino acid mutations in three <i>ALS</i> genes were found in the YZ-3 biotype. These results suggested that target-site mutations or non-target-site resistance mechanisms were involved in tested resistant <i>A. auriculata</i> biotypes. Finally, a cleaved amplified polymorphic sequence (CAPS) marker was identified to rapidly detect the Pro197 mutations in <i>A. auriculata</i>. |
| first_indexed | 2024-03-09T12:17:09Z |
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| id | doaj.art-c37b31adae21454093cf11ae91d0ff81 |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T12:17:09Z |
| publishDate | 2022-07-01 |
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| series | Plants |
| spelling | doaj.art-c37b31adae21454093cf11ae91d0ff812023-11-30T22:45:06ZengMDPI AGPlants2223-77472022-07-011115192610.3390/plants11151926Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy FieldsLongwei Liu0Peng Wan1Yang Li2Zhiwen Duan3Cheng Peng4Shuzhong Yuan5Wei Deng6College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225109, China<i>Ammannia</i><i>auriculata</i> is a troublesome broadleaf weed, widely distributed in the paddy fields of southern China. In this study, 10 biotypes of <i>A. auriculata</i> were sampled from Yangzhou City, China, where the paddy fields were seriously infested with <i>A. auriculata</i>, and their resistance levels to acetolactate synthase (ALS) inhibitor bensulfuron-methyl were determined. The whole-plant response assays showed that nine <i>A. auriculata</i> biotypes were highly resistant (from 16.4- to 183.1-fold) to bensulfuron-methyl in comparison with a susceptible YZ-S biotype, and only one YZ-6 biotype was susceptible. <i>ALS</i> gene sequencing revealed that three <i>ALS</i> gene copies existed in <i>A. auriculata</i>, and four different amino acid substitutions (Pro197-Leu, -Ala, -Ser, and -His) at site 197 in the <i>AaALS1</i> or <i>2</i> genes were found in eight resistant biotypes. In addition, no amino acid mutations in three <i>ALS</i> genes were found in the YZ-3 biotype. These results suggested that target-site mutations or non-target-site resistance mechanisms were involved in tested resistant <i>A. auriculata</i> biotypes. Finally, a cleaved amplified polymorphic sequence (CAPS) marker was identified to rapidly detect the Pro197 mutations in <i>A. auriculata</i>.https://www.mdpi.com/2223-7747/11/15/1926<i>Ammannia auriculata</i>target-site resistancebensulfuron-methylamino acid substitutionsCAPS |
| spellingShingle | Longwei Liu Peng Wan Yang Li Zhiwen Duan Cheng Peng Shuzhong Yuan Wei Deng Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields Plants <i>Ammannia auriculata</i> target-site resistance bensulfuron-methyl amino acid substitutions CAPS |
| title | Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields |
| title_full | Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields |
| title_fullStr | Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields |
| title_full_unstemmed | Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields |
| title_short | Occurrence of Bensulfuron-Methyl Resistance and Target-Site Resistance Mechanisms in <i>Ammannia auriculata</i> Biotypes from Paddy Fields |
| title_sort | occurrence of bensulfuron methyl resistance and target site resistance mechanisms in i ammannia auriculata i biotypes from paddy fields |
| topic | <i>Ammannia auriculata</i> target-site resistance bensulfuron-methyl amino acid substitutions CAPS |
| url | https://www.mdpi.com/2223-7747/11/15/1926 |
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