Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats
An experiment in broilers was conducted to investigate the effect of olive (Olea europea) leaves and marigold (Calendula officinalis) petal extract supplementation on oxidative stress, characteristics of intestinal contents, and on the morphology of the small intestine. Oxidative stress was induced...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2021-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/58/2/58_0200026/_html/-char/en |
| _version_ | 1797817080879251456 |
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| author | Tatjana Pirman Vida Rezar Milka Vrecl Janez Salobir Alenka Levart |
| author_facet | Tatjana Pirman Vida Rezar Milka Vrecl Janez Salobir Alenka Levart |
| author_sort | Tatjana Pirman |
| collection | DOAJ |
| description | An experiment in broilers was conducted to investigate the effect of olive (Olea europea) leaves and marigold (Calendula officinalis) petal extract supplementation on oxidative stress, characteristics of intestinal contents, and on the morphology of the small intestine. Oxidative stress was induced by a n-3 polyunsaturated fatty acids rich diet. 1-day-old male broiler chickens, Ross 308, were housed in a deep litter system. After the first 21 days, animals were randomly divided into three groups of 16 animals in two replicates and fed, until slaughter on day 39, a diet that contained 7% linseed oil. Control diet (Cont) remained unsupplemented, while both experimental diets were supplemented with olive leaves (OliveEx) or marigold petal (MarigEx) extracts. Oxidative stress was evaluated in blood and liver by measuring markers of lipid peroxidation (malondialdehyde (MDA), isoprostanes), rate of DNA damage in lymphocytes and in blood (comet assay, 8-hydroxy-2′deoxyguanosine (8-OHdG)), and activity of antioxidant and liver enzymes in blood. In different parts of the intestine, levels of short chain fatty acids (SCFA), and viscosity of intestinal contents were measured, and the health of the gastrointestinal tract was assessed using histological measurements. OliveEx significantly (p<0.05) decreased the MDA and 8-OHdG concentration in plasma, and the level of ethanoic acid in small intestinal contents and total SCFA in caecum, indicating improved oxidative status and increased microbial activity in the intestine. MarigEx significantly (p<0.05) decreased the rate of lymphocyte DNA damage and the crypt depth in duodenum, indicating potentially beneficial effects on the immune system and the health of the small intestine. In conclusion, dietary OliveEx and MarigEx supplementation improved some markers of oxidative stress and intestinal health. However, positive effects could be more pronounced in more unfavorable environmental conditions or in cases of diseases, but further studies are needed. |
| first_indexed | 2024-03-13T08:47:24Z |
| format | Article |
| id | doaj.art-c388b1e037a54293a73d5639b4cb1405 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:47:24Z |
| publishDate | 2021-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-c388b1e037a54293a73d5639b4cb14052023-05-30T01:00:10ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862021-04-0158211913010.2141/jpsa.0200026jpsaEffect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated FatsTatjana Pirman0Vida Rezar1Milka Vrecl2Janez Salobir3Alenka Levart4Department of Animal Science, Biotechnical Faculty, University of LjubljanaDepartment of Animal Science, Biotechnical Faculty, University of LjubljanaInstitute of Preclinical Sciences, Veterinary Faculty, University of LjubljanaDepartment of Animal Science, Biotechnical Faculty, University of LjubljanaDepartment of Animal Science, Biotechnical Faculty, University of LjubljanaAn experiment in broilers was conducted to investigate the effect of olive (Olea europea) leaves and marigold (Calendula officinalis) petal extract supplementation on oxidative stress, characteristics of intestinal contents, and on the morphology of the small intestine. Oxidative stress was induced by a n-3 polyunsaturated fatty acids rich diet. 1-day-old male broiler chickens, Ross 308, were housed in a deep litter system. After the first 21 days, animals were randomly divided into three groups of 16 animals in two replicates and fed, until slaughter on day 39, a diet that contained 7% linseed oil. Control diet (Cont) remained unsupplemented, while both experimental diets were supplemented with olive leaves (OliveEx) or marigold petal (MarigEx) extracts. Oxidative stress was evaluated in blood and liver by measuring markers of lipid peroxidation (malondialdehyde (MDA), isoprostanes), rate of DNA damage in lymphocytes and in blood (comet assay, 8-hydroxy-2′deoxyguanosine (8-OHdG)), and activity of antioxidant and liver enzymes in blood. In different parts of the intestine, levels of short chain fatty acids (SCFA), and viscosity of intestinal contents were measured, and the health of the gastrointestinal tract was assessed using histological measurements. OliveEx significantly (p<0.05) decreased the MDA and 8-OHdG concentration in plasma, and the level of ethanoic acid in small intestinal contents and total SCFA in caecum, indicating improved oxidative status and increased microbial activity in the intestine. MarigEx significantly (p<0.05) decreased the rate of lymphocyte DNA damage and the crypt depth in duodenum, indicating potentially beneficial effects on the immune system and the health of the small intestine. In conclusion, dietary OliveEx and MarigEx supplementation improved some markers of oxidative stress and intestinal health. However, positive effects could be more pronounced in more unfavorable environmental conditions or in cases of diseases, but further studies are needed.https://www.jstage.jst.go.jp/article/jpsa/58/2/58_0200026/_html/-char/enbroiler chickenintestine morphologymarigoldolive leavesoxidative stressshort chain fatty acids |
| spellingShingle | Tatjana Pirman Vida Rezar Milka Vrecl Janez Salobir Alenka Levart Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats The Journal of Poultry Science broiler chicken intestine morphology marigold olive leaves oxidative stress short chain fatty acids |
| title | Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats |
| title_full | Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats |
| title_fullStr | Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats |
| title_full_unstemmed | Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats |
| title_short | Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats |
| title_sort | effect of olive leaves or marigold petal extract on oxidative stress gut fermentative activity and mucosa morphology in broiler chickens fed a diet rich in n 3 polyunsaturated fats |
| topic | broiler chicken intestine morphology marigold olive leaves oxidative stress short chain fatty acids |
| url | https://www.jstage.jst.go.jp/article/jpsa/58/2/58_0200026/_html/-char/en |
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