The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model
Objective: The aim of the present study was to evaluate the effects of PRP on Achilles tendon healing in rabbits during the inflammatory, proliferative, and remodeling phases by histological examination and quantitative assessments. Methods: Fifty mature male Japanese albino rabbits with severed Ach...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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AVES
2017-01-01
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| Series: | Acta Orthopaedica et Traumatologica Turcica |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1017995X16303509 |
| _version_ | 1828028420627365888 |
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| author | Masaki Takamura Toshito Yasuda Atsushi Nakano Hiroaki Shima Masashi Neo |
| author_facet | Masaki Takamura Toshito Yasuda Atsushi Nakano Hiroaki Shima Masashi Neo |
| author_sort | Masaki Takamura |
| collection | DOAJ |
| description | Objective: The aim of the present study was to evaluate the effects of PRP on Achilles tendon healing in rabbits during the inflammatory, proliferative, and remodeling phases by histological examination and quantitative assessments. Methods: Fifty mature male Japanese albino rabbits with severed Achilles tendons were divided into two equal groups and treated with platelet-rich plasma (PRP) or left untreated. Tendon tissue was harvested at 1, 2, 3, 4, and 6 weeks after treatment, and sections were stained with hematoxylin-eosin and monoclonal antibodies against CD31 and type I collagen. Results: Collagen fibers proliferated more densely early after severance, and subsequent remodeling of the collagen fibers and approximation of normal tendinous tissue occurred earlier in the PRP group than in the control group. The fibroblast number was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Similarly, the area ratio of CD31-positive cells was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Positive staining for type I collagen was more intense in the PRP group than in the control group after 3 weeks, indicating tendon maturation. Conclusion: Administration of PRP shortened the inflammatory phase and promoted tendon healing during the proliferative phase. Keywords: Achilles tendon, Growth factor, Platelet-rich plasma, Rabbit, Tendon healing |
| first_indexed | 2024-04-10T13:52:27Z |
| format | Article |
| id | doaj.art-c3e1995f85bc408fbce2df3411c84024 |
| institution | Directory Open Access Journal |
| issn | 1017-995X |
| language | English |
| last_indexed | 2024-04-10T13:52:27Z |
| publishDate | 2017-01-01 |
| publisher | AVES |
| record_format | Article |
| series | Acta Orthopaedica et Traumatologica Turcica |
| spelling | doaj.art-c3e1995f85bc408fbce2df3411c840242023-02-15T16:10:38ZengAVESActa Orthopaedica et Traumatologica Turcica1017-995X2017-01-015116572The effect of platelet-rich plasma on Achilles tendon healing in a rabbit modelMasaki Takamura0Toshito Yasuda1Atsushi Nakano2Hiroaki Shima3Masashi Neo4Corresponding author. Department of Orthopedic Surgery, Osaka Medical College, 2-7 Daigaku-Machi, Takatsuki, Osaka 569-8686, Japan. Fax: +81 726 83 6265.; Department of Orthopedic Surgery, Osaka Medical College, Osaka, JapanDepartment of Orthopedic Surgery, Osaka Medical College, Osaka, JapanDepartment of Orthopedic Surgery, Osaka Medical College, Osaka, JapanDepartment of Orthopedic Surgery, Osaka Medical College, Osaka, JapanDepartment of Orthopedic Surgery, Osaka Medical College, Osaka, JapanObjective: The aim of the present study was to evaluate the effects of PRP on Achilles tendon healing in rabbits during the inflammatory, proliferative, and remodeling phases by histological examination and quantitative assessments. Methods: Fifty mature male Japanese albino rabbits with severed Achilles tendons were divided into two equal groups and treated with platelet-rich plasma (PRP) or left untreated. Tendon tissue was harvested at 1, 2, 3, 4, and 6 weeks after treatment, and sections were stained with hematoxylin-eosin and monoclonal antibodies against CD31 and type I collagen. Results: Collagen fibers proliferated more densely early after severance, and subsequent remodeling of the collagen fibers and approximation of normal tendinous tissue occurred earlier in the PRP group than in the control group. The fibroblast number was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Similarly, the area ratio of CD31-positive cells was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Positive staining for type I collagen was more intense in the PRP group than in the control group after 3 weeks, indicating tendon maturation. Conclusion: Administration of PRP shortened the inflammatory phase and promoted tendon healing during the proliferative phase. Keywords: Achilles tendon, Growth factor, Platelet-rich plasma, Rabbit, Tendon healinghttp://www.sciencedirect.com/science/article/pii/S1017995X16303509 |
| spellingShingle | Masaki Takamura Toshito Yasuda Atsushi Nakano Hiroaki Shima Masashi Neo The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model Acta Orthopaedica et Traumatologica Turcica |
| title | The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model |
| title_full | The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model |
| title_fullStr | The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model |
| title_full_unstemmed | The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model |
| title_short | The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model |
| title_sort | effect of platelet rich plasma on achilles tendon healing in a rabbit model |
| url | http://www.sciencedirect.com/science/article/pii/S1017995X16303509 |
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