Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina
Background Diadumene lineata is one of the most widespread sea anemone species worldwide. Although this species has been reported a few times on the Argentine coast since 2004, its identification has traditionally been based on external morphological characteristics, and in most cases no voucher spe...
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PeerJ Inc.
2023-11-01
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| author | Ricardo González Muñoz Daniel Lauretta María Cielo Bazterrica Francisco Alejandro Puente Tapia Agustín Garese Gregorio Bigatti Pablo E. Penchaszadeh Betina Lomovasky Fabián H. Acuña |
| author_facet | Ricardo González Muñoz Daniel Lauretta María Cielo Bazterrica Francisco Alejandro Puente Tapia Agustín Garese Gregorio Bigatti Pablo E. Penchaszadeh Betina Lomovasky Fabián H. Acuña |
| author_sort | Ricardo González Muñoz |
| collection | DOAJ |
| description | Background Diadumene lineata is one of the most widespread sea anemone species worldwide. Although this species has been reported a few times on the Argentine coast since 2004, its identification has traditionally been based on external morphological characteristics, and in most cases no voucher specimens are available to support previous records. Methods In this study, we obtained DNA sequences of two mitochondrial markers (12S and 16S) and two nuclear markers (18S and 28S) from specimens of D. lineata collected in two locations on the Argentine coast separated by almost 800 km. Additionally, we conducted an analysis of the morphology, as well as the types and size ranges of cnidae, using specimens collected at three different locations along the Argentine coast. Furthermore, since introduced populations of D. lineata are presumably ephemeral and only reproduce asexually outside their native range, we examined the internal anatomy of representatives from the Argentine coast for gametogenic tissue as an indication of whether they might be capable of sexual reproduction. Results DNA data support our morphological identification, including cnidae analyses, of the specimens as D. lineata. Furthermore, all specimens examined were determined to be sterile. Discussion Genetic sequence comparisons, phylogenetic reconstruction, and cnidae data support the identification of individuals of D. lineata from Mar Chiquita and Garipe Beach, confirming the presence of the species on the Argentine coast using both morphological and molecular tools. The absence of fertile specimens suggests that each sampled population is likely reproducing only by asexual reproduction and possibly composed of clones. The presence of an additional category of longer p-mastigophores B2a in the actinopharynx and filaments, as well as holotrichs in the column, is also reported. Conclusions For the first time, we have confirmed the presence of D. lineata in Argentina through molecular data. Additionally, our findings indicate that the analyzed specimens are sterile, suggesting that this species is not engaging in sexual reproduction in the studied localities. It is crucial to continue monitoring the populations of D. lineata along the Argentine coast to assess whether they establish sexual reproduction, expand their distribution range or disappear, or potentially cause any harm to local species or alterations in benthic communities. |
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| spelling | doaj.art-c3929e669fa04477959677b8e0c4d73c2023-11-29T15:05:18ZengPeerJ Inc.PeerJ2167-83592023-11-0111e1647910.7717/peerj.16479Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in ArgentinaRicardo González Muñoz0Daniel Lauretta1María Cielo Bazterrica2Francisco Alejandro Puente Tapia3Agustín Garese4Gregorio Bigatti5Pablo E. Penchaszadeh6Betina Lomovasky7Fabián H. Acuña8Laboratorio de Biología de Cnidarios, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Buenos Aires, ArgentinaLaboratorio de Ecosistemas Costeros, Plataforma y Mar Profundo—Malacología, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”, CONICET, Ciudad Autónoma de Buenos Aires, ArgentinaInstituto de Investigaciones Marinas y Costeras (IIMyC-CONICET/UNMdP), Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones Marinas y Costeras (IIMyC-CONICET/UNMdP), Mar del Plata, Buenos Aires, ArgentinaLaboratorio de Biología de Cnidarios, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Buenos Aires, ArgentinaLaboratorio de Reproducción y Biología Integrativa de Invertebrados Marinos, (LARBIM, IBIOMAR), CONICET, Puerto Madryn, Chubut, ArgentinaLaboratorio de Ecosistemas Costeros, Plataforma y Mar Profundo—Malacología, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”, CONICET, Ciudad Autónoma de Buenos Aires, ArgentinaInstituto de Investigaciones Marinas y Costeras (IIMyC-CONICET/UNMdP), Mar del Plata, Buenos Aires, ArgentinaLaboratorio de Biología de Cnidarios, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Buenos Aires, ArgentinaBackground Diadumene lineata is one of the most widespread sea anemone species worldwide. Although this species has been reported a few times on the Argentine coast since 2004, its identification has traditionally been based on external morphological characteristics, and in most cases no voucher specimens are available to support previous records. Methods In this study, we obtained DNA sequences of two mitochondrial markers (12S and 16S) and two nuclear markers (18S and 28S) from specimens of D. lineata collected in two locations on the Argentine coast separated by almost 800 km. Additionally, we conducted an analysis of the morphology, as well as the types and size ranges of cnidae, using specimens collected at three different locations along the Argentine coast. Furthermore, since introduced populations of D. lineata are presumably ephemeral and only reproduce asexually outside their native range, we examined the internal anatomy of representatives from the Argentine coast for gametogenic tissue as an indication of whether they might be capable of sexual reproduction. Results DNA data support our morphological identification, including cnidae analyses, of the specimens as D. lineata. Furthermore, all specimens examined were determined to be sterile. Discussion Genetic sequence comparisons, phylogenetic reconstruction, and cnidae data support the identification of individuals of D. lineata from Mar Chiquita and Garipe Beach, confirming the presence of the species on the Argentine coast using both morphological and molecular tools. The absence of fertile specimens suggests that each sampled population is likely reproducing only by asexual reproduction and possibly composed of clones. The presence of an additional category of longer p-mastigophores B2a in the actinopharynx and filaments, as well as holotrichs in the column, is also reported. Conclusions For the first time, we have confirmed the presence of D. lineata in Argentina through molecular data. Additionally, our findings indicate that the analyzed specimens are sterile, suggesting that this species is not engaging in sexual reproduction in the studied localities. It is crucial to continue monitoring the populations of D. lineata along the Argentine coast to assess whether they establish sexual reproduction, expand their distribution range or disappear, or potentially cause any harm to local species or alterations in benthic communities.https://peerj.com/articles/16479.pdfArgentinian coastAsexual reproductionBenthic intertidal faunaMarine invasive invertebratesMitochondrial markersNuclear markers |
| spellingShingle | Ricardo González Muñoz Daniel Lauretta María Cielo Bazterrica Francisco Alejandro Puente Tapia Agustín Garese Gregorio Bigatti Pablo E. Penchaszadeh Betina Lomovasky Fabián H. Acuña Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina PeerJ Argentinian coast Asexual reproduction Benthic intertidal fauna Marine invasive invertebrates Mitochondrial markers Nuclear markers |
| title | Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina |
| title_full | Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina |
| title_fullStr | Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina |
| title_full_unstemmed | Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina |
| title_short | Mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone Diadumene lineata (Verrill, 1869) (Cnidaria: Actiniaria) in Argentina |
| title_sort | mitochondrial and nuclear gene sequencing confirms the presence of the invasive sea anemone diadumene lineata verrill 1869 cnidaria actiniaria in argentina |
| topic | Argentinian coast Asexual reproduction Benthic intertidal fauna Marine invasive invertebrates Mitochondrial markers Nuclear markers |
| url | https://peerj.com/articles/16479.pdf |
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