The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages
Objective(s)Using herbal medicines as a complementary treatment method is increasing in wide variety of diseases. MS14-an herbal-marine preparation-is reported to have anti-inflammatory and immunomodulatory activities; however, the mechanism underlying its therapeutic effect is not known. Macrophage...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2011-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a12.pdf |
| _version_ | 1818542356820918272 |
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| author | Roya Yaraee Nayere Askari Mohsen Naseri |
| author_facet | Roya Yaraee Nayere Askari Mohsen Naseri |
| author_sort | Roya Yaraee |
| collection | DOAJ |
| description | Objective(s)Using herbal medicines as a complementary treatment method is increasing in wide variety of diseases. MS14-an herbal-marine preparation-is reported to have anti-inflammatory and immunomodulatory activities; however, the mechanism underlying its therapeutic effect is not known. Macrophages play an important role in host defense mechanisms and carry out their role by producing various mediators including proinflammatory cytokines (TNF, IL-1). In this study the effects of orally administered MS14 on TNF and IL-1 production of BALB/c mice peritoneal macrophages were evaluated. Materials and Methods MS14 at 100 mg/kg was orally administered for 5 days to BALB/c mice in MS14 group. Sterile normal saline was administered to mice in control group. Peritoneal macrophage were isolated from control and MS14 groups and cultured, then the supernatants were collected and the cytokines IL-1 and TNF were measured by ELISA test.ResultsSignificant decrease in TNF and IL-1 production of macrophages both at the presence and absence of stimulators was observed. TNF levels were 64.7±4.6 and 51.1±4.2 pg/ml in drug and control groups respectively (P< 0.05) and 298.7±31.3 and 177.0±26.5 pg/ml in stimulated (PMA+fMLP) cultures of drug and control groups respectively (P< 0.007). The IL-1 levels was 130.1±2.8 pg/ml in control and 65.1±5.6 in MS14 group (P< 0.000).Conclusion It could be concluded that MS14 is able to cause a decline in some inflammatory responses of immune system, which could be considered as at least one of its immunomodulatory mechanisms. |
| first_indexed | 2024-12-11T22:21:00Z |
| format | Article |
| id | doaj.art-c37d4faffed140cdb5672824023fda18 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-11T22:21:00Z |
| publishDate | 2011-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c37d4faffed140cdb5672824023fda182022-12-22T00:48:27ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742011-01-011448The Effect of MS14 on Production of Pro-inflammatory Cytokines by MacrophagesRoya YaraeeNayere AskariMohsen NaseriObjective(s)Using herbal medicines as a complementary treatment method is increasing in wide variety of diseases. MS14-an herbal-marine preparation-is reported to have anti-inflammatory and immunomodulatory activities; however, the mechanism underlying its therapeutic effect is not known. Macrophages play an important role in host defense mechanisms and carry out their role by producing various mediators including proinflammatory cytokines (TNF, IL-1). In this study the effects of orally administered MS14 on TNF and IL-1 production of BALB/c mice peritoneal macrophages were evaluated. Materials and Methods MS14 at 100 mg/kg was orally administered for 5 days to BALB/c mice in MS14 group. Sterile normal saline was administered to mice in control group. Peritoneal macrophage were isolated from control and MS14 groups and cultured, then the supernatants were collected and the cytokines IL-1 and TNF were measured by ELISA test.ResultsSignificant decrease in TNF and IL-1 production of macrophages both at the presence and absence of stimulators was observed. TNF levels were 64.7±4.6 and 51.1±4.2 pg/ml in drug and control groups respectively (P< 0.05) and 298.7±31.3 and 177.0±26.5 pg/ml in stimulated (PMA+fMLP) cultures of drug and control groups respectively (P< 0.007). The IL-1 levels was 130.1±2.8 pg/ml in control and 65.1±5.6 in MS14 group (P< 0.000).Conclusion It could be concluded that MS14 is able to cause a decline in some inflammatory responses of immune system, which could be considered as at least one of its immunomodulatory mechanisms.http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a12.pdfBALB/c miceInterleukin-1beta (IL-1)MacrophagesMS14Tumor necrosis factor-alpha (TNF) |
| spellingShingle | Roya Yaraee Nayere Askari Mohsen Naseri The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages Iranian Journal of Basic Medical Sciences BALB/c mice Interleukin-1beta (IL-1) Macrophages MS14 Tumor necrosis factor-alpha (TNF) |
| title | The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages |
| title_full | The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages |
| title_fullStr | The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages |
| title_full_unstemmed | The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages |
| title_short | The Effect of MS14 on Production of Pro-inflammatory Cytokines by Macrophages |
| title_sort | effect of ms14 on production of pro inflammatory cytokines by macrophages |
| topic | BALB/c mice Interleukin-1beta (IL-1) Macrophages MS14 Tumor necrosis factor-alpha (TNF) |
| url | http://www.mums.ac.ir/shares/basic_medical/basicmedjou/89/winter/a12.pdf |
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