Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies
<p>Abstract</p> <p>Background</p> <p>The development of non-small cell lung carcinoma proceeds through a series of well-defined pathological steps before the appearance of invasive lung carcinoma. The molecular changes that correspond with pathology changes are not well...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-12-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/5/155 |
| _version_ | 1811333289562079232 |
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| author | Lam Stephen Biddinger Paul W leRiche Jean C Zhang Yu Tichelaar Jay W Anderson Marshall W |
| author_facet | Lam Stephen Biddinger Paul W leRiche Jean C Zhang Yu Tichelaar Jay W Anderson Marshall W |
| author_sort | Lam Stephen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The development of non-small cell lung carcinoma proceeds through a series of well-defined pathological steps before the appearance of invasive lung carcinoma. The molecular changes that correspond with pathology changes are not well defined and identification of the molecular events may provide clues on the progression of intraepithelial neoplasia in the lung, as well as suggest potential targets for chemoprevention. The acquisition of anti-apoptotic signals is critical for the survival of cancer cells but the pathways involved are incompletely characterized in developing intra-epithelial neoplasia (IEN).</p> <p>Methods</p> <p>We used immunohistochemistry to determine the presence, relative levels, and localization of proteins that mediate anti-apoptotic pathways in developing human bronchial neoplasia.</p> <p>Results</p> <p>Bronchial epithelial protein levels of the phosphorylated (active) form of AKT kinase and the caspase inhibitor cIAP-2 were increased in more advanced grades of bronchial IEN lesions than in normal bronchial epithelium. Additionally, the percentage of biopsies with nuclear localization of p65/RELA in epithelial cells increased with advancing pathology grade, suggesting that NF-κB transcriptional activity was induced more frequently in advanced IEN lesions.</p> <p>Conclusion</p> <p>Our results indicate that anti-apoptotic pathways are elevated in bronchial IEN lesions prior to the onset of invasive carcinoma and that targeting these pathways therapeutically may offer promise in prevention of non-small cell lung carcinoma.</p> |
| first_indexed | 2024-04-13T16:50:24Z |
| format | Article |
| id | doaj.art-c39a729b4d4d48e896ecbbe802f85002 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T16:50:24Z |
| publishDate | 2005-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-c39a729b4d4d48e896ecbbe802f850022022-12-22T02:38:58ZengBMCBMC Cancer1471-24072005-12-015115510.1186/1471-2407-5-155Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsiesLam StephenBiddinger Paul WleRiche Jean CZhang YuTichelaar Jay WAnderson Marshall W<p>Abstract</p> <p>Background</p> <p>The development of non-small cell lung carcinoma proceeds through a series of well-defined pathological steps before the appearance of invasive lung carcinoma. The molecular changes that correspond with pathology changes are not well defined and identification of the molecular events may provide clues on the progression of intraepithelial neoplasia in the lung, as well as suggest potential targets for chemoprevention. The acquisition of anti-apoptotic signals is critical for the survival of cancer cells but the pathways involved are incompletely characterized in developing intra-epithelial neoplasia (IEN).</p> <p>Methods</p> <p>We used immunohistochemistry to determine the presence, relative levels, and localization of proteins that mediate anti-apoptotic pathways in developing human bronchial neoplasia.</p> <p>Results</p> <p>Bronchial epithelial protein levels of the phosphorylated (active) form of AKT kinase and the caspase inhibitor cIAP-2 were increased in more advanced grades of bronchial IEN lesions than in normal bronchial epithelium. Additionally, the percentage of biopsies with nuclear localization of p65/RELA in epithelial cells increased with advancing pathology grade, suggesting that NF-κB transcriptional activity was induced more frequently in advanced IEN lesions.</p> <p>Conclusion</p> <p>Our results indicate that anti-apoptotic pathways are elevated in bronchial IEN lesions prior to the onset of invasive carcinoma and that targeting these pathways therapeutically may offer promise in prevention of non-small cell lung carcinoma.</p>http://www.biomedcentral.com/1471-2407/5/155 |
| spellingShingle | Lam Stephen Biddinger Paul W leRiche Jean C Zhang Yu Tichelaar Jay W Anderson Marshall W Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies BMC Cancer |
| title | Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies |
| title_full | Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies |
| title_fullStr | Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies |
| title_full_unstemmed | Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies |
| title_short | Increased staining for phospho-Akt, p65/RELA and cIAP-2 in pre-neoplastic human bronchial biopsies |
| title_sort | increased staining for phospho akt p65 rela and ciap 2 in pre neoplastic human bronchial biopsies |
| url | http://www.biomedcentral.com/1471-2407/5/155 |
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