Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications
The effect of the light of different spectral compositions, white fluorescent light (WFL), red light (RL, 660 nm), blue light (BL, 450 nm), green light (GL, 525 nm), and white LED light (WL, 450 + 580 nm), on the physiological parameters of <i>Solanum lycopersicum</i> 3005 <i>hp-2&...
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| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/10149 |
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| author | Mikhail Vereshchagin Vladimir Kreslavski Yury Ivanov Alexandra Ivanova Tamara Kumachova Andrey Ryabchenko Anatoliy Kosobryukhov Vladimir Kuznetsov Pavel Pashkovskiy |
| author_facet | Mikhail Vereshchagin Vladimir Kreslavski Yury Ivanov Alexandra Ivanova Tamara Kumachova Andrey Ryabchenko Anatoliy Kosobryukhov Vladimir Kuznetsov Pavel Pashkovskiy |
| author_sort | Mikhail Vereshchagin |
| collection | DOAJ |
| description | The effect of the light of different spectral compositions, white fluorescent light (WFL), red light (RL, 660 nm), blue light (BL, 450 nm), green light (GL, 525 nm), and white LED light (WL, 450 + 580 nm), on the physiological parameters of <i>Solanum lycopersicum</i> 3005 <i>hp-2</i> (defective for a <i>DET1</i> gene) and 4012 <i>hp-1w</i>; 3538 <i>hp-1</i>; 0279 <i>hp-1.2</i> (defective for a <i>DDB1a</i> gene) photomorphogenetic mutants was studied. The parameters of the primary photochemical processes of photosynthesis, photosynthetic and transpiration rates, the antioxidant capacity of low-molecular weight antioxidants, the content of the total phenolic compounds, including flavonoids, and the expression of the genes involved in light signaling and biosynthesis of secondary metabolites were determined. Under BL, the 3005 <i>hp-2</i> mutant showed the highest nonenzymatic antioxidant activity, which occurred to a greater extent due to the increase in flavonoid content. At the same time, under BL, the number of secretory trichomes on the surface of the leaves of all mutants increased equally. This suggests the accumulation of flavonoids inside leaf cells rather than in trichomes on the leaf surface. The data obtained indicate the possibility of using the <i>hp-2</i> mutant for biotechnology to increase its nutritional value by enhancing the content of flavonoids and other antioxidants by modulating the spectral composition of light. |
| first_indexed | 2024-03-11T02:21:49Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:21:49Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c39c9b2512fe4f65953c7bb9e1cc6e7e2023-11-18T10:49:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121014910.3390/ijms241210149Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological ApplicationsMikhail Vereshchagin0Vladimir Kreslavski1Yury Ivanov2Alexandra Ivanova3Tamara Kumachova4Andrey Ryabchenko5Anatoliy Kosobryukhov6Vladimir Kuznetsov7Pavel Pashkovskiy8K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaInstitute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino 142290, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaDepartment of Plant Physiology, Moscow Timiryazev Agricultural Academy, Russian State Agrarian University, Timiryazevskaya Street 49, Moscow 127550, RussiaTsitsin Main Botanical Garden, Russian Academy of Sciences, Botanicheskaya Street 4, Moscow 127276, RussiaInstitute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino 142290, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow 127276, RussiaThe effect of the light of different spectral compositions, white fluorescent light (WFL), red light (RL, 660 nm), blue light (BL, 450 nm), green light (GL, 525 nm), and white LED light (WL, 450 + 580 nm), on the physiological parameters of <i>Solanum lycopersicum</i> 3005 <i>hp-2</i> (defective for a <i>DET1</i> gene) and 4012 <i>hp-1w</i>; 3538 <i>hp-1</i>; 0279 <i>hp-1.2</i> (defective for a <i>DDB1a</i> gene) photomorphogenetic mutants was studied. The parameters of the primary photochemical processes of photosynthesis, photosynthetic and transpiration rates, the antioxidant capacity of low-molecular weight antioxidants, the content of the total phenolic compounds, including flavonoids, and the expression of the genes involved in light signaling and biosynthesis of secondary metabolites were determined. Under BL, the 3005 <i>hp-2</i> mutant showed the highest nonenzymatic antioxidant activity, which occurred to a greater extent due to the increase in flavonoid content. At the same time, under BL, the number of secretory trichomes on the surface of the leaves of all mutants increased equally. This suggests the accumulation of flavonoids inside leaf cells rather than in trichomes on the leaf surface. The data obtained indicate the possibility of using the <i>hp-2</i> mutant for biotechnology to increase its nutritional value by enhancing the content of flavonoids and other antioxidants by modulating the spectral composition of light.https://www.mdpi.com/1422-0067/24/12/10149red lightblue lightgreen lightphotomorphogenetic mutantsflavonoid content |
| spellingShingle | Mikhail Vereshchagin Vladimir Kreslavski Yury Ivanov Alexandra Ivanova Tamara Kumachova Andrey Ryabchenko Anatoliy Kosobryukhov Vladimir Kuznetsov Pavel Pashkovskiy Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications International Journal of Molecular Sciences red light blue light green light photomorphogenetic mutants flavonoid content |
| title | Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications |
| title_full | Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications |
| title_fullStr | Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications |
| title_full_unstemmed | Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications |
| title_short | Investigating the Physiological and Molecular Responses of <i>Solanum lycopersicum hp</i> Mutants to Light of Different Quality for Biotechnological Applications |
| title_sort | investigating the physiological and molecular responses of i solanum lycopersicum hp i mutants to light of different quality for biotechnological applications |
| topic | red light blue light green light photomorphogenetic mutants flavonoid content |
| url | https://www.mdpi.com/1422-0067/24/12/10149 |
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