Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model
Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis.Many natural health products inhibit angiogenesis. Because of the lack of molecular studieson anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinicalstudies on other shallot p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2009-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/Article12.pdf |
| Summary: | Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis.Many natural health products inhibit angiogenesis. Because of the lack of molecular studieson anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinicalstudies on other shallot properties, such as the anti-proliferative effect of shallot chloroformicextract on two tumor cell lines, the present study focuses on the anti-angiogenic effect ofaqueous shallot extract using an aorta ring model.Materials and Methods: Aortic rings were obtained by cross-sectioning, at 1-2 mm intervals,the thoracic aorta of 4-8 weeks old Wistar male rats and cultured them in a thin drop oftype I collagen gel. After 3 days of culturing and first sprouting, the extract of A. ascalonicum(from 25 to 800 μg/ml) was added to cultures. The results of anti-angiogenic activity wereinvestigated by microscope. The cytotoxicity of extract at different doses on HUVECs wasmeasured by trypan blue assay.Results: The results showed that the shallot extract has suitable anti-angiogenic effect in arange of 50 to 800 μg/ml, but in 25 μg/ml, the extract has no considerable effect. In addition,a tangible cytotoxic effect on endothelial cells at the above mentioned doses was observed.Conclusion: Our study showed that aqueous extract of A. ascalonicum bulbs has noticeableanti-angiogenic activity without toxic effect on the cells in doses that ranged from 50-800 μg/ml. Therefore, A. ascalonicum can be a potential candidate for further investigations used inangiogenesis-related pathologic conditions. |
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| ISSN: | 2228-5806 2228-5814 |