Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis
Conyza canadensis has been reported to be the most frequent weed species that evolved resistance to glyphosate in various parts of the world. The objective of the present study was to investigate the effect of environmental conditions (temperature and light) on the expression levels of the EPSPS gen...
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| Language: | English |
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MDPI AG
2016-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/17/4/342 |
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| author | Eleni Tani Demosthenis Chachalis Ilias S. Travlos Dimitrios Bilalis |
| author_facet | Eleni Tani Demosthenis Chachalis Ilias S. Travlos Dimitrios Bilalis |
| author_sort | Eleni Tani |
| collection | DOAJ |
| description | Conyza canadensis has been reported to be the most frequent weed species that evolved resistance to glyphosate in various parts of the world. The objective of the present study was to investigate the effect of environmental conditions (temperature and light) on the expression levels of the EPSPS gene and two major ABC-transporter genes (M10 and M11) on glyphosate susceptible (GS) and glyphosate resistant (GR) horseweed populations, collected from several regions across Greece. Real-time PCR was conducted to determine the expression level of the aforementioned genes when glyphosate was applied at normal (1×; 533 g·a.e.·ha−1) and high rates (4×, 8×), measured at an early one day after treatment (DAT) and a later stage (four DAT) of expression. Plants were exposed to light or dark conditions, at three temperature regimes (8, 25, 35 °C). GR plants were made sensitive when exposed to 8 °C with light; those sensitized plants behaved biochemically (shikimate accumulation) and molecularly (expression of EPSPS and ABC-genes) like the GS plants. Results from the current study show the direct link between the environmental conditions and the induction level of the above key genes that likely affect the efficiency of the proposed mechanism of glyphosate resistance. |
| first_indexed | 2024-04-12T14:41:56Z |
| format | Article |
| id | doaj.art-c3e149b722c74594b7adf0525e27b8ab |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T14:41:56Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c3e149b722c74594b7adf0525e27b8ab2022-12-22T03:28:49ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-04-0117434210.3390/ijms17040342ijms17040342Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensisEleni Tani0Demosthenis Chachalis1Ilias S. Travlos2Dimitrios Bilalis3Laboratory of Plant Breeding, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceLaboratory of Weed Science, Benaki Phytopathological Institute, S. Delta 8, 14561 Athens, GreeceLaboratory of Agronomy, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceLaboratory of Agronomy, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceConyza canadensis has been reported to be the most frequent weed species that evolved resistance to glyphosate in various parts of the world. The objective of the present study was to investigate the effect of environmental conditions (temperature and light) on the expression levels of the EPSPS gene and two major ABC-transporter genes (M10 and M11) on glyphosate susceptible (GS) and glyphosate resistant (GR) horseweed populations, collected from several regions across Greece. Real-time PCR was conducted to determine the expression level of the aforementioned genes when glyphosate was applied at normal (1×; 533 g·a.e.·ha−1) and high rates (4×, 8×), measured at an early one day after treatment (DAT) and a later stage (four DAT) of expression. Plants were exposed to light or dark conditions, at three temperature regimes (8, 25, 35 °C). GR plants were made sensitive when exposed to 8 °C with light; those sensitized plants behaved biochemically (shikimate accumulation) and molecularly (expression of EPSPS and ABC-genes) like the GS plants. Results from the current study show the direct link between the environmental conditions and the induction level of the above key genes that likely affect the efficiency of the proposed mechanism of glyphosate resistance.http://www.mdpi.com/1422-0067/17/4/342Conyza canadensisglyphosate resistanceABC-transporter genesEPSPSenvironmental factors |
| spellingShingle | Eleni Tani Demosthenis Chachalis Ilias S. Travlos Dimitrios Bilalis Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis International Journal of Molecular Sciences Conyza canadensis glyphosate resistance ABC-transporter genes EPSPS environmental factors |
| title | Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis |
| title_full | Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis |
| title_fullStr | Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis |
| title_full_unstemmed | Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis |
| title_short | Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis |
| title_sort | environmental conditions influence induction of key abc transporter genes affecting glyphosate resistance mechanism in conyza canadensis |
| topic | Conyza canadensis glyphosate resistance ABC-transporter genes EPSPS environmental factors |
| url | http://www.mdpi.com/1422-0067/17/4/342 |
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