MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1
Abstract Background While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism. Materials & methods MTT, wound-healing, transwell migration and transwell inva...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Cellular & Molecular Biology Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11658-019-0155-z |
| _version_ | 1818560334721449984 |
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| author | Yuxiang Fu Liewen Lin Ligang Xia |
| author_facet | Yuxiang Fu Liewen Lin Ligang Xia |
| author_sort | Yuxiang Fu |
| collection | DOAJ |
| description | Abstract Background While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism. Materials & methods MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the role of miR-107 in SW629 cell proliferation, migration and invasion. Real time-PCR and dual-luciferase reporter gene, TFR1 overexpression and western blotting assays were used to explore the underlying mechanism. Results MiR-107 is downregulated in colorectal cancer tissues and several human colorectal cancer cell lines. Low miR-107 expression often indicates a poor survival rate for colorectal cancer patients. MiR-107 suppresses the proliferation, migration and invasion of SW620 cells by negatively regulating transferrin receptor 1 (TFR1). Conclusion MiR-107 suppresses the metastasis of colorectal cancer and could be a potential therapy target in colorectal cancer patients. |
| first_indexed | 2024-12-14T00:37:10Z |
| format | Article |
| id | doaj.art-c3d6edc1f69c4795bb03c06169198042 |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-12-14T00:37:10Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-c3d6edc1f69c4795bb03c061691980422022-12-21T23:24:36ZengBMCCellular & Molecular Biology Letters1425-81531689-13922019-05-0124111110.1186/s11658-019-0155-zMiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1Yuxiang Fu0Liewen Lin1Ligang Xia2Department of Gastrointestinal Surgery, Shenzhen People’s Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of South University of Science and TechnologyDepartment of Gastrointestinal Surgery, Shenzhen People’s Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of South University of Science and TechnologyDepartment of Gastrointestinal Surgery, Shenzhen People’s Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of South University of Science and TechnologyAbstract Background While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism. Materials & methods MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the role of miR-107 in SW629 cell proliferation, migration and invasion. Real time-PCR and dual-luciferase reporter gene, TFR1 overexpression and western blotting assays were used to explore the underlying mechanism. Results MiR-107 is downregulated in colorectal cancer tissues and several human colorectal cancer cell lines. Low miR-107 expression often indicates a poor survival rate for colorectal cancer patients. MiR-107 suppresses the proliferation, migration and invasion of SW620 cells by negatively regulating transferrin receptor 1 (TFR1). Conclusion MiR-107 suppresses the metastasis of colorectal cancer and could be a potential therapy target in colorectal cancer patients.http://link.springer.com/article/10.1186/s11658-019-0155-zColorectal cancermicroRNA107 (miR-107)Cancer progressTransferrin receptor 1 (TFR1) |
| spellingShingle | Yuxiang Fu Liewen Lin Ligang Xia MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 Cellular & Molecular Biology Letters Colorectal cancer microRNA107 (miR-107) Cancer progress Transferrin receptor 1 (TFR1) |
| title | MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| title_full | MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| title_fullStr | MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| title_full_unstemmed | MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| title_short | MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| title_sort | mir 107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1 |
| topic | Colorectal cancer microRNA107 (miR-107) Cancer progress Transferrin receptor 1 (TFR1) |
| url | http://link.springer.com/article/10.1186/s11658-019-0155-z |
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