Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria
Plasmid mediated quinolone resistance (PMQR) is a public health challenge arising among other things, from indiscriminate use of the floroquinolones (FQr) prophylactically in animal husbandry. This study examines the occurrence of PMQR genes amongst enteric bacteria isolated from human and animal so...
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AIMS Press
2021-02-01
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| Series: | AIMS Microbiology |
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| Online Access: | http://www.aimspress.com/article/doi/10.3934/microbiol.2021006?viewType=HTML |
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| author | EHWARIEME Daniel Ayobola WHILIKI Onoriadjeren Oscar EJUKONEMU Francis Ejovwokoghene |
| author_facet | EHWARIEME Daniel Ayobola WHILIKI Onoriadjeren Oscar EJUKONEMU Francis Ejovwokoghene |
| author_sort | EHWARIEME Daniel Ayobola |
| collection | DOAJ |
| description | Plasmid mediated quinolone resistance (PMQR) is a public health challenge arising among other things, from indiscriminate use of the floroquinolones (FQr) prophylactically in animal husbandry. This study examines the occurrence of PMQR genes amongst enteric bacteria isolated from human and animal sources. A total of 720 (360 stool and 360 fish pond water/poultry litter) samples were examined for fluoroquinolone resistant (FQr) bacteria. Percentage FQr was generally higher among human isolates than isolates from animals. Proportion of PMQR amongst FQr isolates were (1.05 and 4.32) % for <i>E</i>. <i>coli</i> from human and animal sources. For <i>Salmonella</i> spp., <i>Shigella</i> spp., <i>Klebsiella</i> spp. and <i>Aeromonas</i> spp., percentages PMQR were 0.00 & 6.93, 0.00 & 6.38, 4.26 & 5.26 and 0.00 &3.03 for human and animal sources respectively, for the isolates. The PMQR genes: <i>qnr</i>A, <i>qnr</i> B, <i>qnr</i> S and <i>qep</i> A were 11, 15, 7 and 1 amongst a total of 1018 FQr and 29 PMQR isolates respectively. The aac (6′)–Ib-cr gene was not detected in this study. Approximate Plasmid bands of PCR amplicon for <i>qnr</i> A, <i>qnr</i> B, <i>qnr</i> S and <i>qep</i> A respectively were established. The proportion of PMQR genes especially among isolates from animal sources is of public health concern due to the higher possibility of a horizontal FQ resistance transfer to humans. |
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| id | doaj.art-c3e205f878ee4e60b627409dcaf3cde0 |
| institution | Directory Open Access Journal |
| issn | 2471-1888 |
| language | English |
| last_indexed | 2024-12-20T08:15:57Z |
| publishDate | 2021-02-01 |
| publisher | AIMS Press |
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| series | AIMS Microbiology |
| spelling | doaj.art-c3e205f878ee4e60b627409dcaf3cde02022-12-21T19:47:07ZengAIMS PressAIMS Microbiology2471-18882021-02-0171759510.3934/microbiol.2021006Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, NigeriaEHWARIEME Daniel Ayobola0WHILIKI Onoriadjeren Oscar1EJUKONEMU Francis Ejovwokoghene2. Department of Microbiology, Faculty of Science, Delta State University, Abraka, Nigeria2. Department of Science Laboratory Technology, Delta State Polytechnic, Otefe-Oghara, Nigeria2. Department of Science Laboratory Technology, Delta State Polytechnic, Otefe-Oghara, NigeriaPlasmid mediated quinolone resistance (PMQR) is a public health challenge arising among other things, from indiscriminate use of the floroquinolones (FQr) prophylactically in animal husbandry. This study examines the occurrence of PMQR genes amongst enteric bacteria isolated from human and animal sources. A total of 720 (360 stool and 360 fish pond water/poultry litter) samples were examined for fluoroquinolone resistant (FQr) bacteria. Percentage FQr was generally higher among human isolates than isolates from animals. Proportion of PMQR amongst FQr isolates were (1.05 and 4.32) % for <i>E</i>. <i>coli</i> from human and animal sources. For <i>Salmonella</i> spp., <i>Shigella</i> spp., <i>Klebsiella</i> spp. and <i>Aeromonas</i> spp., percentages PMQR were 0.00 & 6.93, 0.00 & 6.38, 4.26 & 5.26 and 0.00 &3.03 for human and animal sources respectively, for the isolates. The PMQR genes: <i>qnr</i>A, <i>qnr</i> B, <i>qnr</i> S and <i>qep</i> A were 11, 15, 7 and 1 amongst a total of 1018 FQr and 29 PMQR isolates respectively. The aac (6′)–Ib-cr gene was not detected in this study. Approximate Plasmid bands of PCR amplicon for <i>qnr</i> A, <i>qnr</i> B, <i>qnr</i> S and <i>qep</i> A respectively were established. The proportion of PMQR genes especially among isolates from animal sources is of public health concern due to the higher possibility of a horizontal FQ resistance transfer to humans.http://www.aimspress.com/article/doi/10.3934/microbiol.2021006?viewType=HTMLfluoroquinolonesenterobacteriaceaeanimal husbandry |
| spellingShingle | EHWARIEME Daniel Ayobola WHILIKI Onoriadjeren Oscar EJUKONEMU Francis Ejovwokoghene Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria AIMS Microbiology fluoroquinolones enterobacteriaceae animal husbandry |
| title | Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria |
| title_full | Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria |
| title_fullStr | Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria |
| title_full_unstemmed | Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria |
| title_short | Occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in Delta State, Nigeria |
| title_sort | occurrence of plasmid mediated fluoroquinolone resistance genes amongst enteric bacteria isolated from human and animal sources in delta state nigeria |
| topic | fluoroquinolones enterobacteriaceae animal husbandry |
| url | http://www.aimspress.com/article/doi/10.3934/microbiol.2021006?viewType=HTML |
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