Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton
Members of the acyl-CoA-binding protein (ACBP) gene family play vital roles in diverse processes related to lipid metabolism, growth and development, and environmental response. Plant <i>ACBP</i> genes have been well-studied in a variety of species including Arabidopsis, soybean, rice an...
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MDPI AG
2023-04-01
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| Series: | Genes |
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| author | Yizhen Chen Mingchuan Fu Hao Li Liguo Wang Renzhong Liu Zhanji Liu |
| author_facet | Yizhen Chen Mingchuan Fu Hao Li Liguo Wang Renzhong Liu Zhanji Liu |
| author_sort | Yizhen Chen |
| collection | DOAJ |
| description | Members of the acyl-CoA-binding protein (ACBP) gene family play vital roles in diverse processes related to lipid metabolism, growth and development, and environmental response. Plant <i>ACBP</i> genes have been well-studied in a variety of species including Arabidopsis, soybean, rice and maize. However, the identification and functions of <i>ACBP</i> genes in cotton remain to be elucidated. In this study, a total of 11 <i>GaACBP</i>, 12 <i>GrACBP</i>, 20 <i>GbACBP</i>, and 19 <i>GhACBP</i> genes were identified in the genomes of <i>Gossypium arboreum</i>, <i>Gossypium raimondii</i>, <i>Gossypium babardense,</i> and <i>Gossypium hirsutum</i>, respectively, and grouped into four clades. Forty-nine duplicated gene pairs were identified in <i>Gossypium ACBP</i> genes, and almost all of which have undergone purifying selection during the long evolutionary process. In addition, expression analyses showed that most of the <i>GhACBP</i> genes were highly expressed in the developing embryos. Furthermore, <i>GhACBP1</i> and <i>GhACBP2</i> were induced by salt and drought stress based on a real-time quantitative PCR (RT-qPCR) assay, indicating that these genes may play an important role in salt- and drought-stress tolerance. This study will provide a basic resource for further functional analysis of the <i>ACBP</i> gene family in cotton. |
| first_indexed | 2024-03-11T04:59:22Z |
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| id | doaj.art-c3bd26bd61a0462f856f3e90cb3299df |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T04:59:22Z |
| publishDate | 2023-04-01 |
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| series | Genes |
| spelling | doaj.art-c3bd26bd61a0462f856f3e90cb3299df2023-11-17T19:23:32ZengMDPI AGGenes2073-44252023-04-0114485910.3390/genes14040859Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in CottonYizhen Chen0Mingchuan Fu1Hao Li2Liguo Wang3Renzhong Liu4Zhanji Liu5Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Industrial Crops Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaMembers of the acyl-CoA-binding protein (ACBP) gene family play vital roles in diverse processes related to lipid metabolism, growth and development, and environmental response. Plant <i>ACBP</i> genes have been well-studied in a variety of species including Arabidopsis, soybean, rice and maize. However, the identification and functions of <i>ACBP</i> genes in cotton remain to be elucidated. In this study, a total of 11 <i>GaACBP</i>, 12 <i>GrACBP</i>, 20 <i>GbACBP</i>, and 19 <i>GhACBP</i> genes were identified in the genomes of <i>Gossypium arboreum</i>, <i>Gossypium raimondii</i>, <i>Gossypium babardense,</i> and <i>Gossypium hirsutum</i>, respectively, and grouped into four clades. Forty-nine duplicated gene pairs were identified in <i>Gossypium ACBP</i> genes, and almost all of which have undergone purifying selection during the long evolutionary process. In addition, expression analyses showed that most of the <i>GhACBP</i> genes were highly expressed in the developing embryos. Furthermore, <i>GhACBP1</i> and <i>GhACBP2</i> were induced by salt and drought stress based on a real-time quantitative PCR (RT-qPCR) assay, indicating that these genes may play an important role in salt- and drought-stress tolerance. This study will provide a basic resource for further functional analysis of the <i>ACBP</i> gene family in cotton.https://www.mdpi.com/2073-4425/14/4/859cotton<i>ACBP</i> gene familyexpression analysissalt and drought stress |
| spellingShingle | Yizhen Chen Mingchuan Fu Hao Li Liguo Wang Renzhong Liu Zhanji Liu Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton Genes cotton <i>ACBP</i> gene family expression analysis salt and drought stress |
| title | Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton |
| title_full | Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton |
| title_fullStr | Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton |
| title_full_unstemmed | Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton |
| title_short | Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton |
| title_sort | molecular characterization of the acyl coa binding protein genes reveals their significant roles in oil accumulation and abiotic stress response in cotton |
| topic | cotton <i>ACBP</i> gene family expression analysis salt and drought stress |
| url | https://www.mdpi.com/2073-4425/14/4/859 |
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