Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries

The aim of this study was to isolate, genetically characterize and determine phylogenetic relationship of strains detected in Serbia in comparison to other strains. A total of 480 samples collected from 13 different commercial layer flocks, obtained from tracheal swabs were included. Samples taken f...

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Main Authors: Bojana VIDOVIĆ, Milanko ŠEKLER, Dragan ROGAN, Dejan VIDANOVIĆ, Gordana NEDELJKOVIĆ, Aleksandar POTKONJAK, Miodrag RADINOVIĆ, Nikolina NOVAKOV
Format: Article
Language:English
Published: Kafkas University, Faculty of Veterinary Medicine 2018-09-01
Series:Kafkas Universitesi Veteriner Fakültesi Dergisi
Subjects:
Online Access:https://vetdergikafkas.org/pdf.php?id=2416
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author Bojana VIDOVIĆ
Milanko ŠEKLER
Dragan ROGAN
Dejan VIDANOVIĆ
Gordana NEDELJKOVIĆ
Aleksandar POTKONJAK
Miodrag RADINOVIĆ
Nikolina NOVAKOV
author_facet Bojana VIDOVIĆ
Milanko ŠEKLER
Dragan ROGAN
Dejan VIDANOVIĆ
Gordana NEDELJKOVIĆ
Aleksandar POTKONJAK
Miodrag RADINOVIĆ
Nikolina NOVAKOV
author_sort Bojana VIDOVIĆ
collection DOAJ
description The aim of this study was to isolate, genetically characterize and determine phylogenetic relationship of strains detected in Serbia in comparison to other strains. A total of 480 samples collected from 13 different commercial layer flocks, obtained from tracheal swabs were included. Samples taken from 2016 to 2017 were molecularly analyzed by real-time RT-PCR, multiplex nested RT-PCR, and by sequencing of the S1 gene. Phylogenetic analyses based on partial S1 sequences revealed that six strains were classified as the D274 genotype, two strains as the QX genotype and two strains as the 4/91 genotype. The difference in nucleotide similarity between the Serbian isolates belonging to the D274 group ranges from 0 to 1.2%. Comparison of the obtained strains and D274 (X15832) showed differences from 0 to 0.9%. The greatest nucleotide similarity of detected QX strains was with Chinese QXIBV (KC795604), ranging from 98.8% to 99.1%. Two Serbian strains belonging to the 4/91 genotype had 99.7% and 98.8% nucleotide similarities with vaccine strain 4/91 (KF377577). This study has shown that viruses belonging to D274, QX, and 4/91 genotypes were circulating in poultry flocks in Serbia during 2016 and 2017.
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spelling doaj.art-c3d3449d98b94ce187a247767de270d22023-06-19T06:41:19ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-09-0124688188610.9775/kvfd.2018.202012416Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countriesBojana VIDOVIĆ0Milanko ŠEKLER1Dragan ROGAN2Dejan VIDANOVIĆ3Gordana NEDELJKOVIĆ4Aleksandar POTKONJAK5Miodrag RADINOVIĆ6Nikolina NOVAKOV7Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SERBIADepartment of Laboratory Diagnostic, Veterinary Specialist Institute, Žička 34, 36000 Kraljevo, SERBIADepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SERBIADepartment of Laboratory Diagnostic, Veterinary Specialist Institute, Žička 34, 36000 Kraljevo, SERBIADepartment of Poultry Diseases with a Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, CROATIADepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SERBIADepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SERBIADepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SERBIAThe aim of this study was to isolate, genetically characterize and determine phylogenetic relationship of strains detected in Serbia in comparison to other strains. A total of 480 samples collected from 13 different commercial layer flocks, obtained from tracheal swabs were included. Samples taken from 2016 to 2017 were molecularly analyzed by real-time RT-PCR, multiplex nested RT-PCR, and by sequencing of the S1 gene. Phylogenetic analyses based on partial S1 sequences revealed that six strains were classified as the D274 genotype, two strains as the QX genotype and two strains as the 4/91 genotype. The difference in nucleotide similarity between the Serbian isolates belonging to the D274 group ranges from 0 to 1.2%. Comparison of the obtained strains and D274 (X15832) showed differences from 0 to 0.9%. The greatest nucleotide similarity of detected QX strains was with Chinese QXIBV (KC795604), ranging from 98.8% to 99.1%. Two Serbian strains belonging to the 4/91 genotype had 99.7% and 98.8% nucleotide similarities with vaccine strain 4/91 (KF377577). This study has shown that viruses belonging to D274, QX, and 4/91 genotypes were circulating in poultry flocks in Serbia during 2016 and 2017.https://vetdergikafkas.org/pdf.php?id=2416ibvgenotypingqxd2744/91
spellingShingle Bojana VIDOVIĆ
Milanko ŠEKLER
Dragan ROGAN
Dejan VIDANOVIĆ
Gordana NEDELJKOVIĆ
Aleksandar POTKONJAK
Miodrag RADINOVIĆ
Nikolina NOVAKOV
Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
Kafkas Universitesi Veteriner Fakültesi Dergisi
ibv
genotyping
qx
d274
4/91
title Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
title_full Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
title_fullStr Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
title_full_unstemmed Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
title_short Molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
title_sort molecular characterization of infectious bronchitis virus strains isolated from vaccinated flocks in serbia and their comparison with the isolated strains from neighboring countries
topic ibv
genotyping
qx
d274
4/91
url https://vetdergikafkas.org/pdf.php?id=2416
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