Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils
Agriculturally-driven land transformation is increasing globally. Improving phosphorus (P) use efficiency to sustain optimum productivity in diverse ecosystems, based on knowledge of soil P dynamics, is also globally important in light of potential shortages of rock phosphate to manufacture P fertil...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01643/full |
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| author | Jin Liu Jin Liu Barbara J. Cade-Menun Jianjun Yang Yongfeng Hu Corey W. Liu Julien Tremblay Kerry LaForge Michael Schellenberg Chantal Hamel Luke D. Bainard |
| author_facet | Jin Liu Jin Liu Barbara J. Cade-Menun Jianjun Yang Yongfeng Hu Corey W. Liu Julien Tremblay Kerry LaForge Michael Schellenberg Chantal Hamel Luke D. Bainard |
| author_sort | Jin Liu |
| collection | DOAJ |
| description | Agriculturally-driven land transformation is increasing globally. Improving phosphorus (P) use efficiency to sustain optimum productivity in diverse ecosystems, based on knowledge of soil P dynamics, is also globally important in light of potential shortages of rock phosphate to manufacture P fertilizer. We investigated P chemical speciation and P cycling with solution 31P nuclear magnetic resonance, P K-edge X-ray absorption near-edge structure spectroscopy, phosphatase activity assays, and shotgun metagenomics in soil samples from long-term agricultural fields containing four different land-use types (native and tame grasslands, annual croplands, and roadside ditches). Across these land use types, native and tame grasslands showed high accumulation of organic P, principally orthophosphate monoesters, and high acid phosphomonoesterase activity but the lowest abundance of P cycling genes. The proportion of inositol hexaphosphates (IHP), especially the neo-IHP stereoisomer that likely originates from microbes rather than plants, was significantly increased in native grasslands than croplands. Annual croplands had the largest variances of soil P composition, and the highest potential capacity for P cycling processes based on the abundance of genes coding for P cycling processes. In contrast, roadside soils had the highest soil Olsen-P concentrations, lowest organic P, and highest tricalcium phosphate concentrations, which were likely facilitated by the neutral pH and high exchangeable Ca of these soils. Redundancy analysis demonstrated that IHP by NMR, potential phosphatase activity, Olsen-P, and pH were important P chemistry predictors of the P cycling bacterial community and functional gene composition. Combining chemical and metagenomics results provides important insights into soil P processes and dynamics in different land-use ecosystems. |
| first_indexed | 2024-04-13T13:34:00Z |
| format | Article |
| id | doaj.art-c3da1dae00244ffdae2db8bac79060a4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T13:34:00Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c3da1dae00244ffdae2db8bac79060a42022-12-22T02:44:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01643381167Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural SoilsJin Liu0Jin Liu1Barbara J. Cade-Menun2Jianjun Yang3Yongfeng Hu4Corey W. Liu5Julien Tremblay6Kerry LaForge7Michael Schellenberg8Chantal Hamel9Luke D. Bainard10College of Agronomy and Biotechnology, China Agricultural University, Beijing, ChinaVisiting Scientist, Agriculture and Agri-Food Canada, Swift Current Research and Development Centre, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaInstitute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, ChinaCanadian Light Source, University of Saskatchewan, Saskatoon, SK, CanadaStanford Magnetic Resonance Laboratory, Stanford University School of Medicine and ChEM-H-Stanford University, Stanford, CA, United StatesEnergy, Mining and Environment, National Research Council of Canada, Montreal, QC, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaSwift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, SK, CanadaAgriculturally-driven land transformation is increasing globally. Improving phosphorus (P) use efficiency to sustain optimum productivity in diverse ecosystems, based on knowledge of soil P dynamics, is also globally important in light of potential shortages of rock phosphate to manufacture P fertilizer. We investigated P chemical speciation and P cycling with solution 31P nuclear magnetic resonance, P K-edge X-ray absorption near-edge structure spectroscopy, phosphatase activity assays, and shotgun metagenomics in soil samples from long-term agricultural fields containing four different land-use types (native and tame grasslands, annual croplands, and roadside ditches). Across these land use types, native and tame grasslands showed high accumulation of organic P, principally orthophosphate monoesters, and high acid phosphomonoesterase activity but the lowest abundance of P cycling genes. The proportion of inositol hexaphosphates (IHP), especially the neo-IHP stereoisomer that likely originates from microbes rather than plants, was significantly increased in native grasslands than croplands. Annual croplands had the largest variances of soil P composition, and the highest potential capacity for P cycling processes based on the abundance of genes coding for P cycling processes. In contrast, roadside soils had the highest soil Olsen-P concentrations, lowest organic P, and highest tricalcium phosphate concentrations, which were likely facilitated by the neutral pH and high exchangeable Ca of these soils. Redundancy analysis demonstrated that IHP by NMR, potential phosphatase activity, Olsen-P, and pH were important P chemistry predictors of the P cycling bacterial community and functional gene composition. Combining chemical and metagenomics results provides important insights into soil P processes and dynamics in different land-use ecosystems.https://www.frontiersin.org/article/10.3389/fmicb.2018.01643/fullland usesoilphosphorussolution NMRXANESshotgun metagenomics |
| spellingShingle | Jin Liu Jin Liu Barbara J. Cade-Menun Jianjun Yang Yongfeng Hu Corey W. Liu Julien Tremblay Kerry LaForge Michael Schellenberg Chantal Hamel Luke D. Bainard Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils Frontiers in Microbiology land use soil phosphorus solution NMR XANES shotgun metagenomics |
| title | Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils |
| title_full | Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils |
| title_fullStr | Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils |
| title_full_unstemmed | Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils |
| title_short | Long-Term Land Use Affects Phosphorus Speciation and the Composition of Phosphorus Cycling Genes in Agricultural Soils |
| title_sort | long term land use affects phosphorus speciation and the composition of phosphorus cycling genes in agricultural soils |
| topic | land use soil phosphorus solution NMR XANES shotgun metagenomics |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01643/full |
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