Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch
Recent studies have shown that nanomaterials, including carbon nanotubes, are associated with a wide range of effects on living organisms, from stimulation to toxic effects. Plants are an important object of such research, which is associated with the potential use of carbon nanomaterials in agricul...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/1/163 |
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| author | Konstantin V. Zhuzhukin Peter M. Evlakov Tatiana A. Grodetskaya Alexander A. Gusev Olga V. Zakharova Aleksey V. Shuklinov Elena V. Tomina |
| author_facet | Konstantin V. Zhuzhukin Peter M. Evlakov Tatiana A. Grodetskaya Alexander A. Gusev Olga V. Zakharova Aleksey V. Shuklinov Elena V. Tomina |
| author_sort | Konstantin V. Zhuzhukin |
| collection | DOAJ |
| description | Recent studies have shown that nanomaterials, including carbon nanotubes, are associated with a wide range of effects on living organisms, from stimulation to toxic effects. Plants are an important object of such research, which is associated with the potential use of carbon nanomaterials in agriculture and environmental protection. At the same time, the specific mechanisms of formation of plant resistance to the effects of carbon nanotubes remain not fully understood, especially in woody plants. Therefore, we studied the effect of aqueous colloids of multi-walled carbon nanotubes (MWCNTs) with an outer diameter of 10–30 nm and a length of about 2 μm at a concentration of 1, 10, 50, and 100 mg/L on morphometric parameters and the level of expression of stress resistance genes in <i>Betula pubescens</i> Ehrh. and <i>B. pendula</i> Roth. plants in greenhouse conditions. The results showed an increase in the length and diameter of the shoot in the studied plants. The dry biomass of the leaf increased by 30%, the stem by 42%, and the root by 49% when using MWCNTs at a concentration of 10 mg/L. The expression of the stress resistance genes <i>DREB2</i> and <i>PR-10</i> significantly increased under the influence of 1 mg/L MWCNTs on plants of both species. At the same time, the use of 100 mg/L nanoparticles led to a decrease in the studied parameters in <i>Betula pendula</i>, which may be associated with the negative effect of MWCNTs in high concentrations. The revealed positive effects of low concentrations of MWCNTs on morphometric parameters and stimulation of stress resistance genes by nanotubes open up prospects for their use in woody plant biotechnology. |
| first_indexed | 2024-03-09T12:41:12Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T12:41:12Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-c39e5559cbe6467cb8517e70b76f88f62023-11-30T22:18:37ZengMDPI AGForests1999-49072023-01-0114116310.3390/f14010163Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in BirchKonstantin V. Zhuzhukin0Peter M. Evlakov1Tatiana A. Grodetskaya2Alexander A. Gusev3Olga V. Zakharova4Aleksey V. Shuklinov5Elena V. Tomina6Department of Chemistry, Voronezh State University of Forestry and Technologies Named after G.F. Morozov, 8, Timiryazeva, 394087 Voronezh, RussiaLaboratory of PCR Analysis, Research Institute of Innovative Technologies of the Forestry Complex, Voronezh State University of Forestry and Technologies Named after G. F. Morozov, 394087 Voronezh, RussiaLaboratory of PCR Analysis, Research Institute of Innovative Technologies of the Forestry Complex, Voronezh State University of Forestry and Technologies Named after G. F. Morozov, 394087 Voronezh, RussiaLaboratory of PCR Analysis, Research Institute of Innovative Technologies of the Forestry Complex, Voronezh State University of Forestry and Technologies Named after G. F. Morozov, 394087 Voronezh, RussiaInstitute for Environmental Science and Biotechnology, Derzhavin Tambov State University, 392020 Tambov, RussiaInstitute for Environmental Science and Biotechnology, Derzhavin Tambov State University, 392020 Tambov, RussiaDepartment of Chemistry, Voronezh State University of Forestry and Technologies Named after G.F. Morozov, 8, Timiryazeva, 394087 Voronezh, RussiaRecent studies have shown that nanomaterials, including carbon nanotubes, are associated with a wide range of effects on living organisms, from stimulation to toxic effects. Plants are an important object of such research, which is associated with the potential use of carbon nanomaterials in agriculture and environmental protection. At the same time, the specific mechanisms of formation of plant resistance to the effects of carbon nanotubes remain not fully understood, especially in woody plants. Therefore, we studied the effect of aqueous colloids of multi-walled carbon nanotubes (MWCNTs) with an outer diameter of 10–30 nm and a length of about 2 μm at a concentration of 1, 10, 50, and 100 mg/L on morphometric parameters and the level of expression of stress resistance genes in <i>Betula pubescens</i> Ehrh. and <i>B. pendula</i> Roth. plants in greenhouse conditions. The results showed an increase in the length and diameter of the shoot in the studied plants. The dry biomass of the leaf increased by 30%, the stem by 42%, and the root by 49% when using MWCNTs at a concentration of 10 mg/L. The expression of the stress resistance genes <i>DREB2</i> and <i>PR-10</i> significantly increased under the influence of 1 mg/L MWCNTs on plants of both species. At the same time, the use of 100 mg/L nanoparticles led to a decrease in the studied parameters in <i>Betula pendula</i>, which may be associated with the negative effect of MWCNTs in high concentrations. The revealed positive effects of low concentrations of MWCNTs on morphometric parameters and stimulation of stress resistance genes by nanotubes open up prospects for their use in woody plant biotechnology.https://www.mdpi.com/1999-4907/14/1/163carbon nanomaterialschanges in growth ratesgene expressionbirch plantsgrowth stimulation |
| spellingShingle | Konstantin V. Zhuzhukin Peter M. Evlakov Tatiana A. Grodetskaya Alexander A. Gusev Olga V. Zakharova Aleksey V. Shuklinov Elena V. Tomina Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch Forests carbon nanomaterials changes in growth rates gene expression birch plants growth stimulation |
| title | Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch |
| title_full | Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch |
| title_fullStr | Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch |
| title_full_unstemmed | Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch |
| title_short | Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch |
| title_sort | effect of multi walled carbon nanotubes on the growth and expression of stress resistance genes in birch |
| topic | carbon nanomaterials changes in growth rates gene expression birch plants growth stimulation |
| url | https://www.mdpi.com/1999-4907/14/1/163 |
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