Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet
Mice fed a high fat diet (HFD) ab libitum show corneal dysregulation, as evidenced by decreased sensitivity and impaired wound healing. Time-restricted (TR) feeding can effectively mitigate the cardiometabolic effects of an HFD. To determine if TR feeding attenuates HFD-induced corneal dysregulation...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/1/139 |
| _version_ | 1797498010970619904 |
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| author | Prince K. Akowuah Aubrey Hargrave Rolando E. Rumbaut Alan R. Burns |
| author_facet | Prince K. Akowuah Aubrey Hargrave Rolando E. Rumbaut Alan R. Burns |
| author_sort | Prince K. Akowuah |
| collection | DOAJ |
| description | Mice fed a high fat diet (HFD) ab libitum show corneal dysregulation, as evidenced by decreased sensitivity and impaired wound healing. Time-restricted (TR) feeding can effectively mitigate the cardiometabolic effects of an HFD. To determine if TR feeding attenuates HFD-induced corneal dysregulation, this study evaluated 6-week-old C57BL/6 mice fed an ad libitum normal diet (ND), an ad libitum HFD, or a time-restricted (TR) HFD for 10 days. Corneal sensitivity was measured using a Cochet-Bonnet aesthesiometer. A corneal epithelial abrasion wound was created, and wound closure was monitored for 30 h. Neutrophil and platelet recruitment were assessed by immunofluorescence microscopy. TR HFD fed mice gained less weight (<i>p</i> < 0.0001), had less visceral fat (<i>p</i> = 0.015), and had reduced numbers of adipose tissue macrophages and T cells (<i>p</i> < 0.05) compared to ad libitum HFD fed mice. Corneal sensitivity was reduced in ad libitum HFD and TR HFD fed mice compared to ad libitum ND fed mice (<i>p</i> < 0.0001). Following epithelial abrasion, corneal wound closure was delayed (~6 h), and neutrophil and platelet recruitment was dysregulated similarly in ad libitum and TR HFD fed mice. TR HFD feeding appears to mitigate adipose tissue inflammation and adiposity, while the cornea remains sensitive to the pathologic effects of HFD feeding. |
| first_indexed | 2024-03-10T03:27:20Z |
| format | Article |
| id | doaj.art-c38b5cc88d264edc949ffb1ae2a8f2f3 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T03:27:20Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c38b5cc88d264edc949ffb1ae2a8f2f32023-11-23T12:04:52ZengMDPI AGNutrients2072-66432021-12-0114113910.3390/nu14010139Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat DietPrince K. Akowuah0Aubrey Hargrave1Rolando E. Rumbaut2Alan R. Burns3College of Optometry, University of Houston, Houston, TX 77204, USACollege of Optometry, University of Houston, Houston, TX 77204, USAChildren’s Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030, USACollege of Optometry, University of Houston, Houston, TX 77204, USAMice fed a high fat diet (HFD) ab libitum show corneal dysregulation, as evidenced by decreased sensitivity and impaired wound healing. Time-restricted (TR) feeding can effectively mitigate the cardiometabolic effects of an HFD. To determine if TR feeding attenuates HFD-induced corneal dysregulation, this study evaluated 6-week-old C57BL/6 mice fed an ad libitum normal diet (ND), an ad libitum HFD, or a time-restricted (TR) HFD for 10 days. Corneal sensitivity was measured using a Cochet-Bonnet aesthesiometer. A corneal epithelial abrasion wound was created, and wound closure was monitored for 30 h. Neutrophil and platelet recruitment were assessed by immunofluorescence microscopy. TR HFD fed mice gained less weight (<i>p</i> < 0.0001), had less visceral fat (<i>p</i> = 0.015), and had reduced numbers of adipose tissue macrophages and T cells (<i>p</i> < 0.05) compared to ad libitum HFD fed mice. Corneal sensitivity was reduced in ad libitum HFD and TR HFD fed mice compared to ad libitum ND fed mice (<i>p</i> < 0.0001). Following epithelial abrasion, corneal wound closure was delayed (~6 h), and neutrophil and platelet recruitment was dysregulated similarly in ad libitum and TR HFD fed mice. TR HFD feeding appears to mitigate adipose tissue inflammation and adiposity, while the cornea remains sensitive to the pathologic effects of HFD feeding.https://www.mdpi.com/2072-6643/14/1/139corneal dysregulationhigh fat dietobesityneutrophilsplateletstime-restricted feeding |
| spellingShingle | Prince K. Akowuah Aubrey Hargrave Rolando E. Rumbaut Alan R. Burns Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet Nutrients corneal dysregulation high fat diet obesity neutrophils platelets time-restricted feeding |
| title | Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet |
| title_full | Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet |
| title_fullStr | Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet |
| title_full_unstemmed | Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet |
| title_short | Dissociation between Corneal and Cardiometabolic Changes in Response to a Time-Restricted Feeding of a High Fat Diet |
| title_sort | dissociation between corneal and cardiometabolic changes in response to a time restricted feeding of a high fat diet |
| topic | corneal dysregulation high fat diet obesity neutrophils platelets time-restricted feeding |
| url | https://www.mdpi.com/2072-6643/14/1/139 |
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