Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism
Summary: A variety of anthropogenic organohalide contaminants generated from industry are released into the environment and thus cause serious pollution that endangers human health. In the present study, we investigated the microbial community composition of industrial saponification wastewater usin...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | The Innovation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666675822001515 |
| _version_ | 1811187607219994624 |
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| author | Yiqun Huang Lingyu Wen Lige Zhang Jijun Xu Weiwei Wang Haiyang Hu Ping Xu Zhao Li Hongzhi Tang |
| author_facet | Yiqun Huang Lingyu Wen Lige Zhang Jijun Xu Weiwei Wang Haiyang Hu Ping Xu Zhao Li Hongzhi Tang |
| author_sort | Yiqun Huang |
| collection | DOAJ |
| description | Summary: A variety of anthropogenic organohalide contaminants generated from industry are released into the environment and thus cause serious pollution that endangers human health. In the present study, we investigated the microbial community composition of industrial saponification wastewater using 16S rRNA sequencing, providing genomic insights of potential organohalide dehalogenation bacteria (OHDBs) by metagenomic sequencing. We also explored yet-to-culture OHDBs involved in the microbial community. Microbial diversity analysis reveals that Proteobacteria and Patescibacteria phyla dominate microbiome abundance of the wastewater. In addition, a total of six bacterial groups (Rhizobiales, Rhodobacteraceae, Rhodospirillales, Flavobacteriales, Micrococcales, and Saccharimonadales) were found as biomarkers in the key organohalide removal module. Ninety-four metagenome-assembled genomes were reconstructed from the microbial community, and 105 hydrolytic dehalogenase genes within 42 metagenome-assembled genomes were identified, suggesting that the potential for organohalide hydrolytic dehalogenation is present in the microbial community. Subsequently, we characterized the organohalide dehalogenation of an isolated OHDB, Microbacterium sp. J1-1, which shows the dehalogenation activities of chloropropanol, dichloropropanol, and epichlorohydrin. This study provides a community-integrated multi-omics approach to gain functional OHDBs for industrial organohalide dehalogenation. |
| first_indexed | 2024-04-11T14:06:15Z |
| format | Article |
| id | doaj.art-c39571db7abd4723ad34653d22af5070 |
| institution | Directory Open Access Journal |
| issn | 2666-6758 |
| language | English |
| last_indexed | 2024-04-11T14:06:15Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Innovation |
| spelling | doaj.art-c39571db7abd4723ad34653d22af50702022-12-22T04:19:53ZengElsevierThe Innovation2666-67582023-01-0141100355Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganismYiqun Huang0Lingyu Wen1Lige Zhang2Jijun Xu3Weiwei Wang4Haiyang Hu5Ping Xu6Zhao Li7Hongzhi Tang8State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaBefar Group Co., Ltd., Binzhou, Shandong 256619, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaBefar Group Co., Ltd., Binzhou, Shandong 256619, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding authorSummary: A variety of anthropogenic organohalide contaminants generated from industry are released into the environment and thus cause serious pollution that endangers human health. In the present study, we investigated the microbial community composition of industrial saponification wastewater using 16S rRNA sequencing, providing genomic insights of potential organohalide dehalogenation bacteria (OHDBs) by metagenomic sequencing. We also explored yet-to-culture OHDBs involved in the microbial community. Microbial diversity analysis reveals that Proteobacteria and Patescibacteria phyla dominate microbiome abundance of the wastewater. In addition, a total of six bacterial groups (Rhizobiales, Rhodobacteraceae, Rhodospirillales, Flavobacteriales, Micrococcales, and Saccharimonadales) were found as biomarkers in the key organohalide removal module. Ninety-four metagenome-assembled genomes were reconstructed from the microbial community, and 105 hydrolytic dehalogenase genes within 42 metagenome-assembled genomes were identified, suggesting that the potential for organohalide hydrolytic dehalogenation is present in the microbial community. Subsequently, we characterized the organohalide dehalogenation of an isolated OHDB, Microbacterium sp. J1-1, which shows the dehalogenation activities of chloropropanol, dichloropropanol, and epichlorohydrin. This study provides a community-integrated multi-omics approach to gain functional OHDBs for industrial organohalide dehalogenation.http://www.sciencedirect.com/science/article/pii/S2666675822001515organohalidewastewatermetagenome assembled genomemicrobial community |
| spellingShingle | Yiqun Huang Lingyu Wen Lige Zhang Jijun Xu Weiwei Wang Haiyang Hu Ping Xu Zhao Li Hongzhi Tang Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism The Innovation organohalide wastewater metagenome assembled genome microbial community |
| title | Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| title_full | Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| title_fullStr | Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| title_full_unstemmed | Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| title_short | Community-integrated multi-omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| title_sort | community integrated multi omics facilitates screening and isolation of the organohalide dehalogenation microorganism |
| topic | organohalide wastewater metagenome assembled genome microbial community |
| url | http://www.sciencedirect.com/science/article/pii/S2666675822001515 |
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