“Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells
The effect of Bacitracin as an antibiotic acting against Gram-positive bacterial cells was evaluated in combination with hexahistidine-containing organophosphate hydrolase (His<sub>6</sub>-OPH), possessing lactonase activity against various <i>N</i>-acylhomoserine lactones pr...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Aysel Aslanli Maksim Domnin Nikolay Stepanov Elena Efremenko |
| author_facet | Aysel Aslanli Maksim Domnin Nikolay Stepanov Elena Efremenko |
| author_sort | Aysel Aslanli |
| collection | DOAJ |
| description | The effect of Bacitracin as an antibiotic acting against Gram-positive bacterial cells was evaluated in combination with hexahistidine-containing organophosphate hydrolase (His<sub>6</sub>-OPH), possessing lactonase activity against various <i>N</i>-acylhomoserine lactones produced by most Gram-negative bacteria as quorum-sensing molecules. The molecular docking technique was used to obtain in silico confirmation of possible interactions between molecules of His<sub>6</sub>-OPH and Bacitracin as well as the absence of a significant influence of such interactions on the enzymatic catalysis. The in vitro experiments showed a sufficient catalytic efficiency of action of the His<sub>6</sub>-OPH/Bacitracin combination as compared to the native enzyme. The notable improvement (up to 3.3 times) of antibacterial efficiency of Bacitracin was revealed in relation to Gram-negative bacteria when it was used in combination with His<sub>6</sub>-OPH. For the first time, the action of the Bacitracin with and without His<sub>6</sub>-OPH was shown to be effective against various yeast strains, and the presence of the enzyme increased the antibiotic effect up to 8.5 times. To estimate the role of the enzyme in the success of His<sub>6</sub>-OPH/Bacitracin with yeast, in silico experiments (molecular docking) with various fungous lactone-containing molecules were undertaken, and the opportunity of their enzymatic hydrolysis by His<sub>6</sub>-OPH was revealed in the presence and absence of Bacitracin. |
| first_indexed | 2024-03-09T09:55:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:55:43Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c393beb62dc2448d8fa43dc58e65b9522023-12-01T23:48:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316940010.3390/ijms23169400“Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast CellsAysel Aslanli0Maksim Domnin1Nikolay Stepanov2Elena Efremenko3Chemical Faculty, Lomonosov Moscow State University, Lenin Hills 1/3, 119991 Moscow, RussiaChemical Faculty, Lomonosov Moscow State University, Lenin Hills 1/3, 119991 Moscow, RussiaChemical Faculty, Lomonosov Moscow State University, Lenin Hills 1/3, 119991 Moscow, RussiaChemical Faculty, Lomonosov Moscow State University, Lenin Hills 1/3, 119991 Moscow, RussiaThe effect of Bacitracin as an antibiotic acting against Gram-positive bacterial cells was evaluated in combination with hexahistidine-containing organophosphate hydrolase (His<sub>6</sub>-OPH), possessing lactonase activity against various <i>N</i>-acylhomoserine lactones produced by most Gram-negative bacteria as quorum-sensing molecules. The molecular docking technique was used to obtain in silico confirmation of possible interactions between molecules of His<sub>6</sub>-OPH and Bacitracin as well as the absence of a significant influence of such interactions on the enzymatic catalysis. The in vitro experiments showed a sufficient catalytic efficiency of action of the His<sub>6</sub>-OPH/Bacitracin combination as compared to the native enzyme. The notable improvement (up to 3.3 times) of antibacterial efficiency of Bacitracin was revealed in relation to Gram-negative bacteria when it was used in combination with His<sub>6</sub>-OPH. For the first time, the action of the Bacitracin with and without His<sub>6</sub>-OPH was shown to be effective against various yeast strains, and the presence of the enzyme increased the antibiotic effect up to 8.5 times. To estimate the role of the enzyme in the success of His<sub>6</sub>-OPH/Bacitracin with yeast, in silico experiments (molecular docking) with various fungous lactone-containing molecules were undertaken, and the opportunity of their enzymatic hydrolysis by His<sub>6</sub>-OPH was revealed in the presence and absence of Bacitracin.https://www.mdpi.com/1422-0067/23/16/9400bacitracinhexahistidine-containing organophosphorus hydrolasecombinationmolecular dockingquorum quenchingbacteria |
| spellingShingle | Aysel Aslanli Maksim Domnin Nikolay Stepanov Elena Efremenko “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells International Journal of Molecular Sciences bacitracin hexahistidine-containing organophosphorus hydrolase combination molecular docking quorum quenching bacteria |
| title | “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells |
| title_full | “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells |
| title_fullStr | “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells |
| title_full_unstemmed | “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells |
| title_short | “Universal” Antimicrobial Combination of Bacitracin and His<sub>6</sub>-OPH with Lactonase Activity, Acting against Various Bacterial and Yeast Cells |
| title_sort | universal antimicrobial combination of bacitracin and his sub 6 sub oph with lactonase activity acting against various bacterial and yeast cells |
| topic | bacitracin hexahistidine-containing organophosphorus hydrolase combination molecular docking quorum quenching bacteria |
| url | https://www.mdpi.com/1422-0067/23/16/9400 |
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