Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway
Abstract Background Breast cancer is the most common cancer in women and affects 1.38 million women worldwide per year. Antiestrogens such as tamoxifen, a selective estrogen receptor (ER) modulator, are widely used in clinics to treat ER-positive breast tumors. However, remissions of breast cancer a...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-05-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2204-y |
| _version_ | 1819179486407557120 |
|---|---|
| author | Yu-Shih Lin Yin-Yin Lin Yao-Hsu Yang Chun-Liang Lin Feng-Che Kuan Cheng-Nan Lu Geng-He Chang Ming-Shao Tsai Cheng-Ming Hsu Reming-Albert Yeh Pei-Rung Yang I-Yun Lee Li-Hsin Shu Yu-Ching Cheng Hung-Te Liu Kuan-Der Lee De-Ching Chang Ching-Yuan Wu |
| author_facet | Yu-Shih Lin Yin-Yin Lin Yao-Hsu Yang Chun-Liang Lin Feng-Che Kuan Cheng-Nan Lu Geng-He Chang Ming-Shao Tsai Cheng-Ming Hsu Reming-Albert Yeh Pei-Rung Yang I-Yun Lee Li-Hsin Shu Yu-Ching Cheng Hung-Te Liu Kuan-Der Lee De-Ching Chang Ching-Yuan Wu |
| author_sort | Yu-Shih Lin |
| collection | DOAJ |
| description | Abstract Background Breast cancer is the most common cancer in women and affects 1.38 million women worldwide per year. Antiestrogens such as tamoxifen, a selective estrogen receptor (ER) modulator, are widely used in clinics to treat ER-positive breast tumors. However, remissions of breast cancer are often followed by resistance to tamoxifen and disease relapse. Despite the increasing understanding of the resistance mechanisms, effective regimens for treating tamoxifen-resistant breast cancer are limited. Antrodia cinnamomea is a traditional medicinal mushroom native only to Taiwan. In this study, we aimed to examine in vitro effect of antrodia cinnamomea in the tamoxifen-resistant cancer. Methods Antrodia cinnamomea was studied for its biological activity against proliferation of tamoxifen-resistant breast cancer by XTT assay. Next, the underlying mechanism was studied by flow cytometry, qPCR and Western’s blotting assay. Results Our results revealed that the ethanol extract of antrodia cinnamomea (AC) can inhibit the growth of breast cancer cells, including MCF-7 cell and tamoxifen-resistant MCF-7 cell lines. Combination treatment with AC and 10− 6 M tamoxifen have the better inhibitory effect on the proliferation of tamoxifen-resistant MCF-7 cells than only AC did. AC can induce apoptosis in these breast cancer cells. Moreover, it can suppress the mRNA expression of skp2 (S-phase kinase-associated protein 2) by increasing the expressions of miR-21-5p, miR-26-5p, and miR-30-5p in MCF-7 and tamoxifen-resistant MCF-7 cells. Conclusions These results suggest that the ethanol extract of antrodia cinnamomea could be a novel anticancer agent in the armamentarium of tamoxifen-resistant breast cancer management. Moreover, we hope to identify additional pure compounds that could serve as promising anti-breast cancer candidates for further clinical trials. |
| first_indexed | 2024-12-22T21:59:13Z |
| format | Article |
| id | doaj.art-c380bdcbbae4479eacc669e5d7ee163d |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T21:59:13Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-c380bdcbbae4479eacc669e5d7ee163d2022-12-21T18:11:11ZengBMCBMC Complementary and Alternative Medicine1472-68822018-05-0118111110.1186/s12906-018-2204-yAntrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathwayYu-Shih Lin0Yin-Yin Lin1Yao-Hsu Yang2Chun-Liang Lin3Feng-Che Kuan4Cheng-Nan Lu5Geng-He Chang6Ming-Shao Tsai7Cheng-Ming Hsu8Reming-Albert Yeh9Pei-Rung Yang10I-Yun Lee11Li-Hsin Shu12Yu-Ching Cheng13Hung-Te Liu14Kuan-Der Lee15De-Ching Chang16Ching-Yuan Wu17Department of Pharmacy, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartments of Nephrology, Chiayi Chang Gung Memorial HospitalDepartment of Hematology and oncology, Chiayi Chang Gung Memorial HospitalDivision of Acupuncture and Chinese Traumatology, Department of TCM, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Otolaryngology, Chang Gung Memorial HospitalDepartment of Otolaryngology, Chang Gung Memorial HospitalDepartment of Otolaryngology, Chang Gung Memorial HospitalDepartment of Otolaryngology, Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalDivision of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University HospitalInstitute of Molecular Biology, National Chung Cheng UniversityDepartment of Chinese Medicine, Chiayi Chang Gung Memorial HospitalAbstract Background Breast cancer is the most common cancer in women and affects 1.38 million women worldwide per year. Antiestrogens such as tamoxifen, a selective estrogen receptor (ER) modulator, are widely used in clinics to treat ER-positive breast tumors. However, remissions of breast cancer are often followed by resistance to tamoxifen and disease relapse. Despite the increasing understanding of the resistance mechanisms, effective regimens for treating tamoxifen-resistant breast cancer are limited. Antrodia cinnamomea is a traditional medicinal mushroom native only to Taiwan. In this study, we aimed to examine in vitro effect of antrodia cinnamomea in the tamoxifen-resistant cancer. Methods Antrodia cinnamomea was studied for its biological activity against proliferation of tamoxifen-resistant breast cancer by XTT assay. Next, the underlying mechanism was studied by flow cytometry, qPCR and Western’s blotting assay. Results Our results revealed that the ethanol extract of antrodia cinnamomea (AC) can inhibit the growth of breast cancer cells, including MCF-7 cell and tamoxifen-resistant MCF-7 cell lines. Combination treatment with AC and 10− 6 M tamoxifen have the better inhibitory effect on the proliferation of tamoxifen-resistant MCF-7 cells than only AC did. AC can induce apoptosis in these breast cancer cells. Moreover, it can suppress the mRNA expression of skp2 (S-phase kinase-associated protein 2) by increasing the expressions of miR-21-5p, miR-26-5p, and miR-30-5p in MCF-7 and tamoxifen-resistant MCF-7 cells. Conclusions These results suggest that the ethanol extract of antrodia cinnamomea could be a novel anticancer agent in the armamentarium of tamoxifen-resistant breast cancer management. Moreover, we hope to identify additional pure compounds that could serve as promising anti-breast cancer candidates for further clinical trials.http://link.springer.com/article/10.1186/s12906-018-2204-yBreast cancerTamoxifen-resistantskp2microRNAAntrodia cinnamomea |
| spellingShingle | Yu-Shih Lin Yin-Yin Lin Yao-Hsu Yang Chun-Liang Lin Feng-Che Kuan Cheng-Nan Lu Geng-He Chang Ming-Shao Tsai Cheng-Ming Hsu Reming-Albert Yeh Pei-Rung Yang I-Yun Lee Li-Hsin Shu Yu-Ching Cheng Hung-Te Liu Kuan-Der Lee De-Ching Chang Ching-Yuan Wu Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway BMC Complementary and Alternative Medicine Breast cancer Tamoxifen-resistant skp2 microRNA Antrodia cinnamomea |
| title | Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway |
| title_full | Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway |
| title_fullStr | Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway |
| title_full_unstemmed | Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway |
| title_short | Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway |
| title_sort | antrodia cinnamomea extract inhibits the proliferation of tamoxifen resistant breast cancer cells through apoptosis and skp2 micrornas pathway |
| topic | Breast cancer Tamoxifen-resistant skp2 microRNA Antrodia cinnamomea |
| url | http://link.springer.com/article/10.1186/s12906-018-2204-y |
| work_keys_str_mv | AT yushihlin antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT yinyinlin antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT yaohsuyang antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT chunlianglin antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT fengchekuan antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT chengnanlu antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT genghechang antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT mingshaotsai antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT chengminghsu antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT remingalbertyeh antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT peirungyang antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT iyunlee antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT lihsinshu antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT yuchingcheng antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT hungteliu antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT kuanderlee antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT dechingchang antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway AT chingyuanwu antrodiacinnamomeaextractinhibitstheproliferationoftamoxifenresistantbreastcancercellsthroughapoptosisandskp2micrornaspathway |