Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye
Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich ph...
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PeerJ Inc.
2022-11-01
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| author | Zhiqi Gao Lan Yun Zhen Li Qiyu Liu Chen Zhang Yingmei Ma Fengling Shi |
| author_facet | Zhiqi Gao Lan Yun Zhen Li Qiyu Liu Chen Zhang Yingmei Ma Fengling Shi |
| author_sort | Zhiqi Gao |
| collection | DOAJ |
| description | Russian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich phenotypic and genetic diversity and has potential for improvement through crossbreeding. In this study, fifteen Russian wildrye hybrid combinations were produced and one F1 population with 123 putative hybrids was obtained by crossing two individual plants with significant differences in nutritional characteristics and reproductive tiller number. Twelve phenotypic traits of the F1 population were measured for three consecutive years, and ten of the twelve traits were in line with the genetic characteristics of quantitative traits. Hybrid superiority was revealed among F1 hybrids in both nutritional and reproductive traits. One non-recurrent parent plant with the highest PCA-synthesis score was selected and used to make a backcross with the ‘BOZOISKY SELECT’ male parent, and 143 putative BC1 hybrids were obtained. Sixteen pairs of EST-SSR primers were randomly selected from polymorphic primers derived from different expressed tiller trait related genes. Three primer pairs that amplified both the paternal and maternal characteristic band were used to assess the purity of the F1 population, and three primer pairs (with one shared primer pair) were used to identify the BC1 population. The hybrid purity was 96.75% for the F1 population and 95.80% for the BC1 population, and the results were confirmed by self-fertility test through bagging isolation. The genetic similarity coefficients between the F1 progeny and the male parent ranged from 0.500 to 0.895, and those between the BC1 progeny and the male parent ranged from 0.667 to 0.939. A subset of individuals in the BC1 population had closer genetic distance to the recurrent parent, and genetic variation within the BC1 population decreased compared to the F1 population. |
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| last_indexed | 2024-03-09T07:56:00Z |
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| spelling | doaj.art-c3ce89d0eb2d4756af707d059e59f9e82023-12-03T01:01:04ZengPeerJ Inc.PeerJ2167-83592022-11-0110e1444210.7717/peerj.14442Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildryeZhiqi Gao0Lan Yun1Zhen Li2Qiyu Liu3Chen Zhang4Yingmei Ma5Fengling Shi6College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Animal Science and Technology, Hebei North University, Zhangjiakou, ChinaCollege of Desert Control Science and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollege of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaRussian wildrye, Psathyrostachys junceus (Fisch.) Nevski, is widely distributed in the high latitude areas of Eurasia. It plays an important role in grassland ecosystem maintenance, as well as being a valuable palatable forage species for livestock and wildlife. Russian wildrye germplasm has rich phenotypic and genetic diversity and has potential for improvement through crossbreeding. In this study, fifteen Russian wildrye hybrid combinations were produced and one F1 population with 123 putative hybrids was obtained by crossing two individual plants with significant differences in nutritional characteristics and reproductive tiller number. Twelve phenotypic traits of the F1 population were measured for three consecutive years, and ten of the twelve traits were in line with the genetic characteristics of quantitative traits. Hybrid superiority was revealed among F1 hybrids in both nutritional and reproductive traits. One non-recurrent parent plant with the highest PCA-synthesis score was selected and used to make a backcross with the ‘BOZOISKY SELECT’ male parent, and 143 putative BC1 hybrids were obtained. Sixteen pairs of EST-SSR primers were randomly selected from polymorphic primers derived from different expressed tiller trait related genes. Three primer pairs that amplified both the paternal and maternal characteristic band were used to assess the purity of the F1 population, and three primer pairs (with one shared primer pair) were used to identify the BC1 population. The hybrid purity was 96.75% for the F1 population and 95.80% for the BC1 population, and the results were confirmed by self-fertility test through bagging isolation. The genetic similarity coefficients between the F1 progeny and the male parent ranged from 0.500 to 0.895, and those between the BC1 progeny and the male parent ranged from 0.667 to 0.939. A subset of individuals in the BC1 population had closer genetic distance to the recurrent parent, and genetic variation within the BC1 population decreased compared to the F1 population.https://peerj.com/articles/14442.pdfPsathyrostachys junceaEST-SSR markersHybrid identificationGenetic diversityPhenotypic traitsHybrid superiority |
| spellingShingle | Zhiqi Gao Lan Yun Zhen Li Qiyu Liu Chen Zhang Yingmei Ma Fengling Shi Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye PeerJ Psathyrostachys juncea EST-SSR markers Hybrid identification Genetic diversity Phenotypic traits Hybrid superiority |
| title | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye |
| title_full | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye |
| title_fullStr | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye |
| title_full_unstemmed | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye |
| title_short | Hybrid purity identification using EST-SSR markers and heterosis analysis of quantitative traits of Russian wildrye |
| title_sort | hybrid purity identification using est ssr markers and heterosis analysis of quantitative traits of russian wildrye |
| topic | Psathyrostachys juncea EST-SSR markers Hybrid identification Genetic diversity Phenotypic traits Hybrid superiority |
| url | https://peerj.com/articles/14442.pdf |
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