Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy
In this paper, the first phytochemical analysis of the ethanolic extract of <i>Daphne sericea</i> Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., α-linolenic acid (<b>1</b>), tri-linoleoyl-<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/379 |
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| author | Claudio Frezza Alessandro Venditti Daniela De Vita Fabio Sciubba Pierpaolo Tomai Marco Franceschin Mirella Di Cecco Giampiero Ciaschetti Antonella Di Sotto Annarita Stringaro Marisa Colone Alessandra Gentili Mauro Serafini Armandodoriano Bianco |
| author_facet | Claudio Frezza Alessandro Venditti Daniela De Vita Fabio Sciubba Pierpaolo Tomai Marco Franceschin Mirella Di Cecco Giampiero Ciaschetti Antonella Di Sotto Annarita Stringaro Marisa Colone Alessandra Gentili Mauro Serafini Armandodoriano Bianco |
| author_sort | Claudio Frezza |
| collection | DOAJ |
| description | In this paper, the first phytochemical analysis of the ethanolic extract of <i>Daphne sericea</i> Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., α-linolenic acid (<b>1</b>), tri-linoleoyl-<i>sn</i>-glycerol (<b>2</b>), pheophorbide <i>a</i> ethyl ester (<b>3</b>), pilloin (<b>4</b>), sinensetin (<b>5</b>), yuanhuanin (<b>6</b>), rutamontine (<b>7</b>), syringin (<b>8</b>), <i>p</i>-coumaric acid (<b>9</b>), <i>p</i>-anisic acid (<b>10</b>) and caffeic acid (<b>11</b>). To the best of our knowledge, compounds (<b>1</b>-<b>4</b>, <b>7-8</b> and <b>10</b>) were isolated from <i>D. sericea</i> for the first time during this work, whereas sinensetin (<b>5</b>) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH<sup>•</sup> and 2,2′-azino-<i>bis</i>(3-thylbenzothiazoline-6-sulfonic acid (ABTS<sup>•+</sup>) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 μg/mL and after 48–72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications. |
| first_indexed | 2024-03-09T05:45:41Z |
| format | Article |
| id | doaj.art-c3d290a484444880b67fbc821db182fa |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T05:45:41Z |
| publishDate | 2021-03-01 |
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| series | Biomolecules |
| spelling | doaj.art-c3d290a484444880b67fbc821db182fa2023-12-03T12:21:25ZengMDPI AGBiomolecules2218-273X2021-03-0111337910.3390/biom11030379Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central ItalyClaudio Frezza0Alessandro Venditti1Daniela De Vita2Fabio Sciubba3Pierpaolo Tomai4Marco Franceschin5Mirella Di Cecco6Giampiero Ciaschetti7Antonella Di Sotto8Annarita Stringaro9Marisa Colone10Alessandra Gentili11Mauro Serafini12Armandodoriano Bianco13Dipartimento di Biologia Ambientale, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Biologia Ambientale, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyUfficio Monitoraggio e Conservazione della Biodiversità Vegetale, Parco Nazionale della Majella, Via Badia 28, 67039 Sulmona, ItalyUfficio Monitoraggio e Conservazione della Biodiversità Vegetale, Parco Nazionale della Majella, Via Badia 28, 67039 Sulmona, ItalyDipartimento di Fisiologia e Farmacologia “V. Erspamer”, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyCentro Nazionale per la Ricerca e la Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyCentro Nazionale per la Ricerca e la Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Biologia Ambientale, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyDipartimento di Chimica, Università di Roma “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, ItalyIn this paper, the first phytochemical analysis of the ethanolic extract of <i>Daphne sericea</i> Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., α-linolenic acid (<b>1</b>), tri-linoleoyl-<i>sn</i>-glycerol (<b>2</b>), pheophorbide <i>a</i> ethyl ester (<b>3</b>), pilloin (<b>4</b>), sinensetin (<b>5</b>), yuanhuanin (<b>6</b>), rutamontine (<b>7</b>), syringin (<b>8</b>), <i>p</i>-coumaric acid (<b>9</b>), <i>p</i>-anisic acid (<b>10</b>) and caffeic acid (<b>11</b>). To the best of our knowledge, compounds (<b>1</b>-<b>4</b>, <b>7-8</b> and <b>10</b>) were isolated from <i>D. sericea</i> for the first time during this work, whereas sinensetin (<b>5</b>) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH<sup>•</sup> and 2,2′-azino-<i>bis</i>(3-thylbenzothiazoline-6-sulfonic acid (ABTS<sup>•+</sup>) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 μg/mL and after 48–72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications.https://www.mdpi.com/2218-273X/11/3/379<i>Daphne sericea</i> VahlThymelaeaceaephytochemical analysischemotaxonomypolyphenolsantioxidant activities |
| spellingShingle | Claudio Frezza Alessandro Venditti Daniela De Vita Fabio Sciubba Pierpaolo Tomai Marco Franceschin Mirella Di Cecco Giampiero Ciaschetti Antonella Di Sotto Annarita Stringaro Marisa Colone Alessandra Gentili Mauro Serafini Armandodoriano Bianco Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy Biomolecules <i>Daphne sericea</i> Vahl Thymelaeaceae phytochemical analysis chemotaxonomy polyphenols antioxidant activities |
| title | Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy |
| title_full | Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy |
| title_fullStr | Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy |
| title_full_unstemmed | Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy |
| title_short | Phytochemical Analysis and Biological Activities of the Ethanolic Extract of <i>Daphne sericea</i> Vahl Flowering Aerial Parts Collected in Central Italy |
| title_sort | phytochemical analysis and biological activities of the ethanolic extract of i daphne sericea i vahl flowering aerial parts collected in central italy |
| topic | <i>Daphne sericea</i> Vahl Thymelaeaceae phytochemical analysis chemotaxonomy polyphenols antioxidant activities |
| url | https://www.mdpi.com/2218-273X/11/3/379 |
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