Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a
ABSTRACT: Several strategies have been in place in food animal production to reduce the unnecessary use of antimicrobial agents. Beyond the monitoring of their use, the evaluation of the effect of these strategies on the occurrence and types of antimicrobial resistance (AMR) associated genes is cruc...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912200075X |
| _version_ | 1811280318142873600 |
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| author | Lucia Gambi Cecilia Crippa Alex Lucchi Alessandra De Cesare Antonio Parisi Gerardo Manfreda Frédérique Pasquali |
| author_facet | Lucia Gambi Cecilia Crippa Alex Lucchi Alessandra De Cesare Antonio Parisi Gerardo Manfreda Frédérique Pasquali |
| author_sort | Lucia Gambi |
| collection | DOAJ |
| description | ABSTRACT: Several strategies have been in place in food animal production to reduce the unnecessary use of antimicrobial agents. Beyond the monitoring of their use, the evaluation of the effect of these strategies on the occurrence and types of antimicrobial resistance (AMR) associated genes is crucial to untangle the potential emergence and spread of AMR to humans through the food chain. In the present study, the occurrence of these genes was evaluated in commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics” in 3 antibiotic-free (AB-free) farms in Italy in 2019. Sequenced data were analyzed along with publicly available genomes of E. coli collected in Italy from the broiler food chain from previous years (2017–2018). The genetic relationships among all 93 genomes were assessed on de novo assemblies by in silico MLST and SNP calling. Moreover, the resistomes of all genomes were investigated. According to SNP calling, genomes were gathered in three clades. Clade A encompassed, among others, ST117, ST8070, and ST1011 genomes. ST10 belonged to clade B, whereas Clade C included ST58, ST297, ST1101, and ST23 among others. Regarding the occurrence of AMR genes, a statistically significant lower occurrence of these genes in the genomes of this study in comparison to the public genomes was observed considering the whole group of genes as well as genes specifically conferring resistance to aminoglycosides, β-lactams, phenicols, trimethoprim, and lincosamides. Moreover, significant reductions were observed by comparing the whole group of AMR associated mutations, as well as those specifically for fluoroquinolones and fosfomycin resistance. Although the identification of 3° generation cephalosporin resistance associated genes in AB-free E. coli is a concern, this study provides a first indication of the impact of a more prudent use of antimicrobial agents on the occurrence of AMR genes in Italian broiler production chain. More studies are needed in next years on a higher number of genomes to confirm this preliminary observation. |
| first_indexed | 2024-04-13T01:11:28Z |
| format | Article |
| id | doaj.art-c3c0ca8622b34bac8fb5bee2bdabbecc |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-13T01:11:28Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c3c0ca8622b34bac8fb5bee2bdabbecc2022-12-22T03:09:09ZengElsevierPoultry Science0032-57912022-04-011014101770Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”aLucia Gambi0Cecilia Crippa1Alex Lucchi2Alessandra De Cesare3Antonio Parisi4Gerardo Manfreda5Frédérique Pasquali6Department of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna, 40127, ItalyDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna, 40127, ItalyDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna, 40127, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum – University of Bologna, 40064, ItalyIstituto Zooprofilattico Sperimentale della Puglia e Basilicata, Putignano (Bari), 70017, ItalyDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna, 40127, ItalyDepartment of Agricultural and Food Sciences, Alma Mater Studiorum – University of Bologna, 40127, Italy; Corresponding author:ABSTRACT: Several strategies have been in place in food animal production to reduce the unnecessary use of antimicrobial agents. Beyond the monitoring of their use, the evaluation of the effect of these strategies on the occurrence and types of antimicrobial resistance (AMR) associated genes is crucial to untangle the potential emergence and spread of AMR to humans through the food chain. In the present study, the occurrence of these genes was evaluated in commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics” in 3 antibiotic-free (AB-free) farms in Italy in 2019. Sequenced data were analyzed along with publicly available genomes of E. coli collected in Italy from the broiler food chain from previous years (2017–2018). The genetic relationships among all 93 genomes were assessed on de novo assemblies by in silico MLST and SNP calling. Moreover, the resistomes of all genomes were investigated. According to SNP calling, genomes were gathered in three clades. Clade A encompassed, among others, ST117, ST8070, and ST1011 genomes. ST10 belonged to clade B, whereas Clade C included ST58, ST297, ST1101, and ST23 among others. Regarding the occurrence of AMR genes, a statistically significant lower occurrence of these genes in the genomes of this study in comparison to the public genomes was observed considering the whole group of genes as well as genes specifically conferring resistance to aminoglycosides, β-lactams, phenicols, trimethoprim, and lincosamides. Moreover, significant reductions were observed by comparing the whole group of AMR associated mutations, as well as those specifically for fluoroquinolones and fosfomycin resistance. Although the identification of 3° generation cephalosporin resistance associated genes in AB-free E. coli is a concern, this study provides a first indication of the impact of a more prudent use of antimicrobial agents on the occurrence of AMR genes in Italian broiler production chain. More studies are needed in next years on a higher number of genomes to confirm this preliminary observation.http://www.sciencedirect.com/science/article/pii/S003257912200075Xantimicrobial resistance genesantibiotic-freecommensal Escherichia colibroiler carcasses |
| spellingShingle | Lucia Gambi Cecilia Crippa Alex Lucchi Alessandra De Cesare Antonio Parisi Gerardo Manfreda Frédérique Pasquali Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a Poultry Science antimicrobial resistance genes antibiotic-free commensal Escherichia coli broiler carcasses |
| title | Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a |
| title_full | Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a |
| title_fullStr | Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a |
| title_full_unstemmed | Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a |
| title_short | Research note: The resistome of commensal Escherichia coli isolated from broiler carcasses “produced without the use of antibiotics”a |
| title_sort | research note the resistome of commensal escherichia coli isolated from broiler carcasses produced without the use of antibiotics a |
| topic | antimicrobial resistance genes antibiotic-free commensal Escherichia coli broiler carcasses |
| url | http://www.sciencedirect.com/science/article/pii/S003257912200075X |
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