Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family
The present study aimed to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of Lamiaceae medicinal plants growing wild in Croatia. Using Ellman’s colorimetric assay all tested ethanolic extracts and their hydroxycinnamic acid constituents demonstrated in vitro AChE inhibito...
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MDPI AG
2014-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/1/767 |
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| author | Sanda Vladimir-Knežević Biljana Blažeković Marija Kindl Jelena Vladić Agnieszka D. Lower-Nedza Adelheid H. Brantner |
| author_facet | Sanda Vladimir-Knežević Biljana Blažeković Marija Kindl Jelena Vladić Agnieszka D. Lower-Nedza Adelheid H. Brantner |
| author_sort | Sanda Vladimir-Knežević |
| collection | DOAJ |
| description | The present study aimed to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of Lamiaceae medicinal plants growing wild in Croatia. Using Ellman’s colorimetric assay all tested ethanolic extracts and their hydroxycinnamic acid constituents demonstrated in vitro AChE inhibitory properties in a dose dependent manner. The extracts of Mentha x piperita, M. longifolia, Salvia officinalis, Satureja montana, Teucrium arduini, T. chamaedrys, T. montanum, T. polium and Thymus vulgaris at 1 mg/mL showed strong inhibitory activity against AChE. The antioxidant potential of the investigated Lamiaceae species was assessed by DPPH• scavenging activity and total antioxidant capacity assays, in comparison with hydroxycinnamic acids and trolox. The extracts differed greatly in their total hydroxycinnamic derivatives content, determined spectrophotometrically. Rosmarinic acid was found to be the predominant constituent in most of the investigated medicinal plants (by RP-HPLC) and had a substantial influence on their AChE inhibitory and antioxidant properties, with the exception of Teucrium species. These findings indicate that Lamiaceae species are a rich source of various natural AChE inhibitors and antioxidants that could be useful in the prevention and treatment of Alzheimer’s and other related diseases. |
| first_indexed | 2024-12-11T00:35:41Z |
| format | Article |
| id | doaj.art-c37bac2d8e12450ca1fe766b3d42e297 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T00:35:41Z |
| publishDate | 2014-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c37bac2d8e12450ca1fe766b3d42e2972022-12-22T01:27:10ZengMDPI AGMolecules1420-30492014-01-0119176778210.3390/molecules19010767molecules19010767Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae FamilySanda Vladimir-Knežević0Biljana Blažeković1Marija Kindl2Jelena Vladić3Agnieszka D. Lower-Nedza4Adelheid H. Brantner5Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, Zagreb 10000, CroatiaDepartment of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, Zagreb 10000, CroatiaDepartment of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, Zagreb 10000, CroatiaDepartment of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, SerbiaDepartment of Pharmacognosy, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitaetsplatz 4, Graz 8010, AustriaDepartment of Pharmacognosy, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitaetsplatz 4, Graz 8010, AustriaThe present study aimed to evaluate acetylcholinesterase (AChE) inhibitory and antioxidant activities of Lamiaceae medicinal plants growing wild in Croatia. Using Ellman’s colorimetric assay all tested ethanolic extracts and their hydroxycinnamic acid constituents demonstrated in vitro AChE inhibitory properties in a dose dependent manner. The extracts of Mentha x piperita, M. longifolia, Salvia officinalis, Satureja montana, Teucrium arduini, T. chamaedrys, T. montanum, T. polium and Thymus vulgaris at 1 mg/mL showed strong inhibitory activity against AChE. The antioxidant potential of the investigated Lamiaceae species was assessed by DPPH• scavenging activity and total antioxidant capacity assays, in comparison with hydroxycinnamic acids and trolox. The extracts differed greatly in their total hydroxycinnamic derivatives content, determined spectrophotometrically. Rosmarinic acid was found to be the predominant constituent in most of the investigated medicinal plants (by RP-HPLC) and had a substantial influence on their AChE inhibitory and antioxidant properties, with the exception of Teucrium species. These findings indicate that Lamiaceae species are a rich source of various natural AChE inhibitors and antioxidants that could be useful in the prevention and treatment of Alzheimer’s and other related diseases.http://www.mdpi.com/1420-3049/19/1/767Croatian medicinal plantsLamiaceaeacetylcholinesterase inhibitionantioxidanthydroxycinnamic derivativesrosmarinic acid |
| spellingShingle | Sanda Vladimir-Knežević Biljana Blažeković Marija Kindl Jelena Vladić Agnieszka D. Lower-Nedza Adelheid H. Brantner Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family Molecules Croatian medicinal plants Lamiaceae acetylcholinesterase inhibition antioxidant hydroxycinnamic derivatives rosmarinic acid |
| title | Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family |
| title_full | Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family |
| title_fullStr | Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family |
| title_full_unstemmed | Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family |
| title_short | Acetylcholinesterase Inhibitory, Antioxidant and Phytochemical Properties of Selected Medicinal Plants of the Lamiaceae Family |
| title_sort | acetylcholinesterase inhibitory antioxidant and phytochemical properties of selected medicinal plants of the lamiaceae family |
| topic | Croatian medicinal plants Lamiaceae acetylcholinesterase inhibition antioxidant hydroxycinnamic derivatives rosmarinic acid |
| url | http://www.mdpi.com/1420-3049/19/1/767 |
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