The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth
Nitrite is still regarded as essentially undesirable in foods. Therefore, it is necessary to keep its concentration under control for food safety. The current study was conducted to evaluate the ability of two reference lactic acid strains to deplete nitrite. Lactobacillus acidophilus ROO52 and Str...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2023-12-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
| Subjects: | |
| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/12139 |
| _version_ | 1797362737953636352 |
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| author | Mahdi. A. Al-Shuwaili Murtadha Abbas S.V. Nadezhdin |
| author_facet | Mahdi. A. Al-Shuwaili Murtadha Abbas S.V. Nadezhdin |
| author_sort | Mahdi. A. Al-Shuwaili |
| collection | DOAJ |
| description |
Nitrite is still regarded as essentially undesirable in foods. Therefore, it is necessary to keep its concentration under control for food safety. The current study was conducted to evaluate the ability of two reference lactic acid strains to deplete nitrite. Lactobacillus acidophilus ROO52 and Streptococcus thermophiles YC-180 were examined in De Man Rogosa and Sharpe (MRS) and M17 broth with standard sodium nitrite at 0.10 mg /mL and 1 mL (10-8 cells per mL) for each of the reference strains to determine the ability of these strains to eliminate nitrite during the fermentation period. A control group, MRS and M17 broth and standard nitrite without inoculum were used. The nitrite depletion was determined by a UV-Vis spectrophotometer. After 48 hours of fermentation, there were significant differences between residual nitrite levels of broth inoculated by Streptococcus thermophiles YC-180 compared to control and Lactobacillus acidophilus ROO52. Streptococcus thermophiles YC-180 depleted nitrite levels significantly (P˂0.05) from the initial concentration (0.10 mg/ml) to 0.02 mg/ml with a percentage (45%) after 48 hours compared to control and Lactobacillus acidophilus ROO52, which depleted nitrite levels from the initial concentration of 0.10 mg/ml to 0.04 mg/ml with a percentage (22.5%) after 48 hours. In conclusion, Streptococcus thermophiles YC-180 was more effective at nitrite depletion than Lactobacillus acidophilus ROO52. The reduction of nitrite occurs due to acid production and the enzymatic action of these bacteria.
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| first_indexed | 2024-03-08T16:11:43Z |
| format | Article |
| id | doaj.art-c3da6b5853ab4a76af673eb38577f4e4 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:11:43Z |
| publishDate | 2023-12-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-c3da6b5853ab4a76af673eb38577f4e42024-01-07T16:34:19ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782023-12-0114210.36326/kjvs/2023/v14i212139The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 BrothMahdi. A. Al-Shuwaili0Murtadha Abbashttps://orcid.org/0000-0002-4486-6290S.V. Nadezhdinhttps://orcid.org/0000-0002-6249-2464Department of Public Health, Faculty of Veterinary Medicine, University of Kufa, Iraq Nitrite is still regarded as essentially undesirable in foods. Therefore, it is necessary to keep its concentration under control for food safety. The current study was conducted to evaluate the ability of two reference lactic acid strains to deplete nitrite. Lactobacillus acidophilus ROO52 and Streptococcus thermophiles YC-180 were examined in De Man Rogosa and Sharpe (MRS) and M17 broth with standard sodium nitrite at 0.10 mg /mL and 1 mL (10-8 cells per mL) for each of the reference strains to determine the ability of these strains to eliminate nitrite during the fermentation period. A control group, MRS and M17 broth and standard nitrite without inoculum were used. The nitrite depletion was determined by a UV-Vis spectrophotometer. After 48 hours of fermentation, there were significant differences between residual nitrite levels of broth inoculated by Streptococcus thermophiles YC-180 compared to control and Lactobacillus acidophilus ROO52. Streptococcus thermophiles YC-180 depleted nitrite levels significantly (P˂0.05) from the initial concentration (0.10 mg/ml) to 0.02 mg/ml with a percentage (45%) after 48 hours compared to control and Lactobacillus acidophilus ROO52, which depleted nitrite levels from the initial concentration of 0.10 mg/ml to 0.04 mg/ml with a percentage (22.5%) after 48 hours. In conclusion, Streptococcus thermophiles YC-180 was more effective at nitrite depletion than Lactobacillus acidophilus ROO52. The reduction of nitrite occurs due to acid production and the enzymatic action of these bacteria. https://journal.uokufa.edu.iq/index.php/kjvs/article/view/12139lactic acid bacterianitritereduction |
| spellingShingle | Mahdi. A. Al-Shuwaili Murtadha Abbas S.V. Nadezhdin The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth Kufa Journal for Veterinary Medical Sciences lactic acid bacteria nitrite reduction |
| title | The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth |
| title_full | The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth |
| title_fullStr | The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth |
| title_full_unstemmed | The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth |
| title_short | The Abilities of Different Lactic Acid Bacterial Stains to Deplete Nitrite Level in MRS and M17 Broth |
| title_sort | abilities of different lactic acid bacterial stains to deplete nitrite level in mrs and m17 broth |
| topic | lactic acid bacteria nitrite reduction |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/12139 |
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