Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver

Hepatic stellate cells (HSC) are located in Disse spaces of normal rat liver. In their quiescent state they serve as a storage site for vitamin A. In fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Changes in the cell phenotype a...

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Main Authors: Alena Jiroutová, Lenka Majdiaková, Martina Čermáková, Renata Köhlerová, Jiří Kanta
Format: Article
Language:English
Published: Karolinum Press 2005-01-01
Series:Acta Medica
Subjects:
Online Access:https://actamedica.lfhk.cuni.cz/48/3/0137/
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author Alena Jiroutová
Lenka Majdiaková
Martina Čermáková
Renata Köhlerová
Jiří Kanta
author_facet Alena Jiroutová
Lenka Majdiaková
Martina Čermáková
Renata Köhlerová
Jiří Kanta
author_sort Alena Jiroutová
collection DOAJ
description Hepatic stellate cells (HSC) are located in Disse spaces of normal rat liver. In their quiescent state they serve as a storage site for vitamin A. In fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Changes in the cell phenotype are accompanied by changes in the cellular cytoskeleton. We have studied the expression of α-smooth muscle actin and intermediate filament proteins vimentin, desmin and glial fibrillary acidic protein (GFAP) by immunocytochemistry in HSC cultured for 2 or 7 days after isolation. Normal or cirrhotic rat liver was perfused with solutions of pronase and collagenase and HSC were isolated by density gradient centrifugation of the resulting cell suspension. Liver cirrhosis was produced in rats by repeated carbon tetrachloride administration. Vimentin was detected in all cells from normal and cirrhotic liver. The concentration of desmin in the cells from cirrhotic liver was slightly higher than that in normal cells and it increased with time in culture. GFAP could be detected only in normal cells 2 days after their isolation. In contrast, alpha smooth muscle actin (α-SMA) was absent from normal cells at this time but its expression was pronouced later. In most cells from cirrhotic liver this antigen was already present on the second day of culture and its expression further increased.
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spelling doaj.art-c3c368f35d53491cbbaf75ef76b3e3512022-12-22T00:43:04ZengKarolinum PressActa Medica1211-42861805-96942005-01-0148313714410.14712/18059694.2018.41Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat LiverAlena Jiroutová0Lenka Majdiaková1Martina Čermáková2Renata Köhlerová3Jiří Kanta4Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicCharles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicHepatic stellate cells (HSC) are located in Disse spaces of normal rat liver. In their quiescent state they serve as a storage site for vitamin A. In fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells. Changes in the cell phenotype are accompanied by changes in the cellular cytoskeleton. We have studied the expression of α-smooth muscle actin and intermediate filament proteins vimentin, desmin and glial fibrillary acidic protein (GFAP) by immunocytochemistry in HSC cultured for 2 or 7 days after isolation. Normal or cirrhotic rat liver was perfused with solutions of pronase and collagenase and HSC were isolated by density gradient centrifugation of the resulting cell suspension. Liver cirrhosis was produced in rats by repeated carbon tetrachloride administration. Vimentin was detected in all cells from normal and cirrhotic liver. The concentration of desmin in the cells from cirrhotic liver was slightly higher than that in normal cells and it increased with time in culture. GFAP could be detected only in normal cells 2 days after their isolation. In contrast, alpha smooth muscle actin (α-SMA) was absent from normal cells at this time but its expression was pronouced later. In most cells from cirrhotic liver this antigen was already present on the second day of culture and its expression further increased.https://actamedica.lfhk.cuni.cz/48/3/0137/Liver cirrhosisCarbon tetrachlorideHepatic stellate cellsVimentinDesminGlial fibrillary acidic protein
spellingShingle Alena Jiroutová
Lenka Majdiaková
Martina Čermáková
Renata Köhlerová
Jiří Kanta
Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
Acta Medica
Liver cirrhosis
Carbon tetrachloride
Hepatic stellate cells
Vimentin
Desmin
Glial fibrillary acidic protein
title Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
title_full Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
title_fullStr Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
title_full_unstemmed Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
title_short Expression of Cytoskeletal Proteins in Hepatic Stellatecells Isolated from Normal and Cirrhotic Rat Liver
title_sort expression of cytoskeletal proteins in hepatic stellatecells isolated from normal and cirrhotic rat liver
topic Liver cirrhosis
Carbon tetrachloride
Hepatic stellate cells
Vimentin
Desmin
Glial fibrillary acidic protein
url https://actamedica.lfhk.cuni.cz/48/3/0137/
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