Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product
<i>Salmonella</i> spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use of antimicrobials. We aimed to determine the effects of the food supplement, fermented defatted ‘alperujo’ (FDA), a mod...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1931 |
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| author | Agustín Rebollada-Merino María Ugarte-Ruiz Marta Hernández Pedro Miguela-Villoldo David Abad David Rodríguez-Lázaro Lucía de Juan Lucas Domínguez Antonio Rodríguez-Bertos |
| author_facet | Agustín Rebollada-Merino María Ugarte-Ruiz Marta Hernández Pedro Miguela-Villoldo David Abad David Rodríguez-Lázaro Lucía de Juan Lucas Domínguez Antonio Rodríguez-Bertos |
| author_sort | Agustín Rebollada-Merino |
| collection | DOAJ |
| description | <i>Salmonella</i> spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use of antimicrobials. We aimed to determine the effects of the food supplement, fermented defatted ‘alperujo’ (FDA), a modified olive oil by-product, on <i>Salmonella</i> Typhimurium colonisation in broilers. One hundred and twenty 1-day-old broilers were divided into four experimental groups—two control groups and two treated groups, and challenged with <i>S</i>. Typhimurium at day 7 or 21. On days 7, 14, 21, 28, 35, and 42 of life, duodenum and cecum tissue samples were collected for histopathological and histomorphometric studies. Additionally, cecum content was collected for <i>Salmonella</i> spp. detection by culture and qPCR, and for metagenomic analysis. Our results showed a significant reduction of <i>Salmonella</i> spp. in the cecum of 42-day-old broilers, suggesting that fermented defatted ‘alperujo’ limits <i>Salmonella</i> Typhimurium colonization in that cecum and may contribute to diminishing the risk of carcass contamination at the time of slaughter. The improvement of the mucosal integrity, observed histologically and morphometrically, may contribute to enhancing intestinal health and to limiting <i>Salmonella</i> spp. colonisation in the host, mitigating production losses. These results could provide evidence that FDA would contribute to prophylactic and therapeutic measures to reduce salmonellosis prevalence in poultry farms. |
| first_indexed | 2024-03-10T15:27:53Z |
| format | Article |
| id | doaj.art-c3eceffa9d584803a340a797448d5944 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T15:27:53Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c3eceffa9d584803a340a797448d59442023-11-20T17:54:28ZengMDPI AGAnimals2076-26152020-10-011010193110.3390/ani10101931Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-ProductAgustín Rebollada-Merino0María Ugarte-Ruiz1Marta Hernández2Pedro Miguela-Villoldo3David Abad4David Rodríguez-Lázaro5Lucía de Juan6Lucas Domínguez7Antonio Rodríguez-Bertos8VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, 47071 Valladolid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, 47071 Valladolid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, 47071 Valladolid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain<i>Salmonella</i> spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use of antimicrobials. We aimed to determine the effects of the food supplement, fermented defatted ‘alperujo’ (FDA), a modified olive oil by-product, on <i>Salmonella</i> Typhimurium colonisation in broilers. One hundred and twenty 1-day-old broilers were divided into four experimental groups—two control groups and two treated groups, and challenged with <i>S</i>. Typhimurium at day 7 or 21. On days 7, 14, 21, 28, 35, and 42 of life, duodenum and cecum tissue samples were collected for histopathological and histomorphometric studies. Additionally, cecum content was collected for <i>Salmonella</i> spp. detection by culture and qPCR, and for metagenomic analysis. Our results showed a significant reduction of <i>Salmonella</i> spp. in the cecum of 42-day-old broilers, suggesting that fermented defatted ‘alperujo’ limits <i>Salmonella</i> Typhimurium colonization in that cecum and may contribute to diminishing the risk of carcass contamination at the time of slaughter. The improvement of the mucosal integrity, observed histologically and morphometrically, may contribute to enhancing intestinal health and to limiting <i>Salmonella</i> spp. colonisation in the host, mitigating production losses. These results could provide evidence that FDA would contribute to prophylactic and therapeutic measures to reduce salmonellosis prevalence in poultry farms.https://www.mdpi.com/2076-2615/10/10/1931antimicrobial alternativesfermented defatted ‘alperujo’intestinal healtholive oil by-products<i>Salmonella</i> Typhimurium |
| spellingShingle | Agustín Rebollada-Merino María Ugarte-Ruiz Marta Hernández Pedro Miguela-Villoldo David Abad David Rodríguez-Lázaro Lucía de Juan Lucas Domínguez Antonio Rodríguez-Bertos Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product Animals antimicrobial alternatives fermented defatted ‘alperujo’ intestinal health olive oil by-products <i>Salmonella</i> Typhimurium |
| title | Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product |
| title_full | Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product |
| title_fullStr | Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product |
| title_full_unstemmed | Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product |
| title_short | Reduction of <i>Salmonella</i> Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product |
| title_sort | reduction of i salmonella i typhimurium cecal colonisation and improvement of intestinal health in broilers supplemented with fermented defatted alperujo an olive oil by product |
| topic | antimicrobial alternatives fermented defatted ‘alperujo’ intestinal health olive oil by-products <i>Salmonella</i> Typhimurium |
| url | https://www.mdpi.com/2076-2615/10/10/1931 |
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