Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626
The ascomycetous yeast <i>Candida membranifaciens</i> has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated fo...
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MDPI AG
2022-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/3/254 |
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| author | Margarida Palma Stephen Mondo Mariana Pereira Érica Vieira Igor V. Grigoriev Isabel Sá-Correia |
| author_facet | Margarida Palma Stephen Mondo Mariana Pereira Érica Vieira Igor V. Grigoriev Isabel Sá-Correia |
| author_sort | Margarida Palma |
| collection | DOAJ |
| description | The ascomycetous yeast <i>Candida membranifaciens</i> has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated four <i>C. membranifaciens</i> strains from soil and investigated their potential to overproduce riboflavin. <i>C. membranifaciens</i> IST 626 was found to produce the highest concentrations of riboflavin. The volumetric production of this vitamin was higher when <i>C. membranifaciens</i> IST 626 cells were cultured in a commercial medium without iron and when xylose was the available carbon source compared to the same basal medium with glucose. Supplementation of the growth medium with 2 g/L glycine favored the metabolization of xylose, leading to biomass increase and consequent enhancement of riboflavin volumetric production that reached 120 mg/L after 216 h of cultivation. To gain new insights into the molecular basis of riboflavin production and carbon source utilization in this species, the first annotated genome sequence of <i>C. membranifaciens</i> is reported in this article, as well as the result of a comparative genomic analysis with other relevant yeast species. A total of 5619 genes were predicted to be present in <i>C. membranifaciens</i> IST 626 genome sequence (11.5 Mbp). Among them are genes involved in riboflavin biosynthesis, iron homeostasis, and sugar uptake and metabolism. This work put forward <i>C. membranifaciens</i> IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species. |
| first_indexed | 2024-03-09T13:42:38Z |
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| last_indexed | 2024-03-09T13:42:38Z |
| publishDate | 2022-03-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-c3c0a346d2bf4378a3ddb012bf5affb82023-11-30T21:04:50ZengMDPI AGJournal of Fungi2309-608X2022-03-018325410.3390/jof8030254Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626Margarida Palma0Stephen Mondo1Mariana Pereira2Érica Vieira3Igor V. Grigoriev4Isabel Sá-Correia5Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USAInstitute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalInstitute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USAInstitute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, PortugalThe ascomycetous yeast <i>Candida membranifaciens</i> has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated four <i>C. membranifaciens</i> strains from soil and investigated their potential to overproduce riboflavin. <i>C. membranifaciens</i> IST 626 was found to produce the highest concentrations of riboflavin. The volumetric production of this vitamin was higher when <i>C. membranifaciens</i> IST 626 cells were cultured in a commercial medium without iron and when xylose was the available carbon source compared to the same basal medium with glucose. Supplementation of the growth medium with 2 g/L glycine favored the metabolization of xylose, leading to biomass increase and consequent enhancement of riboflavin volumetric production that reached 120 mg/L after 216 h of cultivation. To gain new insights into the molecular basis of riboflavin production and carbon source utilization in this species, the first annotated genome sequence of <i>C. membranifaciens</i> is reported in this article, as well as the result of a comparative genomic analysis with other relevant yeast species. A total of 5619 genes were predicted to be present in <i>C. membranifaciens</i> IST 626 genome sequence (11.5 Mbp). Among them are genes involved in riboflavin biosynthesis, iron homeostasis, and sugar uptake and metabolism. This work put forward <i>C. membranifaciens</i> IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species.https://www.mdpi.com/2309-608X/8/3/254<i>Candida membranifaciens</i>Debaryomycetaceaeyeast isolationsoilriboflavinflavinogenic yeasts |
| spellingShingle | Margarida Palma Stephen Mondo Mariana Pereira Érica Vieira Igor V. Grigoriev Isabel Sá-Correia Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 Journal of Fungi <i>Candida membranifaciens</i> Debaryomycetaceae yeast isolation soil riboflavin flavinogenic yeasts |
| title | Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 |
| title_full | Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 |
| title_fullStr | Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 |
| title_full_unstemmed | Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 |
| title_short | Genome Sequence and Analysis of the Flavinogenic Yeast <i>Candida membranifaciens</i> IST 626 |
| title_sort | genome sequence and analysis of the flavinogenic yeast i candida membranifaciens i ist 626 |
| topic | <i>Candida membranifaciens</i> Debaryomycetaceae yeast isolation soil riboflavin flavinogenic yeasts |
| url | https://www.mdpi.com/2309-608X/8/3/254 |
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