| Summary: | <i>Anastatus japonicus</i> Ashmead is a widely used biological control agent against stink bugs that can be successfully reared using the large eggs of the Chinese silkworm. In this study, environmental factors responsible for the induction of diapause in <i>A. japonicus</i> were investigated on host eggs of the Chinese silkworm. <i>A. japonicus</i> exhibited a facultative, mature larval diapause within its host eggs. Second–third instar larva are the most sensitive stages to diapause stimuli. The accumulation of diapause stimuli during all the larval stages maximized the diapause response. A short photoperiod of 10L:14D and temperature of 17 °C led to the occurrence of the highest diapause response, while a long photoperiod (14L:10D) and low temperatures (11 and 14 °C) prevented the diapause. A specific exposure period was required to reach high diapause incidence. Diapausing mature larvae had a significantly higher survival rate after 180 days storage at 10 °C than that of nondiapausing mature larvae. Taken together, results suggest methods that could be exploited in the developmental regulation, field-release pretreatment technology, and long-term storage of <i>A. japonicus</i>.
|
|---|