The Effect of Vitrification on Follicular Morphology of Ovarian Rat
Background & Objective: Some efforts have been made for keeping cryopreservation of gametes and embryos safe, including new vitrification methods of the ovary. This study evaluates the effect of ethylene glycole vitrification on follicular morphology of ovarian rat. Materials & Methods: Eig...
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2015-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
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| Online Access: | http://jabs.fums.ac.ir/article-1-608-en.pdf |
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| author | Foroozan Esmaeilzadeh Laleh Hamedi Davood Mehrabani Aalamtaj Bidshahri Nader Tanideh |
| author_facet | Foroozan Esmaeilzadeh Laleh Hamedi Davood Mehrabani Aalamtaj Bidshahri Nader Tanideh |
| author_sort | Foroozan Esmaeilzadeh |
| collection | DOAJ |
| description | Background & Objective: Some efforts have been made for keeping cryopreservation of gametes and embryos safe, including new vitrification methods of the ovary. This study evaluates the effect of ethylene glycole vitrification on follicular morphology of ovarian rat.
Materials & Methods: Eighty ovaries belonging to 40 rats are divided into 2 groups. Twenty five ovaries are control group, 25 the vitrification, and30 toxicologic effects. For freezing, equilibrium solution, ethylene glycole and methyl sulfoxide are used. For defreezeing, different concentrations of saccharose and for morphological evaluation, H&E staining are undertaken. The number of healthy and atretic follicles are determined after 24 hours, 1 week and one month after vitrification.
Results: No morphological changes are observed in all follicular cells. The percent of primordial, primary, secondary, anthral and developed follicles in the vitrification group are 34.5%, 17.7%, 17.4%, 15.2% and 50.3%. In vitrification and toxicological groups, the percent of both normal and atretic follicles is 47.5% and 11.9%. These figures for the control group were 59.7% and 16.9%. In vitrification method, 91% of oocytes are viable, 81% have mitosis, and 50% enters blastocyst stage.
Conclusion: Because in vitrification of ovary in comparison with the follicles, many types of follicles in different cycles can be recovered with no morphological and structural changes, vitrification of ovary can be a safe method for cryopreservation of the oocytes |
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| format | Article |
| id | doaj.art-c398b9fa7b1e45ed8bacbf48992d0848 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:54Z |
| publishDate | 2015-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-c398b9fa7b1e45ed8bacbf48992d08482023-09-20T21:20:19ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232015-08-0152185195The Effect of Vitrification on Follicular Morphology of Ovarian RatForoozan Esmaeilzadeh0Laleh Hamedi1Davood Mehrabani2Aalamtaj Bidshahri3Nader Tanideh4 Department of Chemistry, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran Department of Biology, Islamic Azad University, Jahrom Branch Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran Department of Chemistry, Islamic Azad University, Marvdasht Branch Background & Objective: Some efforts have been made for keeping cryopreservation of gametes and embryos safe, including new vitrification methods of the ovary. This study evaluates the effect of ethylene glycole vitrification on follicular morphology of ovarian rat. Materials & Methods: Eighty ovaries belonging to 40 rats are divided into 2 groups. Twenty five ovaries are control group, 25 the vitrification, and30 toxicologic effects. For freezing, equilibrium solution, ethylene glycole and methyl sulfoxide are used. For defreezeing, different concentrations of saccharose and for morphological evaluation, H&E staining are undertaken. The number of healthy and atretic follicles are determined after 24 hours, 1 week and one month after vitrification. Results: No morphological changes are observed in all follicular cells. The percent of primordial, primary, secondary, anthral and developed follicles in the vitrification group are 34.5%, 17.7%, 17.4%, 15.2% and 50.3%. In vitrification and toxicological groups, the percent of both normal and atretic follicles is 47.5% and 11.9%. These figures for the control group were 59.7% and 16.9%. In vitrification method, 91% of oocytes are viable, 81% have mitosis, and 50% enters blastocyst stage. Conclusion: Because in vitrification of ovary in comparison with the follicles, many types of follicles in different cycles can be recovered with no morphological and structural changes, vitrification of ovary can be a safe method for cryopreservation of the oocyteshttp://jabs.fums.ac.ir/article-1-608-en.pdfvitrificationfollicleoocyteovarymorphologyrat |
| spellingShingle | Foroozan Esmaeilzadeh Laleh Hamedi Davood Mehrabani Aalamtaj Bidshahri Nader Tanideh The Effect of Vitrification on Follicular Morphology of Ovarian Rat Journal of Advanced Biomedical Sciences vitrification follicle oocyte ovary morphology rat |
| title | The Effect of Vitrification on Follicular Morphology of Ovarian Rat |
| title_full | The Effect of Vitrification on Follicular Morphology of Ovarian Rat |
| title_fullStr | The Effect of Vitrification on Follicular Morphology of Ovarian Rat |
| title_full_unstemmed | The Effect of Vitrification on Follicular Morphology of Ovarian Rat |
| title_short | The Effect of Vitrification on Follicular Morphology of Ovarian Rat |
| title_sort | effect of vitrification on follicular morphology of ovarian rat |
| topic | vitrification follicle oocyte ovary morphology rat |
| url | http://jabs.fums.ac.ir/article-1-608-en.pdf |
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