Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes
Flooding is a frequent environmental stress that reduces soybean (<i>Glycine max</i>) growth and grain yield in many producing areas in the world, such as, e.g., in the United States, Southeast Asia and Southern Brazil. In these regions, soybean is frequently cultivated in lowland areas...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10611 |
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| author | Luisa Abruzzi de Oliveira-Busatto Cecilia Paz da Silva Giordano Marília Ferreira da Silva Darci Uhry Junior Frank Guzman Beatriz Wiebke-Strohm Zenglu Li Christian Bredemeier Maria Helena Bodanese-Zanettini |
| author_facet | Luisa Abruzzi de Oliveira-Busatto Cecilia Paz da Silva Giordano Marília Ferreira da Silva Darci Uhry Junior Frank Guzman Beatriz Wiebke-Strohm Zenglu Li Christian Bredemeier Maria Helena Bodanese-Zanettini |
| author_sort | Luisa Abruzzi de Oliveira-Busatto |
| collection | DOAJ |
| description | Flooding is a frequent environmental stress that reduces soybean (<i>Glycine max</i>) growth and grain yield in many producing areas in the world, such as, e.g., in the United States, Southeast Asia and Southern Brazil. In these regions, soybean is frequently cultivated in lowland areas by rotating with rice (<i>Oryza sativa</i>), which provides numerous technical, economic and environmental benefits. Given these realities, this work aimed to characterize physiological responses, identify genes differentially expressed under flooding stress in Brazilian soybean genotypes with contrasting flooding tolerance, and select SNPs with potential use for marker-assisted selection. Soybean cultivars TECIRGA 6070 (flooding tolerant) and FUNDACEP 62 (flooding sensitive) were grown up to the V6 growth stage and then flooding stress was imposed. Total RNA was extracted from leaves 24 h after the stress was imposed and sequenced. In total, 421 induced and 291 repressed genes were identified in both genotypes. TECIRGA 6070 presented 284 and 460 genes up- and down-regulated, respectively, under flooding conditions. Of those, 100 and 148 genes were exclusively up- and down-regulated, respectively, in the tolerant genotype. Based on the RNA sequencing data, SNPs in differentially expressed genes in response to flooding stress were identified. Finally, 38 SNPs, located in genes with functional annotation for response to abiotic stresses, were found in TECIRGA 6070 and absent in FUNDACEP 62. To validate them, 22 SNPs were selected for designing KASP assays that were used to genotype a panel of 11 contrasting genotypes with known phenotypes. In addition, the phenotypic and grain yield impacts were analyzed in four field experiments using a panel of 166 Brazilian soybean genotypes. Five SNPs possibly related to flooding tolerance in Brazilian soybean genotypes were identified. The information generated from this research will be useful to develop soybean genotypes adapted to poorly drained soils or areas subject to flooding. |
| first_indexed | 2024-03-09T23:45:08Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:45:08Z |
| publishDate | 2022-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c3e230f4f21041bca9e576468ff646b52023-11-23T16:45:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181061110.3390/ijms231810611Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean GenotypesLuisa Abruzzi de Oliveira-Busatto0Cecilia Paz da Silva Giordano1Marília Ferreira da Silva2Darci Uhry Junior3Frank Guzman4Beatriz Wiebke-Strohm5Zenglu Li6Christian Bredemeier7Maria Helena Bodanese-Zanettini8Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia: Biotec Seca-Pragas, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BrazilPrograma de Pós-Graduação em Fitotecnia, Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, BrazilPrograma de Pós-Graduação em Fitotecnia, Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, BrazilPrograma de Pós-Graduação em Fitotecnia, Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, BrazilEscuela de Medicina, Universidad Científica del Sur, Lima 15067, PeruPrograma de Pós-Graduação em Genética e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia: Biotec Seca-Pragas, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BrazilDepartment of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USAPrograma de Pós-Graduação em Fitotecnia, Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, BrazilPrograma de Pós-Graduação em Genética e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia: Biotec Seca-Pragas, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BrazilFlooding is a frequent environmental stress that reduces soybean (<i>Glycine max</i>) growth and grain yield in many producing areas in the world, such as, e.g., in the United States, Southeast Asia and Southern Brazil. In these regions, soybean is frequently cultivated in lowland areas by rotating with rice (<i>Oryza sativa</i>), which provides numerous technical, economic and environmental benefits. Given these realities, this work aimed to characterize physiological responses, identify genes differentially expressed under flooding stress in Brazilian soybean genotypes with contrasting flooding tolerance, and select SNPs with potential use for marker-assisted selection. Soybean cultivars TECIRGA 6070 (flooding tolerant) and FUNDACEP 62 (flooding sensitive) were grown up to the V6 growth stage and then flooding stress was imposed. Total RNA was extracted from leaves 24 h after the stress was imposed and sequenced. In total, 421 induced and 291 repressed genes were identified in both genotypes. TECIRGA 6070 presented 284 and 460 genes up- and down-regulated, respectively, under flooding conditions. Of those, 100 and 148 genes were exclusively up- and down-regulated, respectively, in the tolerant genotype. Based on the RNA sequencing data, SNPs in differentially expressed genes in response to flooding stress were identified. Finally, 38 SNPs, located in genes with functional annotation for response to abiotic stresses, were found in TECIRGA 6070 and absent in FUNDACEP 62. To validate them, 22 SNPs were selected for designing KASP assays that were used to genotype a panel of 11 contrasting genotypes with known phenotypes. In addition, the phenotypic and grain yield impacts were analyzed in four field experiments using a panel of 166 Brazilian soybean genotypes. Five SNPs possibly related to flooding tolerance in Brazilian soybean genotypes were identified. The information generated from this research will be useful to develop soybean genotypes adapted to poorly drained soils or areas subject to flooding.https://www.mdpi.com/1422-0067/23/18/10611molecular markersRNA-SeqSNPsabiotic stressphenotyping |
| spellingShingle | Luisa Abruzzi de Oliveira-Busatto Cecilia Paz da Silva Giordano Marília Ferreira da Silva Darci Uhry Junior Frank Guzman Beatriz Wiebke-Strohm Zenglu Li Christian Bredemeier Maria Helena Bodanese-Zanettini Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes International Journal of Molecular Sciences molecular markers RNA-Seq SNPs abiotic stress phenotyping |
| title | Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes |
| title_full | Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes |
| title_fullStr | Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes |
| title_full_unstemmed | Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes |
| title_short | Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes |
| title_sort | identification of functional genetic variations underlying flooding tolerance in brazilian soybean genotypes |
| topic | molecular markers RNA-Seq SNPs abiotic stress phenotyping |
| url | https://www.mdpi.com/1422-0067/23/18/10611 |
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