Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids

Tear glucose is an intriguing biofluid that includes potential biomarkers. While many sensors have emerged nowadays, there is still demand for advanced sensors with nonenzymatic, simple, cost-effective sensing mechanism. Herein, we present a paper-based colorimetric assay by utilizing a gold nanopar...

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Main Authors: Hyeok Jung Kim, Jiwoo Hyung, Hyeran Noh
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Chemosensors
Subjects:
Online Access:https://www.mdpi.com/2227-9040/8/3/81
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author Hyeok Jung Kim
Jiwoo Hyung
Hyeran Noh
author_facet Hyeok Jung Kim
Jiwoo Hyung
Hyeran Noh
author_sort Hyeok Jung Kim
collection DOAJ
description Tear glucose is an intriguing biofluid that includes potential biomarkers. While many sensors have emerged nowadays, there is still demand for advanced sensors with nonenzymatic, simple, cost-effective sensing mechanism. Herein, we present a paper-based colorimetric assay by utilizing a gold nanoparticle formation. Experimental characterization substantiated a mechanism in this in situ reduction. Scanning electron microscopy, UV-visible spectrometry, etc. were involved in the scrutiny. As a result, we reached for the rationale whereas the particle formation can be utilized for a glucose sensing using tears. This paper-based detection was insusceptible to physiological tear matrix, i.e., chloride ion effect, false-positive error and synergistic effect by antioxidants. In addition, we evaluated its analytical performance in an artificial tear assay. Of the obtained a linear regressions, the concentration range corresponded to the physiological or pathologic reference range. In addition, within the low-concentration range, a high correlation was resulted 0.965. Furthermore, we investigated statistical validation by employing the Bland–Altman plot. In the end sections of this paper, we denoted its ready-to-use merits by simplicity—as well as the further application of our plasmonic paper.
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spelling doaj.art-c3d5ed5601874570a32569604973eecf2023-11-20T12:08:33ZengMDPI AGChemosensors2227-90402020-09-01838110.3390/chemosensors8030081Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular FluidsHyeok Jung Kim0Jiwoo Hyung1Hyeran Noh2Department of Optometry, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, KoreaDepartment of Optometry, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, KoreaDepartment of Optometry, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811, KoreaTear glucose is an intriguing biofluid that includes potential biomarkers. While many sensors have emerged nowadays, there is still demand for advanced sensors with nonenzymatic, simple, cost-effective sensing mechanism. Herein, we present a paper-based colorimetric assay by utilizing a gold nanoparticle formation. Experimental characterization substantiated a mechanism in this in situ reduction. Scanning electron microscopy, UV-visible spectrometry, etc. were involved in the scrutiny. As a result, we reached for the rationale whereas the particle formation can be utilized for a glucose sensing using tears. This paper-based detection was insusceptible to physiological tear matrix, i.e., chloride ion effect, false-positive error and synergistic effect by antioxidants. In addition, we evaluated its analytical performance in an artificial tear assay. Of the obtained a linear regressions, the concentration range corresponded to the physiological or pathologic reference range. In addition, within the low-concentration range, a high correlation was resulted 0.965. Furthermore, we investigated statistical validation by employing the Bland–Altman plot. In the end sections of this paper, we denoted its ready-to-use merits by simplicity—as well as the further application of our plasmonic paper.https://www.mdpi.com/2227-9040/8/3/81gold nanoparticlesnanocompositeglucosetearplasmonicpaper-based
spellingShingle Hyeok Jung Kim
Jiwoo Hyung
Hyeran Noh
Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
Chemosensors
gold nanoparticles
nanocomposite
glucose
tear
plasmonic
paper-based
title Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
title_full Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
title_fullStr Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
title_full_unstemmed Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
title_short Rationalization of In-Situ Synthesized Plasmonic Paper for Colorimetric Detection of Glucose in Ocular Fluids
title_sort rationalization of in situ synthesized plasmonic paper for colorimetric detection of glucose in ocular fluids
topic gold nanoparticles
nanocomposite
glucose
tear
plasmonic
paper-based
url https://www.mdpi.com/2227-9040/8/3/81
work_keys_str_mv AT hyeokjungkim rationalizationofinsitusynthesizedplasmonicpaperforcolorimetricdetectionofglucoseinocularfluids
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AT hyerannoh rationalizationofinsitusynthesizedplasmonicpaperforcolorimetricdetectionofglucoseinocularfluids