miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines
Objective To investigate the effect of miR-205-5p on the proliferation, migration and invasion of gastric cancer cells by targeting at RAS oncogene family (RAP2B). Methods RT-qPCR and Western blot were used to detect the expression of miR-205-5p and RAP2B in gastric cancer cell lines AGS, MGC803,...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-08-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190612.pdf |
| _version_ | 1827390478510718976 |
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| author | YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun |
| author_facet | YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun |
| author_sort | YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun |
| collection | DOAJ |
| description | Objective To investigate the effect of miR-205-5p on the proliferation, migration and invasion of gastric cancer cells by targeting at RAS oncogene family (RAP2B). Methods RT-qPCR and Western blot were used to detect the expression of miR-205-5p and RAP2B in gastric cancer cell lines AGS, MGC803, MKN-28, SGC-7901 and normal gastric mucosal cell line GES-1. Over expressing miR-205-5p, inhibition of RAP2B, cell viability was detected by MTT assay; cell migration and invasion ability were detected by Transwell method; The expression of RAP2B, cyclin D1, MMP-2, MMP-9 GSK-3β and β-catenin proteins were detected by Western blot. The dual luciferase reporter gene assay was used to verify the targeting of miR-205-5p to RAP2B. Results Compared with normal gastric mucosal cells, the expression of miR-205-5p was significantly decreased in four gastric cancer cell lines, and the expression of RAP2B was significantly increased (P＜0.05). The over-expression of miR-205-5p or inhibition of RAP2B inhibited the proliferation, migration and invasion of AGS cells and inhibited the expression of cyclin D1, MMP-2 and MMP-9 proteins (P＜0.05). miR-205-5pnegatively regulated the expression of RAP2B. Conclusions miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cells in vitro by targeting at RAP2B. |
| first_indexed | 2024-03-08T16:53:19Z |
| format | Article |
| id | doaj.art-c37b73fc0fd14abbb06b9aca659d3de4 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:19Z |
| publishDate | 2020-08-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-c37b73fc0fd14abbb06b9aca659d3de42024-01-05T03:17:00ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-08-0140810681075miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell linesYANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun0Department of General Surgery, Gansu Provincial People's Hospital, Lanzhou 730000, ChinaObjective To investigate the effect of miR-205-5p on the proliferation, migration and invasion of gastric cancer cells by targeting at RAS oncogene family (RAP2B). Methods RT-qPCR and Western blot were used to detect the expression of miR-205-5p and RAP2B in gastric cancer cell lines AGS, MGC803, MKN-28, SGC-7901 and normal gastric mucosal cell line GES-1. Over expressing miR-205-5p, inhibition of RAP2B, cell viability was detected by MTT assay; cell migration and invasion ability were detected by Transwell method; The expression of RAP2B, cyclin D1, MMP-2, MMP-9 GSK-3β and β-catenin proteins were detected by Western blot. The dual luciferase reporter gene assay was used to verify the targeting of miR-205-5p to RAP2B. Results Compared with normal gastric mucosal cells, the expression of miR-205-5p was significantly decreased in four gastric cancer cell lines, and the expression of RAP2B was significantly increased (P＜0.05). The over-expression of miR-205-5p or inhibition of RAP2B inhibited the proliferation, migration and invasion of AGS cells and inhibited the expression of cyclin D1, MMP-2 and MMP-9 proteins (P＜0.05). miR-205-5pnegatively regulated the expression of RAP2B. Conclusions miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cells in vitro by targeting at RAP2B.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190612.pdfmir-205-5p|rap2b|gastric cancer|proliferation|migration |
| spellingShingle | YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines Jichu yixue yu linchuang mir-205-5p|rap2b|gastric cancer|proliferation|migration |
| title | miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines |
| title_full | miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines |
| title_fullStr | miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines |
| title_full_unstemmed | miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines |
| title_short | miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines |
| title_sort | mir 205 5p inhibits the proliferation migration and invasion of gastric cancer cell lines |
| topic | mir-205-5p|rap2b|gastric cancer|proliferation|migration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a190612.pdf |
| work_keys_str_mv | AT yangyilihexueyuanpanxinminmajianxun mir2055pinhibitstheproliferationmigrationandinvasionofgastriccancercelllines |