A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR
<p>Abstract</p> <p>Background</p> <p>Surveillance for drug-resistant <it>Plasmodium falciparum </it>should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene ampli...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-05-01
|
| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/3/1/9 |
| _version_ | 1818351553454538752 |
|---|---|
| author | Welch Kathy Miller R Scott McDaniel Phillip Congpuong Kanungnij Laoboonchai Anita Nelson Amy Purfield Anne Wongsrichanalai Chansuda Meshnick Steven R |
| author_facet | Welch Kathy Miller R Scott McDaniel Phillip Congpuong Kanungnij Laoboonchai Anita Nelson Amy Purfield Anne Wongsrichanalai Chansuda Meshnick Steven R |
| author_sort | Welch Kathy |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Surveillance for drug-resistant <it>Plasmodium falciparum </it>should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene amplification could be useful survellance tools.</p> <p>Methods</p> <p>A real-time PCR assay has been developed that identifies single nucleotide polymorphisms (SNPs) at amino acids 86, 184, 1034 and 1042 in the <it>P. falciparum </it>multi-drug resistant (<it>pfmdr 1</it>) gene that may be associated with anti-malarial drug resistance.</p> <p>Results</p> <p>This assay has a sensitivity and specificity of 94% and 100% when compared to traditional PCR methods for genotyping. Only 54 of 68 (79%) paired pre- and post-culture DNA samples were concordant at all four loci.</p> <p>Conclusion</p> <p>Real-time PCR is a sensitive and specific method to detect SNP's in <it>pfmdr 1</it>. Genotypes of parasites after <it>in vitro </it>culture may not reflect that seen <it>in vivo</it>.</p> |
| first_indexed | 2024-12-13T18:39:34Z |
| format | Article |
| id | doaj.art-c3b4058a04534c7fb34599f5b25da1fd |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-13T18:39:34Z |
| publishDate | 2004-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-c3b4058a04534c7fb34599f5b25da1fd2022-12-21T23:35:16ZengBMCMalaria Journal1475-28752004-05-0131910.1186/1475-2875-3-9A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCRWelch KathyMiller R ScottMcDaniel PhillipCongpuong KanungnijLaoboonchai AnitaNelson AmyPurfield AnneWongsrichanalai ChansudaMeshnick Steven R<p>Abstract</p> <p>Background</p> <p>Surveillance for drug-resistant <it>Plasmodium falciparum </it>should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene amplification could be useful survellance tools.</p> <p>Methods</p> <p>A real-time PCR assay has been developed that identifies single nucleotide polymorphisms (SNPs) at amino acids 86, 184, 1034 and 1042 in the <it>P. falciparum </it>multi-drug resistant (<it>pfmdr 1</it>) gene that may be associated with anti-malarial drug resistance.</p> <p>Results</p> <p>This assay has a sensitivity and specificity of 94% and 100% when compared to traditional PCR methods for genotyping. Only 54 of 68 (79%) paired pre- and post-culture DNA samples were concordant at all four loci.</p> <p>Conclusion</p> <p>Real-time PCR is a sensitive and specific method to detect SNP's in <it>pfmdr 1</it>. Genotypes of parasites after <it>in vitro </it>culture may not reflect that seen <it>in vivo</it>.</p>http://www.malariajournal.com/content/3/1/9 |
| spellingShingle | Welch Kathy Miller R Scott McDaniel Phillip Congpuong Kanungnij Laoboonchai Anita Nelson Amy Purfield Anne Wongsrichanalai Chansuda Meshnick Steven R A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR Malaria Journal |
| title | A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR |
| title_full | A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR |
| title_fullStr | A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR |
| title_full_unstemmed | A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR |
| title_short | A new method for detection of <it>pfmdr1 </it>mutations in <it>Plasmodium falciparum </it>DNA using real-time PCR |
| title_sort | new method for detection of it pfmdr1 it mutations in it plasmodium falciparum it dna using real time pcr |
| url | http://www.malariajournal.com/content/3/1/9 |
| work_keys_str_mv | AT welchkathy anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT millerrscott anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT mcdanielphillip anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT congpuongkanungnij anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT laoboonchaianita anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT nelsonamy anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT purfieldanne anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT wongsrichanalaichansuda anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT meshnickstevenr anewmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT welchkathy newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT millerrscott newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT mcdanielphillip newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT congpuongkanungnij newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT laoboonchaianita newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT nelsonamy newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT purfieldanne newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT wongsrichanalaichansuda newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr AT meshnickstevenr newmethodfordetectionofitpfmdr1itmutationsinitplasmodiumfalciparumitdnausingrealtimepcr |