Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models
Objective: Several species of Pinus have shown anti-nociceptive and anti-inflammatory properties. This study was aimed to evaluate anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract of bark and essential oil of leaves of P. eldarica in animal models. Materials and Methods: Hyd...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2021-09-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | https://ajp.mums.ac.ir/article_18038_d6f5e6462a6a90df615c8c4072d75fec.pdf |
| _version_ | 1818681788250193920 |
|---|---|
| author | Valiollah Hajhashemi Behzad Zolfaghari Pooya Amin |
| author_facet | Valiollah Hajhashemi Behzad Zolfaghari Pooya Amin |
| author_sort | Valiollah Hajhashemi |
| collection | DOAJ |
| description | Objective: Several species of Pinus have shown anti-nociceptive and anti-inflammatory properties. This study was aimed to evaluate anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract of bark and essential oil of leaves of P. eldarica in animal models. Materials and Methods: Hydroalcoholic extract of bark and essential oil of leaves of P. eldarica were prepared and phenolic content and essential oil composition were analyzed using Folin-Ciocalteu method and GC/MS, respectively. Anti-nociceptive effect was assessed using acetic acid, formalin and hot plate tests in male Swiss mice (25-30 g) and for evaluation of anti-inflammatory activity, carrageenan test in male Wistar rats (180-200 g) and croton oil-induced ear edema in male mice, were used. Involvement of opioid, α2-adrenergic, 5-HT3 receptors and adenosine triphosphate (ATP)-dependent K+ channels in pain relief was tested using naloxone, ondansetron, yohimbine and glibenclamide. Results: The total phenolic content of the extract in terms of gallic acid equivalent was 404.9±7.7 mg/g of the extract powder. P. eldarica hydroalcoholic extract (200 and 400 mg/kg) and essential oil (100 and 200 µl/kg) significantly (all, p <0.001) decreased pain behavior in acetic acid and formalin tests but not in hot plate test. The extract and essential oil suppressed edema in carrageenan and croton tests. Glibenclamide partially reversed the anti-nociceptive effect of hydroalcoholic extract while the other antagonists were ineffective. Conclusion: Hydroalcoholic extract of bark and essential oil of leaves of P. eldarica significantly decreased acute and chronic pain as well as inflammation. ATP-dependent K+ channels mediate a part of the observed anti-nociceptive effect. |
| first_indexed | 2024-12-17T10:08:31Z |
| format | Article |
| id | doaj.art-c399d4ceab5f42c28be07ee1eaf8cc16 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-17T10:08:31Z |
| publishDate | 2021-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-c399d4ceab5f42c28be07ee1eaf8cc162022-12-21T21:53:06ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492021-09-0111549450410.22038/ajp.2021.1803818038Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal modelsValiollah Hajhashemi0Behzad Zolfaghari1Pooya Amin2Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranObjective: Several species of Pinus have shown anti-nociceptive and anti-inflammatory properties. This study was aimed to evaluate anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract of bark and essential oil of leaves of P. eldarica in animal models. Materials and Methods: Hydroalcoholic extract of bark and essential oil of leaves of P. eldarica were prepared and phenolic content and essential oil composition were analyzed using Folin-Ciocalteu method and GC/MS, respectively. Anti-nociceptive effect was assessed using acetic acid, formalin and hot plate tests in male Swiss mice (25-30 g) and for evaluation of anti-inflammatory activity, carrageenan test in male Wistar rats (180-200 g) and croton oil-induced ear edema in male mice, were used. Involvement of opioid, α2-adrenergic, 5-HT3 receptors and adenosine triphosphate (ATP)-dependent K+ channels in pain relief was tested using naloxone, ondansetron, yohimbine and glibenclamide. Results: The total phenolic content of the extract in terms of gallic acid equivalent was 404.9±7.7 mg/g of the extract powder. P. eldarica hydroalcoholic extract (200 and 400 mg/kg) and essential oil (100 and 200 µl/kg) significantly (all, p <0.001) decreased pain behavior in acetic acid and formalin tests but not in hot plate test. The extract and essential oil suppressed edema in carrageenan and croton tests. Glibenclamide partially reversed the anti-nociceptive effect of hydroalcoholic extract while the other antagonists were ineffective. Conclusion: Hydroalcoholic extract of bark and essential oil of leaves of P. eldarica significantly decreased acute and chronic pain as well as inflammation. ATP-dependent K+ channels mediate a part of the observed anti-nociceptive effect.https://ajp.mums.ac.ir/article_18038_d6f5e6462a6a90df615c8c4072d75fec.pdfanalgesicanti-inflammationherbal medicinepinus eldarica |
| spellingShingle | Valiollah Hajhashemi Behzad Zolfaghari Pooya Amin Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models Avicenna Journal of Phytomedicine analgesic anti-inflammation herbal medicine pinus eldarica |
| title | Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models |
| title_full | Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models |
| title_fullStr | Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models |
| title_full_unstemmed | Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models |
| title_short | Anti-nociceptive and anti-inflammatory effects of hydroalcoholic extract and essential oil of Pinus eldarica in animal models |
| title_sort | anti nociceptive and anti inflammatory effects of hydroalcoholic extract and essential oil of pinus eldarica in animal models |
| topic | analgesic anti-inflammation herbal medicine pinus eldarica |
| url | https://ajp.mums.ac.ir/article_18038_d6f5e6462a6a90df615c8c4072d75fec.pdf |
| work_keys_str_mv | AT valiollahhajhashemi antinociceptiveandantiinflammatoryeffectsofhydroalcoholicextractandessentialoilofpinuseldaricainanimalmodels AT behzadzolfaghari antinociceptiveandantiinflammatoryeffectsofhydroalcoholicextractandessentialoilofpinuseldaricainanimalmodels AT pooyaamin antinociceptiveandantiinflammatoryeffectsofhydroalcoholicextractandessentialoilofpinuseldaricainanimalmodels |