Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5
Curcumin is an active ingredient isolated from <i>Curcuma longa</i>. It has several pharmacological effects, including anticancer, anti-inflammatory, and antioxidant effects. Due to its low bioavailability, chemical structure instability, and easy oxidation, the application of curcumin h...
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MDPI AG
2022-10-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/11/357 |
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| author | Simin Sharifi Elaheh Dalir Abdolahinia Mohammad Ali Ghavimi Solmaz Maleki Dizaj Michael Aschner Luciano Saso Haroon Khan |
| author_facet | Simin Sharifi Elaheh Dalir Abdolahinia Mohammad Ali Ghavimi Solmaz Maleki Dizaj Michael Aschner Luciano Saso Haroon Khan |
| author_sort | Simin Sharifi |
| collection | DOAJ |
| description | Curcumin is an active ingredient isolated from <i>Curcuma longa</i>. It has several pharmacological effects, including anticancer, anti-inflammatory, and antioxidant effects. Due to its low bioavailability, chemical structure instability, and easy oxidation, the application of curcumin has been limited. In this study, to overcome these limitations, curcumin-loaded mesoporous silica nanoparticles (Cur-MSN) were prepared, and the anticancerous effect of Cur-MSNs on head and neck cancer cells, HN5, was investigated. Transmission electron microscopy (TEM) revealed rod-shaped mesoporous nanoparticles with average particle size smaller than 100 nm. Higher cytotoxicity of Cur-MSNs was seen in treated cancer cells compared with free curcumin. The expression of <i>Bcl-2</i> was significantly reduced in the presence of Cur-MSNs compared to the control (untreated HN5 cells) (<i>p</i> < 0.05). A 3.43-fold increase in the <i>Bax/Bcl-2</i> ratio was seen in Cur-MSNs treated HN5 cells at the IC<sub>50</sub>. Cur-MSNs increased intracellular reactive oxygen species (ROS) production. Based on these novel results, we suggest that Cur-MSNs offer efficacy for cancer treatment and future studies should further characterize their properties in various experimental cancer models. |
| first_indexed | 2024-03-09T19:10:40Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T19:10:40Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-c37da4cfad9e4cae9a31b9ef5b40f0792023-11-24T04:13:00ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-10-0144115247525910.3390/cimb44110357Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5Simin Sharifi0Elaheh Dalir Abdolahinia1Mohammad Ali Ghavimi2Solmaz Maleki Dizaj3Michael Aschner4Luciano Saso5Haroon Khan6Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranResearch Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDepartment of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz 5166-15731, IranDepartment of Molecular Pharmacology, Albert Einstein College of Medicine Forchheimer, Bronx, NY 10461, USADepartment of Physiology and Pharmacology “Vittorio Erspamer”, Sapienza University, 00185 Rome, ItalyDepartment of Pharmacy, Abdul Wali Khan University Mardan, Mardan 23200, PakistanCurcumin is an active ingredient isolated from <i>Curcuma longa</i>. It has several pharmacological effects, including anticancer, anti-inflammatory, and antioxidant effects. Due to its low bioavailability, chemical structure instability, and easy oxidation, the application of curcumin has been limited. In this study, to overcome these limitations, curcumin-loaded mesoporous silica nanoparticles (Cur-MSN) were prepared, and the anticancerous effect of Cur-MSNs on head and neck cancer cells, HN5, was investigated. Transmission electron microscopy (TEM) revealed rod-shaped mesoporous nanoparticles with average particle size smaller than 100 nm. Higher cytotoxicity of Cur-MSNs was seen in treated cancer cells compared with free curcumin. The expression of <i>Bcl-2</i> was significantly reduced in the presence of Cur-MSNs compared to the control (untreated HN5 cells) (<i>p</i> < 0.05). A 3.43-fold increase in the <i>Bax/Bcl-2</i> ratio was seen in Cur-MSNs treated HN5 cells at the IC<sub>50</sub>. Cur-MSNs increased intracellular reactive oxygen species (ROS) production. Based on these novel results, we suggest that Cur-MSNs offer efficacy for cancer treatment and future studies should further characterize their properties in various experimental cancer models.https://www.mdpi.com/1467-3045/44/11/357curcuminmesoporous silica nanoparticlesanticancer<i>Bax/Bcl-2</i> |
| spellingShingle | Simin Sharifi Elaheh Dalir Abdolahinia Mohammad Ali Ghavimi Solmaz Maleki Dizaj Michael Aschner Luciano Saso Haroon Khan Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 Current Issues in Molecular Biology curcumin mesoporous silica nanoparticles anticancer <i>Bax/Bcl-2</i> |
| title | Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 |
| title_full | Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 |
| title_fullStr | Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 |
| title_full_unstemmed | Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 |
| title_short | Effect of Curcumin-Loaded Mesoporous Silica Nanoparticles on the Head and Neck Cancer Cell Line, HN5 |
| title_sort | effect of curcumin loaded mesoporous silica nanoparticles on the head and neck cancer cell line hn5 |
| topic | curcumin mesoporous silica nanoparticles anticancer <i>Bax/Bcl-2</i> |
| url | https://www.mdpi.com/1467-3045/44/11/357 |
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