Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India

In the present study, sludge samples were collected from the place where effluents were released by the carpet industries in Bhadohi district. Physico-chemical analysis revealed sludge samples were dark brown in color, pH (6.91 to 9.5), ECe (5.69-11.50 dSm-1), organic C (4.40 - 12.60%), N content (3...

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Main Authors: Garima Singh, Sudhir K. Upadhyay, M. P. Singh
Format: Article
Language:English
Published: Environment, Agriculture and Education Society 2015-06-01
Series:G-Journal of Environmental Science and Technology
Subjects:
Online Access:https://gjestenv.com/index.php/gjest/article/view/43
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author Garima Singh
Sudhir K. Upadhyay
M. P. Singh
author_facet Garima Singh
Sudhir K. Upadhyay
M. P. Singh
author_sort Garima Singh
collection DOAJ
description In the present study, sludge samples were collected from the place where effluents were released by the carpet industries in Bhadohi district. Physico-chemical analysis revealed sludge samples were dark brown in color, pH (6.91 to 9.5), ECe (5.69-11.50 dSm-1), organic C (4.40 - 12.60%), N content (3.50 -13.90%), PO4 content (7.25-13.34%) and K2O content (1.02 -3.21%). Maximum population of bacterial (cfu≈106/g) isolates in sludge samples were found on NA media followed by Kings’ B and NMS. Out of 86 bacterial isolates, 39, 28 and 19 isolates were isolated on NA, Kings’ B and NMS agar media respectively. Sixteen bacterial isolates decolorized the test dyes in respective media plates, 10 decolorized congo red (CR) and 6 decolorized trypan blue (TB) up to higher concentration (25 to 150 ppm) of dyes in agar plate. Pseudomonas sp., Bacillus sp. and Azatobacter sp. and their consortium showed significant decororization of both the dyes after 24h in Nutrient broth. Consortium of C3 (Bacillus sp. + Azatobacter sp.) decolorized 94% of 100 ppm CR and 95% of TB.
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spelling doaj.art-c3d440d548a54f6581a703f21617e2fb2023-09-03T10:38:32ZengEnvironment, Agriculture and Education SocietyG-Journal of Environmental Science and Technology2322-02282015-06-0126Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- IndiaGarima Singh0Sudhir K. Upadhyay1M. P. Singh2Department of Environmental Science, V.B.S. Purvanchal University, Jaunpur, U.P. INDIADepartment of Environmental Science, V.B.S. Purvanchal University, Jaunpur, U.P. INDIACentre of Biotechnology, University of Allahabad, Allahabad, U.P. INDIAIn the present study, sludge samples were collected from the place where effluents were released by the carpet industries in Bhadohi district. Physico-chemical analysis revealed sludge samples were dark brown in color, pH (6.91 to 9.5), ECe (5.69-11.50 dSm-1), organic C (4.40 - 12.60%), N content (3.50 -13.90%), PO4 content (7.25-13.34%) and K2O content (1.02 -3.21%). Maximum population of bacterial (cfu≈106/g) isolates in sludge samples were found on NA media followed by Kings’ B and NMS. Out of 86 bacterial isolates, 39, 28 and 19 isolates were isolated on NA, Kings’ B and NMS agar media respectively. Sixteen bacterial isolates decolorized the test dyes in respective media plates, 10 decolorized congo red (CR) and 6 decolorized trypan blue (TB) up to higher concentration (25 to 150 ppm) of dyes in agar plate. Pseudomonas sp., Bacillus sp. and Azatobacter sp. and their consortium showed significant decororization of both the dyes after 24h in Nutrient broth. Consortium of C3 (Bacillus sp. + Azatobacter sp.) decolorized 94% of 100 ppm CR and 95% of TB.https://gjestenv.com/index.php/gjest/article/view/43Azo-dyeTB (Trypan blue)CR (congo red)Native bacteriaDecolorization
spellingShingle Garima Singh
Sudhir K. Upadhyay
M. P. Singh
Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
G-Journal of Environmental Science and Technology
Azo-dye
TB (Trypan blue)
CR (congo red)
Native bacteria
Decolorization
title Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
title_full Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
title_fullStr Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
title_full_unstemmed Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
title_short Dye-decolorization by native bacterial isolates, isolated from sludge of carpet industries Bhadohi- India
title_sort dye decolorization by native bacterial isolates isolated from sludge of carpet industries bhadohi india
topic Azo-dye
TB (Trypan blue)
CR (congo red)
Native bacteria
Decolorization
url https://gjestenv.com/index.php/gjest/article/view/43
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AT sudhirkupadhyay dyedecolorizationbynativebacterialisolatesisolatedfromsludgeofcarpetindustriesbhadohiindia
AT mpsingh dyedecolorizationbynativebacterialisolatesisolatedfromsludgeofcarpetindustriesbhadohiindia