Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer
Growing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients wit...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Molecular & Cellular Oncology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23723556.2023.2188858 |
| _version_ | 1797677105149902848 |
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| author | Dalila Luciola Zanette Karoline Brito Caetano Andrade Coelho Eneas de Carvalho Mateus Nobrega Aoki Jeanine Marie Nardin Larissa Araújo Lalli Rafael dos Santos Bezerra Marta Giovanetti Victória Simionatto Zucherato Gabriel Montenegro de Campos Jardelina de Souza Todão Bernardino Vincent Louis Viala Massimo Ciccozzi Luiz Carlos Junior Alcantara Sandra Coccuzzo Sampaio Maria Carolina Elias Simone Kashima Dimas Tadeu Covas Svetoslav Nanev Slavov |
| author_facet | Dalila Luciola Zanette Karoline Brito Caetano Andrade Coelho Eneas de Carvalho Mateus Nobrega Aoki Jeanine Marie Nardin Larissa Araújo Lalli Rafael dos Santos Bezerra Marta Giovanetti Victória Simionatto Zucherato Gabriel Montenegro de Campos Jardelina de Souza Todão Bernardino Vincent Louis Viala Massimo Ciccozzi Luiz Carlos Junior Alcantara Sandra Coccuzzo Sampaio Maria Carolina Elias Simone Kashima Dimas Tadeu Covas Svetoslav Nanev Slavov |
| author_sort | Dalila Luciola Zanette |
| collection | DOAJ |
| description | Growing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients with prostate cancer. Blood samples were obtained from 49 male patients with primary prostate adenocarcinoma. Thirty blood donors were included as a control group. The obtained next-generation sequencing data were analyzed using a bioinformatic pipeline for virus metagenomics. Viral reads with higher abundance were assembled in contigs and analyzed taxonomically. Viral agents of interest were also confirmed by qPCR. Anelloviruses and the Human Pegivirus-1 (HPgV-1) were the most abundant component of plasma metavirome. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus and human adenovirus type C were also identified. In comparison, the blood donor virome was exclusively composed of torque teno virus types (TTV) types. The performed HPgV-1 and HCV phylogeny revealed that these viruses belong to commonly detected in Brazil genotypes. Our study sheds light on the plasma viral abundance in patients with prostatic cancer. The obtained viral diversity allowed us to separate the patients and controls, probably suggesting that malignant processes may influence virome composition. More complex and multiple approach investigations are necessary to examine the likely causal relationship between metavirome and its nvolvement in prostate cancer. |
| first_indexed | 2024-03-11T22:40:11Z |
| format | Article |
| id | doaj.art-c3da2442e9f441cf8818921de67b43e0 |
| institution | Directory Open Access Journal |
| issn | 2372-3556 |
| language | English |
| last_indexed | 2024-03-11T22:40:11Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Molecular & Cellular Oncology |
| spelling | doaj.art-c3da2442e9f441cf8818921de67b43e02023-09-22T09:19:44ZengTaylor & Francis GroupMolecular & Cellular Oncology2372-35562023-12-0110110.1080/23723556.2023.21888582188858Metagenomic insights into the plasma virome of Brazilian patients with prostate cancerDalila Luciola Zanette0Karoline Brito Caetano Andrade Coelho1Eneas de Carvalho2Mateus Nobrega Aoki3Jeanine Marie Nardin4Larissa Araújo Lalli5Rafael dos Santos Bezerra6Marta Giovanetti7Victória Simionatto Zucherato8Gabriel Montenegro de Campos9Jardelina de Souza Todão Bernardino10Vincent Louis Viala11Massimo Ciccozzi12Luiz Carlos Junior Alcantara13Sandra Coccuzzo Sampaio14Maria Carolina Elias15Simone Kashima16Dimas Tadeu Covas17Svetoslav Nanev Slavov18Oswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz MaderUniversity of São PauloButantan InstituteOswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz MaderErasto Gaertner HospitalOswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz MaderUniversity of Sao PauloUniversity of Campus Bio-Medico di RomaUniversity of Sao PauloUniversity of Sao PauloButantan InstituteButantan InstituteUniversity of Campus Bio-Medico di RomaOswaldo Cruz FoundationButantan InstituteButantan InstituteUniversity of Sao PauloButantan InstituteButantan InstituteGrowing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients with prostate cancer. Blood samples were obtained from 49 male patients with primary prostate adenocarcinoma. Thirty blood donors were included as a control group. The obtained next-generation sequencing data were analyzed using a bioinformatic pipeline for virus metagenomics. Viral reads with higher abundance were assembled in contigs and analyzed taxonomically. Viral agents of interest were also confirmed by qPCR. Anelloviruses and the Human Pegivirus-1 (HPgV-1) were the most abundant component of plasma metavirome. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus and human adenovirus type C were also identified. In comparison, the blood donor virome was exclusively composed of torque teno virus types (TTV) types. The performed HPgV-1 and HCV phylogeny revealed that these viruses belong to commonly detected in Brazil genotypes. Our study sheds light on the plasma viral abundance in patients with prostatic cancer. The obtained viral diversity allowed us to separate the patients and controls, probably suggesting that malignant processes may influence virome composition. More complex and multiple approach investigations are necessary to examine the likely causal relationship between metavirome and its nvolvement in prostate cancer.http://dx.doi.org/10.1080/23723556.2023.2188858viromeprostate cancermetagenomicsviral diversitynext-generation sequencing |
| spellingShingle | Dalila Luciola Zanette Karoline Brito Caetano Andrade Coelho Eneas de Carvalho Mateus Nobrega Aoki Jeanine Marie Nardin Larissa Araújo Lalli Rafael dos Santos Bezerra Marta Giovanetti Victória Simionatto Zucherato Gabriel Montenegro de Campos Jardelina de Souza Todão Bernardino Vincent Louis Viala Massimo Ciccozzi Luiz Carlos Junior Alcantara Sandra Coccuzzo Sampaio Maria Carolina Elias Simone Kashima Dimas Tadeu Covas Svetoslav Nanev Slavov Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer Molecular & Cellular Oncology virome prostate cancer metagenomics viral diversity next-generation sequencing |
| title | Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer |
| title_full | Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer |
| title_fullStr | Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer |
| title_full_unstemmed | Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer |
| title_short | Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer |
| title_sort | metagenomic insights into the plasma virome of brazilian patients with prostate cancer |
| topic | virome prostate cancer metagenomics viral diversity next-generation sequencing |
| url | http://dx.doi.org/10.1080/23723556.2023.2188858 |
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