Glial cell changes in the corpus callosum in chronically-starved mice
Abstract Anorexia nervosa (AN) is characterized by emaciation, hyperactivity, and amenorrhea. Imaging studies in AN patients have revealed reductions in grey and white matter volume, which correlate with the severity of neuropsychological deficits. However, the cellular basis for the observed brain...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Eating Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40337-023-00948-z |
| _version_ | 1797377223186972672 |
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| author | Annelie Zimmermann Natalie Böge Katharina Schuster Anna Staffeld Stephan Lang Sadaf Gill Hanna Rupprecht Linda Frintrop |
| author_facet | Annelie Zimmermann Natalie Böge Katharina Schuster Anna Staffeld Stephan Lang Sadaf Gill Hanna Rupprecht Linda Frintrop |
| author_sort | Annelie Zimmermann |
| collection | DOAJ |
| description | Abstract Anorexia nervosa (AN) is characterized by emaciation, hyperactivity, and amenorrhea. Imaging studies in AN patients have revealed reductions in grey and white matter volume, which correlate with the severity of neuropsychological deficits. However, the cellular basis for the observed brain atrophy is poorly understood. Although distinct hypothalamic centers, including the arcuate nucleus (ARC) are critically involved in regulating feeding behavior, little is known about potential hypothalamic modifications in this disorder. Since glia e.g. astrocytes and microglia influence neuronal circuits, we investigated the glial changes underlying pathophysiology of starvation in the corpus callosum (CC) and hypothalamus. Female mice were given a limited amount of food once a day and had unlimited access to a running wheel until a 20% weight reduction was achieved (acute starvation). This weight reduction was maintained for two weeks to mimic chronic starvation. Immunohistochemistry was used to quantify the density of astrocytes, microglia, oligodendrocytes, and the staining intensity of neuropeptide Y (NPY), a potent orexigenic peptide. Chronic starvation induced a decreased density of OLIG2+ oligodendrocytes, GFAP+ astrocytes, and IBA1+ microglia in the CC. However, the densities of glial cells remained unchanged in the ARC following starvation. Additionally, the staining intensity of NPY increased after both acute and chronic starvation, indicating an increased orexigenic signaling. Chronic starvation induced glial cell changes in the CC in a mouse model of AN suggesting that glia pathophysiology may play a role in the disease. |
| first_indexed | 2024-03-08T19:50:39Z |
| format | Article |
| id | doaj.art-c3a457ab10b049f39526d1cbf13d3524 |
| institution | Directory Open Access Journal |
| issn | 2050-2974 |
| language | English |
| last_indexed | 2024-03-08T19:50:39Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Eating Disorders |
| spelling | doaj.art-c3a457ab10b049f39526d1cbf13d35242023-12-24T12:07:35ZengBMCJournal of Eating Disorders2050-29742023-12-011111910.1186/s40337-023-00948-zGlial cell changes in the corpus callosum in chronically-starved miceAnnelie Zimmermann0Natalie Böge1Katharina Schuster2Anna Staffeld3Stephan Lang4Sadaf Gill5Hanna Rupprecht6Linda Frintrop7Institute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterInstitute of Anatomy, Rostock University Medical CenterAbstract Anorexia nervosa (AN) is characterized by emaciation, hyperactivity, and amenorrhea. Imaging studies in AN patients have revealed reductions in grey and white matter volume, which correlate with the severity of neuropsychological deficits. However, the cellular basis for the observed brain atrophy is poorly understood. Although distinct hypothalamic centers, including the arcuate nucleus (ARC) are critically involved in regulating feeding behavior, little is known about potential hypothalamic modifications in this disorder. Since glia e.g. astrocytes and microglia influence neuronal circuits, we investigated the glial changes underlying pathophysiology of starvation in the corpus callosum (CC) and hypothalamus. Female mice were given a limited amount of food once a day and had unlimited access to a running wheel until a 20% weight reduction was achieved (acute starvation). This weight reduction was maintained for two weeks to mimic chronic starvation. Immunohistochemistry was used to quantify the density of astrocytes, microglia, oligodendrocytes, and the staining intensity of neuropeptide Y (NPY), a potent orexigenic peptide. Chronic starvation induced a decreased density of OLIG2+ oligodendrocytes, GFAP+ astrocytes, and IBA1+ microglia in the CC. However, the densities of glial cells remained unchanged in the ARC following starvation. Additionally, the staining intensity of NPY increased after both acute and chronic starvation, indicating an increased orexigenic signaling. Chronic starvation induced glial cell changes in the CC in a mouse model of AN suggesting that glia pathophysiology may play a role in the disease.https://doi.org/10.1186/s40337-023-00948-zAnorexia nervosaSemi-starvation induced hyperactivityAstrocytesMicroglia cellsOligodendrocytes |
| spellingShingle | Annelie Zimmermann Natalie Böge Katharina Schuster Anna Staffeld Stephan Lang Sadaf Gill Hanna Rupprecht Linda Frintrop Glial cell changes in the corpus callosum in chronically-starved mice Journal of Eating Disorders Anorexia nervosa Semi-starvation induced hyperactivity Astrocytes Microglia cells Oligodendrocytes |
| title | Glial cell changes in the corpus callosum in chronically-starved mice |
| title_full | Glial cell changes in the corpus callosum in chronically-starved mice |
| title_fullStr | Glial cell changes in the corpus callosum in chronically-starved mice |
| title_full_unstemmed | Glial cell changes in the corpus callosum in chronically-starved mice |
| title_short | Glial cell changes in the corpus callosum in chronically-starved mice |
| title_sort | glial cell changes in the corpus callosum in chronically starved mice |
| topic | Anorexia nervosa Semi-starvation induced hyperactivity Astrocytes Microglia cells Oligodendrocytes |
| url | https://doi.org/10.1186/s40337-023-00948-z |
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