Coordinated genomic control of ciliogenesis and cell movement by RFX2
The mechanisms linking systems-level programs of gene expression to discrete cell biological processes in vivo remain poorly understood. In this study, we have defined such a program for multi-ciliated epithelial cells (MCCs), a cell type critical for proper development and homeostasis of the airway...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01439 |
| _version_ | 1811180437507145728 |
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| author | Mei-I Chung Taejoon Kwon Fan Tu Eric R Brooks Rakhi Gupta Matthew Meyer Julie C Baker Edward M Marcotte John B Wallingford |
| author_facet | Mei-I Chung Taejoon Kwon Fan Tu Eric R Brooks Rakhi Gupta Matthew Meyer Julie C Baker Edward M Marcotte John B Wallingford |
| author_sort | Mei-I Chung |
| collection | DOAJ |
| description | The mechanisms linking systems-level programs of gene expression to discrete cell biological processes in vivo remain poorly understood. In this study, we have defined such a program for multi-ciliated epithelial cells (MCCs), a cell type critical for proper development and homeostasis of the airway, brain and reproductive tracts. Starting from genomic analysis of the cilia-associated transcription factor Rfx2, we used bioinformatics and in vivo cell biological approaches to gain insights into the molecular basis of cilia assembly and function. Moreover, we discovered a previously un-recognized role for an Rfx factor in cell movement, finding that Rfx2 cell-autonomously controls apical surface expansion in nascent MCCs. Thus, Rfx2 coordinates multiple, distinct gene expression programs in MCCs, regulating genes that control cell movement, ciliogenesis, and cilia function. As such, the work serves as a paradigm for understanding genomic control of cell biological processes that span from early cell morphogenetic events to terminally differentiated cellular functions. |
| first_indexed | 2024-04-11T09:03:08Z |
| format | Article |
| id | doaj.art-c3a6cc19af7c42d98759270e42cfea86 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:03:08Z |
| publishDate | 2014-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c3a6cc19af7c42d98759270e42cfea862022-12-22T04:32:43ZengeLife Sciences Publications LtdeLife2050-084X2014-01-01310.7554/eLife.01439Coordinated genomic control of ciliogenesis and cell movement by RFX2Mei-I Chung0Taejoon Kwon1Fan Tu2Eric R Brooks3Rakhi Gupta4Matthew Meyer5Julie C Baker6Edward M Marcotte7John B Wallingford8Department of Molecular Biosciences, University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United StatesDepartment of Genetics, Stanford University, Stanford, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United StatesDepartment of Genetics, Stanford University, Stanford, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United States; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, University of Texas at Austin, Austin, United States; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, United States; Howard Hughes Medical Institute, University of Texas at Austin, Austin, United StatesThe mechanisms linking systems-level programs of gene expression to discrete cell biological processes in vivo remain poorly understood. In this study, we have defined such a program for multi-ciliated epithelial cells (MCCs), a cell type critical for proper development and homeostasis of the airway, brain and reproductive tracts. Starting from genomic analysis of the cilia-associated transcription factor Rfx2, we used bioinformatics and in vivo cell biological approaches to gain insights into the molecular basis of cilia assembly and function. Moreover, we discovered a previously un-recognized role for an Rfx factor in cell movement, finding that Rfx2 cell-autonomously controls apical surface expansion in nascent MCCs. Thus, Rfx2 coordinates multiple, distinct gene expression programs in MCCs, regulating genes that control cell movement, ciliogenesis, and cilia function. As such, the work serves as a paradigm for understanding genomic control of cell biological processes that span from early cell morphogenetic events to terminally differentiated cellular functions.https://elifesciences.org/articles/01439ciliamulticiliated cellmucociliary epitheliumcilia beatingRfx2genomic |
| spellingShingle | Mei-I Chung Taejoon Kwon Fan Tu Eric R Brooks Rakhi Gupta Matthew Meyer Julie C Baker Edward M Marcotte John B Wallingford Coordinated genomic control of ciliogenesis and cell movement by RFX2 eLife cilia multiciliated cell mucociliary epithelium cilia beating Rfx2 genomic |
| title | Coordinated genomic control of ciliogenesis and cell movement by RFX2 |
| title_full | Coordinated genomic control of ciliogenesis and cell movement by RFX2 |
| title_fullStr | Coordinated genomic control of ciliogenesis and cell movement by RFX2 |
| title_full_unstemmed | Coordinated genomic control of ciliogenesis and cell movement by RFX2 |
| title_short | Coordinated genomic control of ciliogenesis and cell movement by RFX2 |
| title_sort | coordinated genomic control of ciliogenesis and cell movement by rfx2 |
| topic | cilia multiciliated cell mucociliary epithelium cilia beating Rfx2 genomic |
| url | https://elifesciences.org/articles/01439 |
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