A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize
Abstract Lysine-ε-acetylation (Kac) is a reversible post-translational modification that plays important roles during plant-pathogen interactions. Some pathogens can deliver secreted effectors encoding acetyltransferases or deacetylases into host cell to directly modify acetylation of host proteins....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-12-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03964-4 |
| _version_ | 1797977562924711936 |
|---|---|
| author | Jianfei Guo Zhigang Ma Ce Deng Junqiang Ding Yuxiao Chang |
| author_facet | Jianfei Guo Zhigang Ma Ce Deng Junqiang Ding Yuxiao Chang |
| author_sort | Jianfei Guo |
| collection | DOAJ |
| description | Abstract Lysine-ε-acetylation (Kac) is a reversible post-translational modification that plays important roles during plant-pathogen interactions. Some pathogens can deliver secreted effectors encoding acetyltransferases or deacetylases into host cell to directly modify acetylation of host proteins. However, the function of these acetylated host proteins in plant-pathogen defense remains to be determined. Employing high-resolution tandem mass spectrometry, we analyzed protein abundance and lysine acetylation changes in maize infected with Puccinia polysora (P. polysora) at 0 h, 12 h, 24 h, 48 h and 72 h. A total of 7412 Kac sites from 4697 proteins were identified, and 1732 Kac sites from 1006 proteins were quantified. Analyzed the features of lysine acetylation, we found that Kac is ubiquitous in cellular compartments and preferentially targets lysine residues in the -F/W/Y-X-X-K (ac)-N/S/T/P/Y/G- motif of the protein, this Kac motif contained proteins enriched in basic metabolism and defense-associated pathways during fungal infection. Further analysis of acetylproteomics data indicated that maize regulates cellular processes in response to P. polysora infection by altering Kac levels of histones and non-histones. In addition, acetylation of pathogen defense-related proteins presented converse patterns in signaling transduction, defense response, cell wall fortification, ROS scavenging, redox reaction and proteostasis. Our results provide informative resources for studying protein acetylation in plant-pathogen interactions, not only greatly extending the understanding on the roles of acetylation in vivo, but also providing a comprehensive dynamic pattern of Kac modifications in the process of plant immune response. |
| first_indexed | 2024-04-11T05:08:56Z |
| format | Article |
| id | doaj.art-c3dd3dc9d3534c198fb583bd436985a1 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-11T05:08:56Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-c3dd3dc9d3534c198fb583bd436985a12022-12-25T12:10:08ZengBMCBMC Plant Biology1471-22292022-12-0122112010.1186/s12870-022-03964-4A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maizeJianfei Guo0Zhigang Ma1Ce Deng2Junqiang Ding3Yuxiao Chang4Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityShenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesAbstract Lysine-ε-acetylation (Kac) is a reversible post-translational modification that plays important roles during plant-pathogen interactions. Some pathogens can deliver secreted effectors encoding acetyltransferases or deacetylases into host cell to directly modify acetylation of host proteins. However, the function of these acetylated host proteins in plant-pathogen defense remains to be determined. Employing high-resolution tandem mass spectrometry, we analyzed protein abundance and lysine acetylation changes in maize infected with Puccinia polysora (P. polysora) at 0 h, 12 h, 24 h, 48 h and 72 h. A total of 7412 Kac sites from 4697 proteins were identified, and 1732 Kac sites from 1006 proteins were quantified. Analyzed the features of lysine acetylation, we found that Kac is ubiquitous in cellular compartments and preferentially targets lysine residues in the -F/W/Y-X-X-K (ac)-N/S/T/P/Y/G- motif of the protein, this Kac motif contained proteins enriched in basic metabolism and defense-associated pathways during fungal infection. Further analysis of acetylproteomics data indicated that maize regulates cellular processes in response to P. polysora infection by altering Kac levels of histones and non-histones. In addition, acetylation of pathogen defense-related proteins presented converse patterns in signaling transduction, defense response, cell wall fortification, ROS scavenging, redox reaction and proteostasis. Our results provide informative resources for studying protein acetylation in plant-pathogen interactions, not only greatly extending the understanding on the roles of acetylation in vivo, but also providing a comprehensive dynamic pattern of Kac modifications in the process of plant immune response.https://doi.org/10.1186/s12870-022-03964-4Southern corn rust (SCR)Mass spectrometryQuantitative proteomicsLysine acetylationPlant-pathogen interactionImmune response |
| spellingShingle | Jianfei Guo Zhigang Ma Ce Deng Junqiang Ding Yuxiao Chang A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize BMC Plant Biology Southern corn rust (SCR) Mass spectrometry Quantitative proteomics Lysine acetylation Plant-pathogen interaction Immune response |
| title | A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize |
| title_full | A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize |
| title_fullStr | A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize |
| title_full_unstemmed | A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize |
| title_short | A comprehensive dynamic immune acetylproteomics profiling induced by Puccinia polysora in maize |
| title_sort | comprehensive dynamic immune acetylproteomics profiling induced by puccinia polysora in maize |
| topic | Southern corn rust (SCR) Mass spectrometry Quantitative proteomics Lysine acetylation Plant-pathogen interaction Immune response |
| url | https://doi.org/10.1186/s12870-022-03964-4 |
| work_keys_str_mv | AT jianfeiguo acomprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT zhigangma acomprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT cedeng acomprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT junqiangding acomprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT yuxiaochang acomprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT jianfeiguo comprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT zhigangma comprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT cedeng comprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT junqiangding comprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize AT yuxiaochang comprehensivedynamicimmuneacetylproteomicsprofilinginducedbypucciniapolysorainmaize |