Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model
Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis.Many natural health products inhibit angiogenesis. Because of the lack of molecular studieson anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinicalstudies on other shallot p...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2009-01-01
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| Series: | Cell Journal |
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| Online Access: | http://celljournal.org/library/upload/article/Article12.pdf |
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| author | Abbas Siami Reza Khodarahmi Maryam Keshavarz Yadollah Shakiba Kamran Mansouri Hamid Reza Mohammadi Motlagh Ali Mostafaie |
| author_facet | Abbas Siami Reza Khodarahmi Maryam Keshavarz Yadollah Shakiba Kamran Mansouri Hamid Reza Mohammadi Motlagh Ali Mostafaie |
| author_sort | Abbas Siami |
| collection | DOAJ |
| description | Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis.Many natural health products inhibit angiogenesis. Because of the lack of molecular studieson anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinicalstudies on other shallot properties, such as the anti-proliferative effect of shallot chloroformicextract on two tumor cell lines, the present study focuses on the anti-angiogenic effect ofaqueous shallot extract using an aorta ring model.Materials and Methods: Aortic rings were obtained by cross-sectioning, at 1-2 mm intervals,the thoracic aorta of 4-8 weeks old Wistar male rats and cultured them in a thin drop oftype I collagen gel. After 3 days of culturing and first sprouting, the extract of A. ascalonicum(from 25 to 800 μg/ml) was added to cultures. The results of anti-angiogenic activity wereinvestigated by microscope. The cytotoxicity of extract at different doses on HUVECs wasmeasured by trypan blue assay.Results: The results showed that the shallot extract has suitable anti-angiogenic effect in arange of 50 to 800 μg/ml, but in 25 μg/ml, the extract has no considerable effect. In addition,a tangible cytotoxic effect on endothelial cells at the above mentioned doses was observed.Conclusion: Our study showed that aqueous extract of A. ascalonicum bulbs has noticeableanti-angiogenic activity without toxic effect on the cells in doses that ranged from 50-800 μg/ml. Therefore, A. ascalonicum can be a potential candidate for further investigations used inangiogenesis-related pathologic conditions. |
| first_indexed | 2024-12-11T11:45:20Z |
| format | Article |
| id | doaj.art-c3d5f0f02b494405982acd4d7c379e91 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-11T11:45:20Z |
| publishDate | 2009-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-c3d5f0f02b494405982acd4d7c379e912022-12-22T01:08:30ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142009-01-01112190195Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring ModelAbbas SiamiReza KhodarahmiMaryam KeshavarzYadollah ShakibaKamran MansouriHamid Reza Mohammadi MotlaghAli MostafaieObjective: Angiogenesis is a key process in the promotion of cancer and its metastasis.Many natural health products inhibit angiogenesis. Because of the lack of molecular studieson anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinicalstudies on other shallot properties, such as the anti-proliferative effect of shallot chloroformicextract on two tumor cell lines, the present study focuses on the anti-angiogenic effect ofaqueous shallot extract using an aorta ring model.Materials and Methods: Aortic rings were obtained by cross-sectioning, at 1-2 mm intervals,the thoracic aorta of 4-8 weeks old Wistar male rats and cultured them in a thin drop oftype I collagen gel. After 3 days of culturing and first sprouting, the extract of A. ascalonicum(from 25 to 800 μg/ml) was added to cultures. The results of anti-angiogenic activity wereinvestigated by microscope. The cytotoxicity of extract at different doses on HUVECs wasmeasured by trypan blue assay.Results: The results showed that the shallot extract has suitable anti-angiogenic effect in arange of 50 to 800 μg/ml, but in 25 μg/ml, the extract has no considerable effect. In addition,a tangible cytotoxic effect on endothelial cells at the above mentioned doses was observed.Conclusion: Our study showed that aqueous extract of A. ascalonicum bulbs has noticeableanti-angiogenic activity without toxic effect on the cells in doses that ranged from 50-800 μg/ml. Therefore, A. ascalonicum can be a potential candidate for further investigations used inangiogenesis-related pathologic conditions.http://celljournal.org/library/upload/article/Article12.pdfAngiogenesisAllium ascalonicumAortic RingsEndothelial CellsAqueous Extract |
| spellingShingle | Abbas Siami Reza Khodarahmi Maryam Keshavarz Yadollah Shakiba Kamran Mansouri Hamid Reza Mohammadi Motlagh Ali Mostafaie Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model Cell Journal Angiogenesis Allium ascalonicum Aortic Rings Endothelial Cells Aqueous Extract |
| title | Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model |
| title_full | Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model |
| title_fullStr | Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model |
| title_full_unstemmed | Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model |
| title_short | Anti-Angiogenic Effect of Aqueous Extract of Shallot (Allium ascalonicum) Bulbs in Rat Aorta Ring Model |
| title_sort | anti angiogenic effect of aqueous extract of shallot allium ascalonicum bulbs in rat aorta ring model |
| topic | Angiogenesis Allium ascalonicum Aortic Rings Endothelial Cells Aqueous Extract |
| url | http://celljournal.org/library/upload/article/Article12.pdf |
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