Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study
Abstract Elevated serum IgG4 is a useful marker of IgG4-related disease (IgG4-RD) activity. However, there is no uniformity in the cut-off values of IgG4 among the various reagents. The aim of this study was to compare the measured and cut-off values of IgG4 assessed using three different reagents....
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-86024-5 |
| _version_ | 1818432357324029952 |
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| author | Yoko Usami Mitsutoshi Sugano Takeshi Uehara Masayoshi Koinuma Nau Ishimine Kenji Kawasaki Kazuyoshi Yamauchi Hideaki Hamano Takayuki Honda |
| author_facet | Yoko Usami Mitsutoshi Sugano Takeshi Uehara Masayoshi Koinuma Nau Ishimine Kenji Kawasaki Kazuyoshi Yamauchi Hideaki Hamano Takayuki Honda |
| author_sort | Yoko Usami |
| collection | DOAJ |
| description | Abstract Elevated serum IgG4 is a useful marker of IgG4-related disease (IgG4-RD) activity. However, there is no uniformity in the cut-off values of IgG4 among the various reagents. The aim of this study was to compare the measured and cut-off values of IgG4 assessed using three different reagents. This study enrolled 466 IgG4-RD and non-IgG4-RD patients who required measurement of serum IgG4 levels to diagnose or treat IgG4-RD. Serum IgG4 was measured using three reagents: N-assay LA IgG4 Nittobo (Nittobo), BS-NIA IgG4 (TBS), and N Latex IgG4 (Siemens). The values obtained using the three reagents were compared, and cut-off values were calculated for each. Although there was good correlation among the results with the three reagents, the measured and cut-off values were all different. The Nittobo values were 1.4 times the TBS values and the TBS values were almost half those of the Siemens values. ROC curve analysis showed cut-off values for the Nittobo, TBS, and Siemens reagents of 1.42, 1.31, and 2.38 g/L, respectively. The measured and cut-off values of serum IgG4 vary depending on the reagents used for the assay, although there is good correlation among the values measured by the three reagents. |
| first_indexed | 2024-12-14T16:03:55Z |
| format | Article |
| id | doaj.art-c3d99f3a5cb94f7faf69d081f1d61627 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:03:55Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c3d99f3a5cb94f7faf69d081f1d616272022-12-21T22:55:08ZengNature PortfolioScientific Reports2045-23222021-03-011111710.1038/s41598-021-86024-5Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter studyYoko Usami0Mitsutoshi Sugano1Takeshi Uehara2Masayoshi Koinuma3Nau Ishimine4Kenji Kawasaki5Kazuyoshi Yamauchi6Hideaki Hamano7Takayuki Honda8Department of Laboratory Medicine, Shinshu University HospitalDepartment of Laboratory Medicine, Shinshu University HospitalDepartment of Laboratory Medicine, Shinshu University HospitalFaculty of Pharmaceutical Sciences, Teikyo Heisei UniversityDepartment of Laboratory Medicine, Shinshu University HospitalDepartment of Laboratory Medicine, Chiba University School of MedicineDepartment of Laboratory Medicine, Faculty of Medicine, University of TsukubaNagano Prefectural Kiso HospitalDepartment of Laboratory Medicine, Shinshu University HospitalAbstract Elevated serum IgG4 is a useful marker of IgG4-related disease (IgG4-RD) activity. However, there is no uniformity in the cut-off values of IgG4 among the various reagents. The aim of this study was to compare the measured and cut-off values of IgG4 assessed using three different reagents. This study enrolled 466 IgG4-RD and non-IgG4-RD patients who required measurement of serum IgG4 levels to diagnose or treat IgG4-RD. Serum IgG4 was measured using three reagents: N-assay LA IgG4 Nittobo (Nittobo), BS-NIA IgG4 (TBS), and N Latex IgG4 (Siemens). The values obtained using the three reagents were compared, and cut-off values were calculated for each. Although there was good correlation among the results with the three reagents, the measured and cut-off values were all different. The Nittobo values were 1.4 times the TBS values and the TBS values were almost half those of the Siemens values. ROC curve analysis showed cut-off values for the Nittobo, TBS, and Siemens reagents of 1.42, 1.31, and 2.38 g/L, respectively. The measured and cut-off values of serum IgG4 vary depending on the reagents used for the assay, although there is good correlation among the values measured by the three reagents.https://doi.org/10.1038/s41598-021-86024-5 |
| spellingShingle | Yoko Usami Mitsutoshi Sugano Takeshi Uehara Masayoshi Koinuma Nau Ishimine Kenji Kawasaki Kazuyoshi Yamauchi Hideaki Hamano Takayuki Honda Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study Scientific Reports |
| title | Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study |
| title_full | Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study |
| title_fullStr | Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study |
| title_full_unstemmed | Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study |
| title_short | Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study |
| title_sort | cut off values of serum igg4 among three reagents including a novel igg4 reagent a multicenter study |
| url | https://doi.org/10.1038/s41598-021-86024-5 |
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