Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats
In vivo studies of the therapeutic effects of argon in traumatic brain injury (TBI) are limited, and their results are contradictory. The aim of this study was to evaluate the effect of a three-hour inhalation of argon (70%Ar/30%O<sub>2</sub>) after an open TBI on the severity of the neu...
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MDPI AG
2022-07-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/12/7/920 |
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| author | Viktoriya V. Antonova Denis N. Silachev Ivan A. Ryzhkov Konstantin N. Lapin Sergey N. Kalabushev Irina V. Ostrova Lydia A. Varnakova Oleg A. Grebenchikov |
| author_facet | Viktoriya V. Antonova Denis N. Silachev Ivan A. Ryzhkov Konstantin N. Lapin Sergey N. Kalabushev Irina V. Ostrova Lydia A. Varnakova Oleg A. Grebenchikov |
| author_sort | Viktoriya V. Antonova |
| collection | DOAJ |
| description | In vivo studies of the therapeutic effects of argon in traumatic brain injury (TBI) are limited, and their results are contradictory. The aim of this study was to evaluate the effect of a three-hour inhalation of argon (70%Ar/30%O<sub>2</sub>) after an open TBI on the severity of the neurological deficit and the degree of brain damage in rats. The experiments were performed on male Wistar rats (<i>n</i> = 35). The TBI was simulated by the dosed open brain contusion injury. The animals were divided into three groups: sham-operated (SO, <i>n</i> = 7); TBI + 70%N2/30%O<sub>2</sub> (TBI, <i>n</i> = 14); TBI + 70%Ar/30%O<sub>2</sub> (TBI + iAr, <i>n</i> = 14). The Neurological status was assessed over a 14-day period (using the limb-placing and cylinder tests). Magnetic resonance imaging (MRI) scans and a histological examination of the brain with an assessment of the volume of the lesions were performed 14 days after the injury. At each of the time points (days 1, 7, and 14), the limb-placing test score was lower in the TBI and TBI + iAr groups than in the SO group, while there were no significant differences between the TBI and TBI + iAr groups. Additionally, no differences were found between these groups in the cylinder test scores (day 13). The volume of brain damage (tissue loss) according to both the MRI and histological findings did not differ between the TBI and TBI + iAr groups. A three-hour inhalation of argon (70%Ar/30%O<sub>2</sub>) after a TBI had no neuroprotective effect. |
| first_indexed | 2024-03-09T12:09:57Z |
| format | Article |
| id | doaj.art-c3b4eb0673b94278a42d8c2122e54f49 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-09T12:09:57Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-c3b4eb0673b94278a42d8c2122e54f492023-11-30T22:54:08ZengMDPI AGBrain Sciences2076-34252022-07-0112792010.3390/brainsci12070920Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in RatsViktoriya V. Antonova0Denis N. Silachev1Ivan A. Ryzhkov2Konstantin N. Lapin3Sergey N. Kalabushev4Irina V. Ostrova5Lydia A. Varnakova6Oleg A. Grebenchikov7V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaA.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaV.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaIn vivo studies of the therapeutic effects of argon in traumatic brain injury (TBI) are limited, and their results are contradictory. The aim of this study was to evaluate the effect of a three-hour inhalation of argon (70%Ar/30%O<sub>2</sub>) after an open TBI on the severity of the neurological deficit and the degree of brain damage in rats. The experiments were performed on male Wistar rats (<i>n</i> = 35). The TBI was simulated by the dosed open brain contusion injury. The animals were divided into three groups: sham-operated (SO, <i>n</i> = 7); TBI + 70%N2/30%O<sub>2</sub> (TBI, <i>n</i> = 14); TBI + 70%Ar/30%O<sub>2</sub> (TBI + iAr, <i>n</i> = 14). The Neurological status was assessed over a 14-day period (using the limb-placing and cylinder tests). Magnetic resonance imaging (MRI) scans and a histological examination of the brain with an assessment of the volume of the lesions were performed 14 days after the injury. At each of the time points (days 1, 7, and 14), the limb-placing test score was lower in the TBI and TBI + iAr groups than in the SO group, while there were no significant differences between the TBI and TBI + iAr groups. Additionally, no differences were found between these groups in the cylinder test scores (day 13). The volume of brain damage (tissue loss) according to both the MRI and histological findings did not differ between the TBI and TBI + iAr groups. A three-hour inhalation of argon (70%Ar/30%O<sub>2</sub>) after a TBI had no neuroprotective effect.https://www.mdpi.com/2076-3425/12/7/920argonneuroprotectiontraumatic brain injuryorganoprotection |
| spellingShingle | Viktoriya V. Antonova Denis N. Silachev Ivan A. Ryzhkov Konstantin N. Lapin Sergey N. Kalabushev Irina V. Ostrova Lydia A. Varnakova Oleg A. Grebenchikov Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats Brain Sciences argon neuroprotection traumatic brain injury organoprotection |
| title | Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats |
| title_full | Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats |
| title_fullStr | Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats |
| title_full_unstemmed | Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats |
| title_short | Three-Hour Argon Inhalation Has No Neuroprotective Effect after Open Traumatic Brain Injury in Rats |
| title_sort | three hour argon inhalation has no neuroprotective effect after open traumatic brain injury in rats |
| topic | argon neuroprotection traumatic brain injury organoprotection |
| url | https://www.mdpi.com/2076-3425/12/7/920 |
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