Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island
Yellow leaf curl disease in chili pepper has been reported in Bali Island since the early 2012. Research was conducted to identify the virus causing this disease and disease distribution in Bali. Field survey was carried out to observe disease intensity and to collect field samples from several chi...
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| Format: | Article |
| Language: | Indonesian |
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Perhimpunan Fitopatologi Indonesia
2022-03-01
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| Series: | Jurnal Fitopatologi Indonesia |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/jfiti/article/view/37226 |
| _version_ | 1797962677962670080 |
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| author | Dewa Gede Wiryangga Selangga Suryo Wiyono Anas Dinurrohman Susila Sri Hendrastuti Hidayat |
| author_facet | Dewa Gede Wiryangga Selangga Suryo Wiyono Anas Dinurrohman Susila Sri Hendrastuti Hidayat |
| author_sort | Dewa Gede Wiryangga Selangga |
| collection | DOAJ |
| description |
Yellow leaf curl disease in chili pepper has been reported in Bali Island since the early 2012. Research was conducted to identify the virus causing this disease and disease distribution in Bali. Field survey was carried out to observe disease intensity and to collect field samples from several chili pepper growing areas in Bali (Karangasem, Bangli, Tabanan, and Gianyar). Begomovirus identification from field samples was then conducted by polymerase chain reaction method using universal primers SPG1/SPG2, followed by an analysis of the amplified target DNA sequences. The incidence of pepper yellow leaf curl disease reached 100% at all sites and disease severity reached 18%−87%. Begomovirus specific DNA fragment measuring 912 bp was successfully amplified from 12 field samples. Sequence analysis of DNA fragments showed the highest homology with Pepper yellow leaf curl Indonesia virus (PYLCIV). Further phylogenetic analysis confirmed the relationship between PYLCIV isolates from Bali and various PYLCIV isolates from Indonesia.
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| first_indexed | 2024-04-11T01:16:39Z |
| format | Article |
| id | doaj.art-c3cf7c1c0783404cbecc0f29a6199df3 |
| institution | Directory Open Access Journal |
| issn | 0215-7950 2339-2479 |
| language | Indonesian |
| last_indexed | 2024-04-11T01:16:39Z |
| publishDate | 2022-03-01 |
| publisher | Perhimpunan Fitopatologi Indonesia |
| record_format | Article |
| series | Jurnal Fitopatologi Indonesia |
| spelling | doaj.art-c3cf7c1c0783404cbecc0f29a6199df32023-01-04T02:42:53ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792022-03-0117610.14692/jfi.17.6.217-224Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali IslandDewa Gede Wiryangga Selangga0Suryo Wiyono1Anas Dinurrohman Susila2Sri Hendrastuti Hidayat3Warmadewa UniversityDepartment of Plant Protection, IPB UniversityDepartment of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University (IPB)Department of Plant Protection, IPB University Yellow leaf curl disease in chili pepper has been reported in Bali Island since the early 2012. Research was conducted to identify the virus causing this disease and disease distribution in Bali. Field survey was carried out to observe disease intensity and to collect field samples from several chili pepper growing areas in Bali (Karangasem, Bangli, Tabanan, and Gianyar). Begomovirus identification from field samples was then conducted by polymerase chain reaction method using universal primers SPG1/SPG2, followed by an analysis of the amplified target DNA sequences. The incidence of pepper yellow leaf curl disease reached 100% at all sites and disease severity reached 18%−87%. Begomovirus specific DNA fragment measuring 912 bp was successfully amplified from 12 field samples. Sequence analysis of DNA fragments showed the highest homology with Pepper yellow leaf curl Indonesia virus (PYLCIV). Further phylogenetic analysis confirmed the relationship between PYLCIV isolates from Bali and various PYLCIV isolates from Indonesia. https://journal.ipb.ac.id/index.php/jfiti/article/view/37226disease incidencephylogenetic analysispolymerase chain reactionsequence analysisuniversal primer |
| spellingShingle | Dewa Gede Wiryangga Selangga Suryo Wiyono Anas Dinurrohman Susila Sri Hendrastuti Hidayat Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island Jurnal Fitopatologi Indonesia disease incidence phylogenetic analysis polymerase chain reaction sequence analysis universal primer |
| title | Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island |
| title_full | Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island |
| title_fullStr | Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island |
| title_full_unstemmed | Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island |
| title_short | Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island |
| title_sort | distribution and identification of pepper yellow leaf curl indonesia virus infecting chili pepper in bali island |
| topic | disease incidence phylogenetic analysis polymerase chain reaction sequence analysis universal primer |
| url | https://journal.ipb.ac.id/index.php/jfiti/article/view/37226 |
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