Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic

Abstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivale...

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Main Authors: Thandiwe Alide, Phanice Wangila, Ambrose Kiprop
Format: Article
Language:English
Published: BMC 2020-12-01
Series:BMC Research Notes
Subjects:
Online Access:https://doi.org/10.1186/s13104-020-05404-8
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author Thandiwe Alide
Phanice Wangila
Ambrose Kiprop
author_facet Thandiwe Alide
Phanice Wangila
Ambrose Kiprop
author_sort Thandiwe Alide
collection DOAJ
description Abstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivalent per 100 g (GAE/100 g) and 638.96 ± 15.30 mg GAE/100 g of plant material for the aqueous and ethanolic extracts respectively. The mean total flavonoid content 109.78 ± 6.78 mg quercetin equivalent per 100 g (QE/100 g) and 258.47 ± 12.37 QE/100 g for aqueous and ethanolic extracts respectively. Fourier transform infrared spectral data showed absorptions in the range for carboxylic acids, hydroxyl group, esters, and alcohols, confirming the presence of phenols and flavonoids in the extracts. Cooking temperature had a significant effect on total phenolic content and total flavonoid content while cooking time did not have a significant effect on the phytochemicals and antioxidant activity.
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spelling doaj.art-c3e7af666f214276af68869509969c752022-12-21T18:53:33ZengBMCBMC Research Notes1756-05002020-12-011311710.1186/s13104-020-05404-8Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlicThandiwe Alide0Phanice Wangila1Ambrose Kiprop2Department of Chemistry and Biochemistry, School of Sciences and Aerospace Studies, Moi UniversityDepartment of Chemistry and Biochemistry, School of Sciences and Aerospace Studies, Moi UniversityDepartment of Chemistry and Biochemistry, School of Sciences and Aerospace Studies, Moi UniversityAbstract Objective To investigate the effect of cooking temperature and time on the total phenolic content, total flavonoid content and antioxidant activity of aqueous and ethanolic extracts of garlic. Results The mean total phenolic content of fresh garlic were 303.07 ± 6.58 mg gallic acid equivalent per 100 g (GAE/100 g) and 638.96 ± 15.30 mg GAE/100 g of plant material for the aqueous and ethanolic extracts respectively. The mean total flavonoid content 109.78 ± 6.78 mg quercetin equivalent per 100 g (QE/100 g) and 258.47 ± 12.37 QE/100 g for aqueous and ethanolic extracts respectively. Fourier transform infrared spectral data showed absorptions in the range for carboxylic acids, hydroxyl group, esters, and alcohols, confirming the presence of phenols and flavonoids in the extracts. Cooking temperature had a significant effect on total phenolic content and total flavonoid content while cooking time did not have a significant effect on the phytochemicals and antioxidant activity.https://doi.org/10.1186/s13104-020-05404-8Allium sativumFourier transform infrared spectroscopyCondimentAllicinRadical scavenging activity
spellingShingle Thandiwe Alide
Phanice Wangila
Ambrose Kiprop
Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
BMC Research Notes
Allium sativum
Fourier transform infrared spectroscopy
Condiment
Allicin
Radical scavenging activity
title Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
title_full Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
title_fullStr Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
title_full_unstemmed Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
title_short Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic
title_sort effect of cooking temperature and time on total phenolic content total flavonoid content and total in vitro antioxidant activity of garlic
topic Allium sativum
Fourier transform infrared spectroscopy
Condiment
Allicin
Radical scavenging activity
url https://doi.org/10.1186/s13104-020-05404-8
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AT ambrosekiprop effectofcookingtemperatureandtimeontotalphenoliccontenttotalflavonoidcontentandtotalinvitroantioxidantactivityofgarlic