Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism.
Alginates are a class of biopolymers with known iron binding properties which are routinely used in the fabrication of iron-oxide nanoparticles. In addition, alginates have been implicated in influencing human iron absorption. However, the synthesis of iron oxide nanoparticles employs non-physiologi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0138240 |
| _version_ | 1818737384707063808 |
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| author | Richard D Horniblow Miriam Dowle Tariq H Iqbal Gladys O Latunde-Dada Richard E Palmer Zoe Pikramenou Chris Tselepis |
| author_facet | Richard D Horniblow Miriam Dowle Tariq H Iqbal Gladys O Latunde-Dada Richard E Palmer Zoe Pikramenou Chris Tselepis |
| author_sort | Richard D Horniblow |
| collection | DOAJ |
| description | Alginates are a class of biopolymers with known iron binding properties which are routinely used in the fabrication of iron-oxide nanoparticles. In addition, alginates have been implicated in influencing human iron absorption. However, the synthesis of iron oxide nanoparticles employs non-physiological pH conditions and whether nanoparticle formation in vivo is responsible for influencing cellular iron metabolism is unclear. Thus the aims of this study were to determine how alginate and iron interact at gastric-comparable pH conditions and how this influences iron metabolism. Employing a range of spectroscopic techniques under physiological conditions alginate-iron complexation was confirmed and, in conjunction with aberration corrected scanning transmission electron microscopy, nanoparticles were observed. The results infer a nucleation-type model of iron binding whereby alginate is templating the condensation of iron-hydroxide complexes to form iron oxide centred nanoparticles. The interaction of alginate and iron at a cellular level was found to decrease cellular iron acquisition by 37% (p < 0.05) and in combination with confocal microscopy the alginate inhibits cellular iron transport through extracellular iron chelation with the resulting complexes not internalised. These results infer alginate as being useful in the chelation of excess iron, especially in the context of inflammatory bowel disease and colorectal cancer where excess unabsorbed luminal iron is thought to be a driver of disease. |
| first_indexed | 2024-12-18T00:52:12Z |
| format | Article |
| id | doaj.art-c3c28604c7014766b89aa7a451867060 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T00:52:12Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c3c28604c7014766b89aa7a4518670602022-12-21T21:26:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013824010.1371/journal.pone.0138240Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism.Richard D HorniblowMiriam DowleTariq H IqbalGladys O Latunde-DadaRichard E PalmerZoe PikramenouChris TselepisAlginates are a class of biopolymers with known iron binding properties which are routinely used in the fabrication of iron-oxide nanoparticles. In addition, alginates have been implicated in influencing human iron absorption. However, the synthesis of iron oxide nanoparticles employs non-physiological pH conditions and whether nanoparticle formation in vivo is responsible for influencing cellular iron metabolism is unclear. Thus the aims of this study were to determine how alginate and iron interact at gastric-comparable pH conditions and how this influences iron metabolism. Employing a range of spectroscopic techniques under physiological conditions alginate-iron complexation was confirmed and, in conjunction with aberration corrected scanning transmission electron microscopy, nanoparticles were observed. The results infer a nucleation-type model of iron binding whereby alginate is templating the condensation of iron-hydroxide complexes to form iron oxide centred nanoparticles. The interaction of alginate and iron at a cellular level was found to decrease cellular iron acquisition by 37% (p < 0.05) and in combination with confocal microscopy the alginate inhibits cellular iron transport through extracellular iron chelation with the resulting complexes not internalised. These results infer alginate as being useful in the chelation of excess iron, especially in the context of inflammatory bowel disease and colorectal cancer where excess unabsorbed luminal iron is thought to be a driver of disease.https://doi.org/10.1371/journal.pone.0138240 |
| spellingShingle | Richard D Horniblow Miriam Dowle Tariq H Iqbal Gladys O Latunde-Dada Richard E Palmer Zoe Pikramenou Chris Tselepis Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. PLoS ONE |
| title | Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. |
| title_full | Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. |
| title_fullStr | Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. |
| title_full_unstemmed | Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. |
| title_short | Alginate-Iron Speciation and Its Effect on In Vitro Cellular Iron Metabolism. |
| title_sort | alginate iron speciation and its effect on in vitro cellular iron metabolism |
| url | https://doi.org/10.1371/journal.pone.0138240 |
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