Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd
Abstract Background The basic leucine zipper (bZIP) transcription factor family is one of the most abundant and evolutionarily conserved gene families in plants. It assumes crucial functions in the life cycle of plants, including pathogen defense, secondary metabolism, stress response, seed maturati...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04580-6 |
| _version_ | 1797415643272708096 |
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| author | Wei Liu Min Wang Min Zhong Chen Luo Shaoqi Shi Yulei Qian Yunyan Kang Biao Jiang |
| author_facet | Wei Liu Min Wang Min Zhong Chen Luo Shaoqi Shi Yulei Qian Yunyan Kang Biao Jiang |
| author_sort | Wei Liu |
| collection | DOAJ |
| description | Abstract Background The basic leucine zipper (bZIP) transcription factor family is one of the most abundant and evolutionarily conserved gene families in plants. It assumes crucial functions in the life cycle of plants, including pathogen defense, secondary metabolism, stress response, seed maturation, and flower development. Although the genome of wax gourd has been published, little is known about the functions, evolutionary background, and gene expression patterns of the bZIP gene family, which limits its utilization. Results A total of 61 bZIP genes (BhbZIPs) were identified from wax gourd (Benincasa hispida) genome and divided into 12 subgroups. Whole-genome duplication (WGD) and dispersed duplication (DSD) were the main driving forces of bZIP gene family expansion in wax gourd, and this family may have undergone intense purifying selection pressure during the evolutionary process. We selected BhbZIP58, only one in the member of subgroup B, to study its expression patterns under different stresses, including heat, salt, drought, cold stress, and ABA treatment. Surprisingly, BhbZIP58 had a dramatic response under heat stress. BhbZIP58 showed the highest expression level in the root compared with leaves, stem, stamen, pistil, and ovary. In addition, BhbZIP58 protein was located in the nucleus and had transcriptional activation activity. Overexpression of BhbZIP58 in Arabidopsis enhanced their heat tolerance. Conclusions In this study, bZIP gene family is systematically bioinformatically in wax gourd for the first time. Particularly, BhbZIP58 may have an important role in heat stress. It will facilitate further research on the bZIP gene family regarding their evolutionary history and biological functions. |
| first_indexed | 2024-03-09T05:51:34Z |
| format | Article |
| id | doaj.art-c3ef31a5dcf947a5b22d1e5bcfc2b4f8 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T05:51:34Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-c3ef31a5dcf947a5b22d1e5bcfc2b4f82023-12-03T12:17:12ZengBMCBMC Plant Biology1471-22292023-11-0123111610.1186/s12870-023-04580-6Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourdWei Liu0Min Wang1Min Zhong2Chen Luo3Shaoqi Shi4Yulei Qian5Yunyan Kang6Biao Jiang7Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesCollege of Horticulture, South China Agricultural UniversityGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesCollege of Horticulture, South China Agricultural UniversityGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural SciencesAbstract Background The basic leucine zipper (bZIP) transcription factor family is one of the most abundant and evolutionarily conserved gene families in plants. It assumes crucial functions in the life cycle of plants, including pathogen defense, secondary metabolism, stress response, seed maturation, and flower development. Although the genome of wax gourd has been published, little is known about the functions, evolutionary background, and gene expression patterns of the bZIP gene family, which limits its utilization. Results A total of 61 bZIP genes (BhbZIPs) were identified from wax gourd (Benincasa hispida) genome and divided into 12 subgroups. Whole-genome duplication (WGD) and dispersed duplication (DSD) were the main driving forces of bZIP gene family expansion in wax gourd, and this family may have undergone intense purifying selection pressure during the evolutionary process. We selected BhbZIP58, only one in the member of subgroup B, to study its expression patterns under different stresses, including heat, salt, drought, cold stress, and ABA treatment. Surprisingly, BhbZIP58 had a dramatic response under heat stress. BhbZIP58 showed the highest expression level in the root compared with leaves, stem, stamen, pistil, and ovary. In addition, BhbZIP58 protein was located in the nucleus and had transcriptional activation activity. Overexpression of BhbZIP58 in Arabidopsis enhanced their heat tolerance. Conclusions In this study, bZIP gene family is systematically bioinformatically in wax gourd for the first time. Particularly, BhbZIP58 may have an important role in heat stress. It will facilitate further research on the bZIP gene family regarding their evolutionary history and biological functions.https://doi.org/10.1186/s12870-023-04580-6Wax gourdHeat stressGenome-wide identificationEvolutionary patternBhbZIP58 |
| spellingShingle | Wei Liu Min Wang Min Zhong Chen Luo Shaoqi Shi Yulei Qian Yunyan Kang Biao Jiang Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd BMC Plant Biology Wax gourd Heat stress Genome-wide identification Evolutionary pattern BhbZIP58 |
| title | Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd |
| title_full | Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd |
| title_fullStr | Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd |
| title_full_unstemmed | Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd |
| title_short | Genome-wide identification of bZIP gene family and expression analysis of BhbZIP58 under heat stress in wax gourd |
| title_sort | genome wide identification of bzip gene family and expression analysis of bhbzip58 under heat stress in wax gourd |
| topic | Wax gourd Heat stress Genome-wide identification Evolutionary pattern BhbZIP58 |
| url | https://doi.org/10.1186/s12870-023-04580-6 |
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