Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots
Abstract Small RNAs comprise three families of noncoding regulatory RNAs that control gene expression by blocking mRNA translation or leading to mRNA cleavage. Such post‐transcriptional negative regulation is relevant for both plant development and environmental adaptations. An important biotechnolo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-03-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.204 |
| _version_ | 1819134583964172288 |
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| author | Eveline Queiroz de Pinho Tavares Marina Camara Mattos Martins Adriana Grandis Grayce H. Romim Amanda Rusiska Piovezani Jonas Weissmann Gaiarsa Marcos Silveira Buckeridge |
| author_facet | Eveline Queiroz de Pinho Tavares Marina Camara Mattos Martins Adriana Grandis Grayce H. Romim Amanda Rusiska Piovezani Jonas Weissmann Gaiarsa Marcos Silveira Buckeridge |
| author_sort | Eveline Queiroz de Pinho Tavares |
| collection | DOAJ |
| description | Abstract Small RNAs comprise three families of noncoding regulatory RNAs that control gene expression by blocking mRNA translation or leading to mRNA cleavage. Such post‐transcriptional negative regulation is relevant for both plant development and environmental adaptations. An important biotechnological application of miRNA identification is the discovery of regulators and effectors of cell wall degradation, which can improve/facilitate hydrolysis of cell wall polymers for second‐generation bioethanol production. The recent characterization of plant innate cell wall modifications occurring during root aerenchyma development triggered by ethylene led to the possibility of prospection for mechanisms of cell wall disassembly in sugarcane. By using next‐generation sequencing, 39 miRNAs were identified in root segments along the process of aerenchyma development. Among them, 31 miRNAs were unknown to the sugarcane miRBase repository but previously identified as produced by its relative Sorghum bicolor. Key putative targets related to signal transduction, carbohydrate metabolic process, and cell wall organization or biogenesis were among the most representative gene categories targeted by miRNA. They belong to the subclasses of genes associated with the four modules of cell wall modification in sugarcane roots: cell expansion, cell separation, hemicellulose, and cellulose hydrolysis. Thirteen miRNAs possibly related to ethylene perception and signaling were also identified. Our findings suggest that miRNAs may be involved in the regulation of cell wall degradation during aerenchyma formation. This work also points out to potential molecular tools for sugarcane improvement in the context of second‐generation biofuels. |
| first_indexed | 2024-12-22T10:05:30Z |
| format | Article |
| id | doaj.art-c38bf2bdfb4b429eb79f6a4bac9c67b1 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-22T10:05:30Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-c38bf2bdfb4b429eb79f6a4bac9c67b12022-12-21T18:29:59ZengWileyPlant Direct2475-44552020-03-0143n/an/a10.1002/pld3.204Newly identified miRNAs may contribute to aerenchyma formation in sugarcane rootsEveline Queiroz de Pinho Tavares0Marina Camara Mattos Martins1Adriana Grandis2Grayce H. Romim3Amanda Rusiska Piovezani4Jonas Weissmann Gaiarsa5Marcos Silveira Buckeridge6Departamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilDepartamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilDepartamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilDepartamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilDepartamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilCentro de Facilidades Para a Pesquisa Instituto de Ciências Biomédicas Universidade de São Paulo São Paulo BrazilDepartamento de Botânica Instituto de Biociências Universidade de São Paulo São Paulo BrazilAbstract Small RNAs comprise three families of noncoding regulatory RNAs that control gene expression by blocking mRNA translation or leading to mRNA cleavage. Such post‐transcriptional negative regulation is relevant for both plant development and environmental adaptations. An important biotechnological application of miRNA identification is the discovery of regulators and effectors of cell wall degradation, which can improve/facilitate hydrolysis of cell wall polymers for second‐generation bioethanol production. The recent characterization of plant innate cell wall modifications occurring during root aerenchyma development triggered by ethylene led to the possibility of prospection for mechanisms of cell wall disassembly in sugarcane. By using next‐generation sequencing, 39 miRNAs were identified in root segments along the process of aerenchyma development. Among them, 31 miRNAs were unknown to the sugarcane miRBase repository but previously identified as produced by its relative Sorghum bicolor. Key putative targets related to signal transduction, carbohydrate metabolic process, and cell wall organization or biogenesis were among the most representative gene categories targeted by miRNA. They belong to the subclasses of genes associated with the four modules of cell wall modification in sugarcane roots: cell expansion, cell separation, hemicellulose, and cellulose hydrolysis. Thirteen miRNAs possibly related to ethylene perception and signaling were also identified. Our findings suggest that miRNAs may be involved in the regulation of cell wall degradation during aerenchyma formation. This work also points out to potential molecular tools for sugarcane improvement in the context of second‐generation biofuels.https://doi.org/10.1002/pld3.204aerenchymabioenergycell wallmiRNAsugarcane |
| spellingShingle | Eveline Queiroz de Pinho Tavares Marina Camara Mattos Martins Adriana Grandis Grayce H. Romim Amanda Rusiska Piovezani Jonas Weissmann Gaiarsa Marcos Silveira Buckeridge Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots Plant Direct aerenchyma bioenergy cell wall miRNA sugarcane |
| title | Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots |
| title_full | Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots |
| title_fullStr | Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots |
| title_full_unstemmed | Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots |
| title_short | Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots |
| title_sort | newly identified mirnas may contribute to aerenchyma formation in sugarcane roots |
| topic | aerenchyma bioenergy cell wall miRNA sugarcane |
| url | https://doi.org/10.1002/pld3.204 |
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